Controlled Release on Cardiovascular Stents Using Plasma-Enhanced Adhesion of Biodegradable Nanoparticles

Abstract

One of the most common solutions to treat ischemic heart disease nowadays is the implantation of drug eluting stents. Currently, new strategies are being developed to improve healing process, which includes pharmacological treatments or gene therapy. In this paper, we presented a proposal based on the use of poly(β-amino ester) (pBAE) nanoparticles.To enhance the release method, we used an approach based on attaching these nanoparticles on a polymeric coating. The process includes coating metallic cardiovascular stents with a thin and highly functionalized layer of pentafluorophenyl methacrylate (PFM) to which loaded nanoparticles are chemically bonded. Through this design, when a stent is expanded during its implantation, nanoparticles will stay attached to the polymer matrix. Nanocarriers will penetrate target adjacent cells, guaranteeing effective drug delivery. Results obtained show that this work opens a pathway for pharmacological and/or gene delivery systems based on the adhesion of pBAE nanoparticles prior to stent implantation. KEYWORDS: control release; cardiovascular stent; pentafluorophenyl methacrylate (PFM); gene delivery; poly(β-amino ester) (pBAE) ABBREVIATIONS CAD Coronary Artery Disease CDCP Centres for Disease Control and Prevention DES Drug eluting stents BMS Bare metal stent PBAE Poly(β-amino ester) MiRNA Micro RNA SiRNA Small interfering RNA RNAi Interfering RNA PFM Pentafluorophenyl methacrylate GFP Green fluorescent plasmid PGFP Green fluorescent protein vector SEM Scanning electron microscopy AFM Atomic force microscopy Open Access