Controlled Release of IgG by Novel UV Induced Polysaccharide/Poly(amino acid) Hydrogels

Abstract

The development of new protein and peptide drugs needs new delivery systems able to entrap such drugs in safe conditions without affecting their structure and biological activity. In this context, the present work reports a new approach to load IgG, used as a model of therapeutic proteins such as anti-TNF-alpha monoclonal antibodies, into a polymeric system able to release the entrapped IgG in a controlled manner. In particular, new polysaccharide/poly(amino acid) UV induced hydrogels are proposed as colon delivery systems for human IgG. The poly(amino acid), alpha,beta-poly[N-(2-hydroxyethyl)-D,L-aspartamide], has been functionalized with methacrylic anhydride, while the polysaccharide, inulin, has been functionalized with methacrylic anhydride and succinic anhydride. The hydrogels were obtained by a short-time UV irradiation, in physiological-like conditions, without the use of radical initiators, at low temperature and in the presence or in the absence of PEGDM(550) used as a co-crosslinker in order to evaluate potential differences in terms of physicochemical properties and release profile. The obtained hydrogels were degradable by inulinase, showed a high cell compatibility and the released antibodies, analyzed by SEC and ELISA, retained their biological activity.

In‐Solution Structural Considerations by 1H NMR and Solid‐State Thermal Properties of Inulin‐d‐α‐Tocopherol Succinate (INVITE) Micelles as Drug Delivery Systems for Hydrophobic Drugs

“…Classic examples of polymers used in drug delivery are polysaccharides, polyaminoacids, polyethers, and polyesters . Among these, polysaccharides from renewable sources are widely studied for pharmaceutical applications and, in particular, to build up innovative drug delivery systems . One of the most important pharmaceutical applications of polymers is the formation of macro­molecular amphiphiles to form specific self‐assembling systems for the drug delivery of poorly water‐soluble drugs.…”

“…INVITE polymers are synthesized by conjugating the d ‐α‐tocopherol succinate (VITE) to the polyfructose Inulin (INU). INU is a water‐soluble polysaccharide that resulted extremely versatile for pharmaceutical applications while VITE is a hydrophobic vitamin with antioxidant activities leading to amphiphiles after conjugation with hydrophilic polymers …”

Nanostructured Polymeric Functional Micelles for Drug Delivery Applications

“…In the last years, INU found several applications as a valuable polymer for drug delivery purposes. It has been proposed for the formulation and production of different drug delivery systems such as hydrogels for colon targeted drug delivery, or for the therapy of iron deficiency anemia …”

New Perspectives in Cancer Therapy: The Biotin-Antitumor Molecule Conjugates

“…One of the most common consists in providing the drug with a suitable carrier. The polymeric drug carriers emerged as the most effective ones because of their versatility of use as well as their straightforward chemical modification [9][10][11][12][13][14][15][16][17]. These systems usually bring to a passive drug targeting due to their ability to accumulate in a specific organ as a result of their route of elimination or for particular organotropism.…”