2007
DOI: 10.1016/j.jconrel.2007.05.018
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Controlled release of PEI/DNA complexes from mannose-bearing chitosan microspheres as a potent delivery system to enhance immune response to HBV DNA vaccine

Abstract: A novel approach involving the preparation of mannose-bearing chitosan microspheres with entrapping complexes of HBV DNA and PEI was developed to improve the delivery of DNA into antigen-presenting cells (APCs) after intramuscular (i.m.) injection. Compared with the traditional chitosan microspheres, the microspheres could quickly release intact and penetrative PEI/DNA complexes. What's more, chitosan was modified with mannose to target the primary APCs such as dendritic cells (DCs) owing to the high density o… Show more

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Cited by 88 publications
(53 citation statements)
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“…Many studies show that properties such as chemical structure, size, and morphology of nano and microgels can be easily controlled by crosslinking processes such as physical crosslinking, [20][21][22] irradiation-induced crosslinking, [23][24][25] chemical crosslinking based on polyaddition reactions, [26][27][28][29] and polymerization methods (precipitation polymerization [30][31][32][33][34] or miniemulsion polymerization [35][36][37][38][39] ). Based on the typical microgel properties, one can predict the following advantages when using them as microreactors or -carriers in comparison with other template systems: (1) easy preparation; (2) variable size and flexible functionalization by reactive groups; (3) highly porous structure with adjustable degree of crosslinking; (4) enhanced colloidal stability; and (5) stimuli-responsive change of the microgel dimension (T-, pH-sensitivity).…”
Section: Introductionmentioning
confidence: 99%
“…Many studies show that properties such as chemical structure, size, and morphology of nano and microgels can be easily controlled by crosslinking processes such as physical crosslinking, [20][21][22] irradiation-induced crosslinking, [23][24][25] chemical crosslinking based on polyaddition reactions, [26][27][28][29] and polymerization methods (precipitation polymerization [30][31][32][33][34] or miniemulsion polymerization [35][36][37][38][39] ). Based on the typical microgel properties, one can predict the following advantages when using them as microreactors or -carriers in comparison with other template systems: (1) easy preparation; (2) variable size and flexible functionalization by reactive groups; (3) highly porous structure with adjustable degree of crosslinking; (4) enhanced colloidal stability; and (5) stimuli-responsive change of the microgel dimension (T-, pH-sensitivity).…”
Section: Introductionmentioning
confidence: 99%
“…[4] One of the most efficient non-viral vectors in vitro and in vivo is the cationic polymer, polyethylenimine (PEI). [11][12][13][14][15][16][17][18][19][20][21][22][23][24][25] PEI is a water-soluble polymer in which the repeat unit of PEI is two carbon atoms followed by a nitrogen atom. Under physiological conditions, approximately 20% of the nitrogens are protonated.…”
Section: Introductionmentioning
confidence: 99%
“…Mannosylated chitosan/DNA complexes were more effi cient in transfecting DCs, as compared to water-soluble chitosan/DNA, and induced better INF-Ī³ production from DCs [ 95 ]. Mannosylated-chitosan-entrapping PEI/HBV-DNA complexes induced signifi cantly enhanced serum antibody production and CTL levels after intramuscular immunization [ 96 ]. Biotinylated chitosan nanoparticles were modifi ed with bifunctional fusion protein (bfFp) vectors for achieving DC-selective targeting.…”
Section: Chitosanmentioning
confidence: 99%