Controlled-release formulation of antihistamine based on cetirizine zinc-layered hydroxide nanocomposites and its effect on histamine release from basophilic leukemia (RBL-2H3) cells

Abstract

Abstract:A controlled-release formulation of an antihistamine, cetirizine, was synthesized using zinc-layered hydroxide as the host and cetirizine as the guest. The resulting well-ordered nanolayered structure, a cetirizine nanocomposite "CETN," had a basal spacing of 33.9 Å, averaged from six harmonics observed from X-ray diffraction. The guest, cetirizine, was arranged in a horizontal bilayer between the zinc-layered hydroxide (ZLH) inorganic interlayers. Fourier transform infrared spectroscopy studies indicated that the intercalation takes place without major change in the structure of the guest and that the thermal stability of the guest in the nanocomposites is markedly enhanced. The loading of the guest in the nanocomposites was estimated to be about 49.4% (w/w). The release study showed that about 96% of the guest could be released in 80 hours by phosphate buffer solution at pH 7.4 compared with about 97% in 73 hours at pH 4.8. It was found that release was governed by pseudo-second order kinetics. Release of histamine from rat basophilic leukemia cells was found to be more sensitive to the intercalated cetirizine in the CETN compared with its free counterpart, with inhibition of 56% and 29%, respectively, at 62.5 ng/mL. The cytotoxicity assay toward Chang liver cells line show the IC 50 for CETN and ZLH are 617 and 670 μg/mL, respectively.

Anticancer nanodelivery system with controlled release property based on protocatechuate–zinc layered hydroxide nanohybrid

“…13,26 About 0.2 g of ZnO was reacted with 100 mL of 0.1 mol/L protocatechuic acid solution. The solution was titrated with l mol/L NaOH to the final pH of 7.9 under vigorous stirring.…”

“…This result is similar to the kinetic study for the release of chlorogenic acid from ZLH layers, 13 camptothecin from Mg/Al-LDH, 46 and cetirizine from ZLH. 48 The correlation coefficient and release rate constant at pH 4.8 ( Figure 10E) are 0.9966 and 5.86×10…”

Sustained release formulation of an anti-tuberculosis drug based on para-amino salicylic acid-zinc layered hydroxide nanocomposite

“…The intercalation of hippuric acid into ZLH has resulted in the less toxic of the compound compared to the pure hippuric acid itself [15]. It was also reported that the controlled release formulation of an antihistamine was obtained by forming the cetirizine-ZLH nanocomposite [16]. …”

Hippuric acid nanocomposite enhances doxorubicin and oxaliplatin-induced cytotoxicity in MDA-MB231, MCF-7 and Caco2 cell lines

“…The hippuric acid nanocomposite (HAN) was synthesized by the direct method using ZnO as the starting material as reported previously,9,13,14 with minor modifications. The hippuric acid solution (0.01 Mole) was prepared using 1.8 g of hippuric acid in 20 mL of DMSO, and was adjusted to 50 mL by the addition of deionized water.…”