Controlled Release of Lysozyme Using Polyvinyl Alcohol-Based Polymeric Nanofibers Generated by Electrospinning

Abstract

Polymeric nanofibers generated via electrospinning offer a promising platform for drug delivery systems. This study examines the application of electrospun polyvinyl alcohol (PVA) nanofibers for controlled lysozyme (LZM) delivery. By using various PVA grades, such as the degree of polymerization/hydrolysis, this study investigates their influence on nanofiber morphology and drug-release characteristics. LZM-loaded PVA monolithic nanofibers having 50% drug content exhibit efficient entrapment, wherein rapid dissolution is achieved within 30 min. The initial burst of LZM from the nanofiber was reduced as the LZM content was lowered. The initial dissolution is greatly influenced by the choice of PVA grade used; fully hydrolyzed PVA nanofibers demonstrate controlled release due to the reduced water solubility of PVA. Furthermore, coaxial electrospinning, which creates core-shell nanofibers with polycaprolactone as a controlled release layer, enables sustained LZM release over an extended period. This study confirms a correlation between PVA characteristics and controlled drug release and provides valuable insights into tailoring nanofiber properties for pharmaceutical applications.

Development of Polyvinyl Alcohol (PVA) Nanofibers Containing Cationic Lipid/siRNA Complexes via Electrospinning: The Impact of PVA Characterization

“…The siRNA encapsulation efficiency in PVA nanofibers, measured using HPLC, was 94.5 ± 1.5%. This elevated drug encapsulation efficiency in electrospun fibers could be attributed to the drug and polymer solubilities in water, thus minimizing drug loss during encapsulation [31,32]. Incorporation of either siRNA or DOTAP/siRNA complexes (N/P ratio = 5) slightly reduced the fiber diameters to 382 ± 43 nm and 311 ± 88 nm, respectively (Figure 1b and c), presumably because of the enhanced conductivity of the PVA solution [33].…”