Controlled release of PEI/DNA complexes from mannose-bearing chitosan microspheres as a potent delivery system to enhance immune response to HBV DNA vaccine

Zhou, Xianfeng; Liu, Bin; Yu, Xianghui; Zha, Xiao; Zhang, Xizhen; Chen, Yu; Wang, Xueyun; Jin, Yinghua; Wu, Yongge; Chen, Yue; Shan, Yaming; Chen, Yan; Liu, Junqiu; Kong, Wei; Shen, Jiacong

doi:10.1016/j.jconrel.2007.05.018

Cited by 86 publications

(51 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Many studies show that properties such as chemical structure, size, and morphology of nano and microgels can be easily controlled by crosslinking processes such as physical crosslinking, [20][21][22] irradiation-induced crosslinking, [23][24][25] chemical crosslinking based on polyaddition reactions, [26][27][28][29] and polymerization methods (precipitation polymerization [30][31][32][33][34] or miniemulsion polymerization [35][36][37][38][39] ). Based on the typical microgel properties, one can predict the following advantages when using them as microreactors or -carriers in comparison with other template systems: (1) easy preparation; (2) variable size and flexible functionalization by reactive groups; (3) highly porous structure with adjustable degree of crosslinking; (4) enhanced colloidal stability; and (5) stimuli-responsive change of the microgel dimension (T-, pH-sensitivity).…”

Section: Introductionmentioning

confidence: 99%

Synthesis of hybrid microgels by coupling of laser ablation and polymerization in aqueous medium

Nachev

Zand

Coger

et al. 2012

Journal of Laser Applications

View full text Add to dashboard Cite

Loading microgels with bioactive nanoparticles (NPs) often requires multiple synthesis and purification steps, and organic solvents or precursors that are difficult to remove from the gel. Hence, a fast and aqueous synthesis procedure would facilitate the synthesis of inorganic–organic hybrid microgels. Two microgel compounds were hybridized with laser-generated zinc oxide (ZnO) NPs prepared in a single-step procedure. ZnO NPs were formed by laser ablation in liquid, while the polymer microgels were synthesized in-situ inside the ablation chamber. Further, the authors report the preparation of two different microgel systems. The first one was produced without the use of chemical initiator forming hydrogels with ZnO NPs and diffuse morpholgy. Typical microgel colloids were also synthesized via a conventional chemical method in a preheated reaction chamber. The existence of microgel colloids partially loaded with ZnO NPs was confirmed in a transmission electron microscopy investigation. Fourier transform infrared spectroscopic measurements and dynamic light scattering verify the formation of polymer colloids. These initial results indicate the application potential of laser ablation in microgel precursor solution for the fabrication of polymeric carriers for inorganic nanoparticles. Preliminary biological tests using zinc chloride demonstrated negative dose effects on primary cell culture with zinc concentrations above 200 μM but no noticeable influence at 100 μM.

show abstract

Section: Introductionmentioning

confidence: 99%

Synthesis of hybrid microgels by coupling of laser ablation and polymerization in aqueous medium

Nachev

Zand

Coger

et al. 2012

Journal of Laser Applications

View full text Add to dashboard Cite

show abstract

“…[4] One of the most efficient non-viral vectors in vitro and in vivo is the cationic polymer, polyethylenimine (PEI). [11][12][13][14][15][16][17][18][19][20][21][22][23][24][25] PEI is a water-soluble polymer in which the repeat unit of PEI is two carbon atoms followed by a nitrogen atom. Under physiological conditions, approximately 20% of the nitrogens are protonated.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the transgene expression generated by branched and linear polyethylenimine-plasmid DNA nanoparticles in vitro and after intraperitoneal injection in vivo

Intra¹,

Salem²

2008

Journal of Controlled Release

124

119

View full text Add to dashboard Cite

Polyethylenimine (PEI) is a cationic polymer that has shown significant potential for delivering genes in vitro and in vivo. Mixing cationic PEI with negatively charged plasmid DNA (pDNA) results in the spontaneous electrostatic formation of stable nanoparticle complexes. The structure of PEI can be branched or linear. In this study, we show that branched PEI has a stronger electrostatic interaction with pDNA than linear PEI, which accounts for greater compaction, higher zeta potentials and smaller nanoparticle sizes at equivalent pDNA concentrations. For both linear and branched PEI, increasing the concentration of pDNA mixed in the same volume and at the same nitrogen to phosphate (N:P) ratio results in larger average particle sizes. Increasing the N:P ratio increases luciferase activity generated by branched PEI-pDNA nanoparticles and linear PEI-pDNA nanoparticles in HEK293, COS7 and HeLa cell lines. Increasing the N:P ratio at which branched PEI-pDNA nanoparticles are prepared also increases luciferase expression in HepG2 cells but does not increase luciferase expression generated by linear PEI-pDNA nanoparticles. In all of the cell lines, branched PEI-pDNA nanoparticles prepared at N:P ratios of 10 and above generated significantly higher luciferase activity than linear PEI-pDNA nanoparticles. Luciferase activity was highest in the HEK293 cells and luciferase expression in each of the cell lines followed the order of HEK293

show abstract

“…Mannosylated chitosan/DNA complexes were more effi cient in transfecting DCs, as compared to water-soluble chitosan/DNA, and induced better INF-γ production from DCs [ 95 ]. Mannosylated-chitosan-entrapping PEI/HBV-DNA complexes induced signifi cantly enhanced serum antibody production and CTL levels after intramuscular immunization [ 96 ]. Biotinylated chitosan nanoparticles were modifi ed with bifunctional fusion protein (bfFp) vectors for achieving DC-selective targeting.…”

Section: Chitosanmentioning

confidence: 99%

Polymer-Based DNA Delivery Systems for Cancer Immunotherapy

David

Golani-Armon

2016

Advances in Delivery Science and Technology

View full text Add to dashboard Cite

The use of gene delivery systems for the expression of antigenic proteins is an established means for activating a patient's own immune system against the cancer they carry. Since tumor cells are poor antigen-presenting cells, crosspresentation of tumor antigens by dendritic cells (DCs) is essential for the generation of tumor-specifi c cytotoxic T-lymphocyte responses. A number of polymer-based nanomedicines have been developed to deliver genes into DCs, primarily by incorporating tumor-specifi c, antigen-encoding plasmid DNA with polycationic molecules to facilitate DNA loading and intracellular traffi cking. Direct in vivo targeting of plasmid DNA to DC surface receptors can induce high transfection effi ciency and long-term gene expression, essential for antigen loading onto major histocompatibility complex molecules and stimulation of T-cell responses. This chapter summarizes the physicochemical properties and biological information on polymer-based non-viral vectors used for targeting DCs, and discusses the main challenges for successful in vivo gene transfer into DCs.

show abstract

Controlled release of PEI/DNA complexes from mannose-bearing chitosan microspheres as a potent delivery system to enhance immune response to HBV DNA vaccine

Cited by 86 publications

References 33 publications

Synthesis of hybrid microgels by coupling of laser ablation and polymerization in aqueous medium

Synthesis of hybrid microgels by coupling of laser ablation and polymerization in aqueous medium

Characterization of the transgene expression generated by branched and linear polyethylenimine-plasmid DNA nanoparticles in vitro and after intraperitoneal injection in vivo

Polymer-Based DNA Delivery Systems for Cancer Immunotherapy

Contact Info

Product

Resources

About