Controlled Release of Gentamicin from Electrospun Poly(Vinyl Alcohol)/Gelatin Nanofibers: The Effect of Crosslinking Time Using Glutaraldehyde Vapor

Abstract

In this study, polyvinyl alcohol (PVA), gelatin (GEL) and gentamicin (GEN) were used and 13PVA/0.5GEL/GEN nanofibers were fabricated with the electrospinning method. These nanofibers were crosslinked chemically with glutaraldehyde (GA) at different time intervals (2, 3, 4, 5, 5.5 and 6 h) to observe the crosslinking effect on the properties of the nanofibers. Morphological analysis reported that as the crosslinking time increased, the nanofiber diameters also increased from 369.26 nm (non‐crosslinked) to 447.72 nm (6 h/crosslinking), respectively. The thermal characterization results demonstrated that crosslinking with different times noticeably shifted the thermal points.The tensile testing results proved that application of crosslinking enhanced the mechanical strength of the nanofibers from 3.31 MPa (non‐crosslinked) to 5.8 MPa (5.5 h/crosslinking), respectively. The GEN release profiles from the nanofibers showed similar behaviors under crosslinking and indicated that the crosslinking time did not have a significant effect on the amount of the GEN released.

Improvement in the Sustained-Release Performance of Electrospun Zein Nanofibers via Crosslinking Using Glutaraldehyde Vapors

“…The SEM images show that the fibers of GTA_0h were continuous and uniform. After crosslinking with GTA vapor, the fibers swelled but retained their fiber morphology, which is considered to be related to the absorption of water vapor during exposure to GTA vapors [35]. These results are consistent with those of previous studies, in which the fiber diameter of electrospun nanofibers increased after vapor-phase GTA crosslinking [35,52].…”

“…Several studies have investigated the effect of GTA vapor crosslinking on the release performance of electrospun nanofibers [34]. Baykara et al (2022) reported that the vapor-phase crosslinking of GTA on PVA/gelatin nanofibers did not significantly affect the release of gentamicin [35]. Hadyz et al (2021) reported that chitosan/PEO nanofibers crosslinked by vapors of GTA extended the release time of nizatidine [33].…”

Machine intelligence accelerated design of conductive MXene aerogels with programmable properties

“…During the robot-assisted mixture preparation, an optional step is to incorporate GA as a crosslinking agent into the MXene/CNF/gelatin mixtures. GA is widely acknowledged as a chemical crosslinker for CNFs 68 , gelatin 69 , and MXene nanosheets 30 , 36 . To investigate possible covalent bonds formed between CNFs and/or MXene nanosheets, two kinds of MXene/CNF aerogels (at the 80/20 ratio and at 10 mg mL –1 ) were produced: one incorporated with GA (denoted as “+”) and the other without GA (as “–”).…”

Functional Gel-Based Electrochemical Energy Storage

“…A classical approach for this method uses a cross-linker, a molecule that links the polymer chains to form the 3D matrix. Some examples of cross-linkers are glutaraldehyde, 140 ethylene glycol, 141 and citric acid. 142 In this method, the mechanical resistance of hydrogels increases substantially when compared to physical methods due to the more stable interaction between the polymeric chains.…”