Controlled release of antibody proteins from liquid crystalline hydrogels composed of genetically engineered filamentous viruses

Sawada, Tatsuo; Yanagimachi, Miyuki; Serizawa, Takeshi

doi:10.1039/c6qm00140h

Cited by 15 publications

(14 citation statements)

References 37 publications

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“…7 Importantly, genetic engineering for M13 phage to display foreign peptides or proteins for the evolutional screening process has been well established; 8 therefore, the resultant M13 phages with desired functions are utilized as versatile building blocks for virus-based functional so materials for application in electronic devices, sensors, and biomedical scaffolds as well as conventional antibacterial reagents and drug delivery vehicles. [9][10][11][12][13][14][15][16][17][18][19][20][21] Viruses have protein shells that assemble several copies of the same protein into assemblies (called capsids) with precisely controlled three-dimensional structures. Their genetic code in the form of DNA or RNA is located in the interior of the coat protein-assemblies, and electrostatic interactions between the genetic material and surrounding proteins are essential to form these ordered structures.…”

Section: Introductionmentioning

confidence: 99%

Controlled assembly of filamentous viruses into hierarchical nano- to microstructures at liquid/liquid interfaces

Tanaka

Sawada

et al. 2020

RSC Adv.

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Section: Introductionmentioning

confidence: 99%

Controlled assembly of filamentous viruses into hierarchical nano- to microstructures at liquid/liquid interfaces

Tanaka

Sawada

et al. 2020

RSC Adv.

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“…M13 bacteriophage (phage), one of the filamentous viruses, is a regular assembly of plural proteins and genetic DNA. Recently, it has been reported that the phages can be utilized as a material component in various fields due to their capabilities of molecular recognition 13 – 19 , catalysis 20 , 21 , and nanoparticle nucleation 22 , 23 . These functions are easily integrated into the phages by genetic 24 or chemical 25 modification of the coat proteins on surfaces.…”

Section: Introductionmentioning

confidence: 99%

Filamentous Virus-based Assembly: Their Oriented Structures and Thermal Diffusivity

Sawada

Murata

Marubayashi

et al. 2018

Sci Rep

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Organic polymers are generally regarded as thermal insulators because amorphous arrangement of molecular chains reduces the mean free path of heat-conducting phonons. However, recent studies indicated that single chains of polymers with highly oriented structures could have high thermal conductivity than bulk polymers because stretched polymer chains effectively conduct phonons through polymeric covalent bonds. Here, we demonstrated the possibility of non-covalent virus assembly prepared by simple flow-induced methods toward high thermal conductive polymeric materials. Films with high thermal diffusivity composed of non-covalent bond-based assemblies of liquid crystalline filamentous viruses were prepared using a simple flow-induced orientation method. Structural and thermal characterization demonstrated that highly oriented structures of the viruses in the film were attributed to the high thermal diffusivity. Our results will open attractive opportunities for biomolecular-based thermally conductive soft materials even though the assemblies are based on non-covalent bonds.

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“…These hydrogels were also cast into gel strings by injecting into a 5% glutaraldehyde solution, thus combining physical and chemical cross-linking (Figure F) . Hybrid hydrogels were also made by physically cross-linked gelatin and liquid-crystalline M13 phages or physically cross-linking phages displaying hydroxyapatite (HA)-binding peptides with gelatin (used for controlled-release of HA antibodies) …”

Section: History and Current Status Of Filamentous Phage Hydrogelsmentioning

confidence: 99%

Filamentous Phages as Building Blocks for Bioactive Hydrogels

Jackson

Peivandi

Fogal

et al. 2021

ACS Appl. Bio Mater.

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Filamentous bacteriophages (bacterial viruses) are semiflexible proteinous nanofilaments with high aspect ratios for which the surface chemistry can be controlled with atomic precision via genetic engineering. That, in addition to their ability to self-propagate and replicate a nearly monodisperse batch of biologically and chemically identical nanofilaments, makes these bionanofilaments superior to most synthetic nanoparticles and thus a powerful tool in the bioengineers’ toolbox. Furthermore, filamentous phages form liquid crystalline structures at high concentrations; these ordered assemblies create hierarchically ordered macro-, micro-, and nanostructures that, once cross-linked, can form hierarchically ordered hydrogels, hydrated soft material with a variety of physical and chemical properties suitable for biomedical applications (e.g., wound dressings and tissue engineering scaffolds) as well as biosensing, diagnostic assays. We provide a critical review of these hydrogels of filamentous phage, and their physical, mechanical, chemical, and biological properties and current applications, as well as an overview of limitations and challenges and outlook for future applications. In addition, we present a list of design parameters for filamentous phage hydrogels to serve as a guide for the (bio)engineer and (bio)chemist interested in utilizing these powerful bionanofilaments for designing smart, bioactive materials and devices.

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Controlled release of antibody proteins from liquid crystalline hydrogels composed of genetically engineered filamentous viruses

Abstract: Controlled release of antibody proteins from hydrogels composed of liquid crystalline filamentous viruses and physically cross-linked gelatin was demonstrated.

Cited by 15 publications

References 37 publications

Controlled assembly of filamentous viruses into hierarchical nano- to microstructures at liquid/liquid interfaces

Controlled assembly of filamentous viruses into hierarchical nano- to microstructures at liquid/liquid interfaces

Filamentous Virus-based Assembly: Their Oriented Structures and Thermal Diffusivity

Filamentous Phages as Building Blocks for Bioactive Hydrogels

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