Controlled release and antibacterial activity chlorhexidine acetate (CA) intercalated in montmorillonite

Meng, Na; Zhou, Ninglin; Zhang, Shuangquan; Shen, Jian

doi:10.1016/j.ijpharm.2009.08.004

Cited by 96 publications

(48 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Basal planes of MMT are also known to bind to positively charged molecules, including cationic antibiotics, via an exchange with native cations. 35,36 To confirm the binding of gentamicin with MMT nanoplatelets, we directly assembled gentamicin and MMT from pH 7.5 solutions and used these films as a control. Supplementary Figure S4 shows that unlike (MMT/PAA) n films, the MMT/gentamicin films were very rough (Supplementary Figure S4), suggesting uneven deposition of components.…”

Section: Responsive Nanocomposite Assembliesmentioning

confidence: 99%

Small-molecule-hosting nanocomposite films with multiple bacteria-triggered responses

et al. 2014

View full text Add to dashboard Cite

We report pH/bacteria-responsive nanocomposite coatings with multiple mechanisms of antibacterial protection that include the permanent retention of antimicrobials, bacteria-triggered release of antibiotics and bacteria-induced film swelling. A novel small-molecule-hosting film was constructed using layer-by-layer deposition of montmorillonite (MMT) clay nanoplatelets and polyacrylic acid (PAA) components, both of which carry a negative charge at neutral pH. The films were highly swollen in water, and they exhibited major changes in swelling as a function of pH. Under physiologic conditions (pH 7.5, 0.2 M NaCl), hydrogel-like MMT/PAA films took up and sequestered B45% of the dry film matrix mass of the antibiotic gentamicin, causing dramatic film deswelling. Gentamicin remained sequestrated within the films for months under physiologic conditions and therefore did not contribute to the development of antibiotic resistance. When challenged with bacteria (Staphylococcus aureus, Staphylococcus epidermidis or Escherichia coli), the coatings released PAA-bound gentamicin because of bacteria-induced acidification of the immediate environment, whereas gentamicin adsorbed to MMT nanoplatelets remained bound within the coating, affording sustained antibacterial protection. Moreover, an increase in film swelling after gentamicin release further hindered bacterial adhesion. These multiple bacteria-triggered responses, together with nontoxicity to tissue cells, make these coatings promising candidates for protecting biomaterial implants and devices against bacterial colonization. INTRODUCTIONPolymer nanocomposites uniquely combine the properties of inorganic and organic components that is central to the development of novel advanced materials. The naturally occurring smectite clays, which are chemically and thermally stable and biocompatible, are attractive nanocomposites. Exfoliated clay nanoplatelets strongly impact the mechanical, transport, optical, fire retardant and gas barrier properties of nanocomposite materials. 1,2 For many biomedical applications, however, hydrophilic rather than hydrophobic nanocomposites are of great interest, and responsive properties are essential. 3 Reports on responsive hydrophilic nanocomposites are rare, and those few known to us advantageously use the complementary properties of temperature/pH-responsive polymers and clay nanosheets to yield environmentally controlled free-standing materials. 4 A promising way to leverage the favorable properties of hydrophilic-responsive nanocomposites on surfaces is to use the layer-by-layer (LbL) technique to construct 'smart' surface coatings. 5 To construct clay-containing LbL films, electrostatic interactions of positively charged polymers with negatively charged basal planes of silicate nanosheets, 6,7 neutral polymer/nanoplatelet hydrogen bonding 8 or a combination of the two have been explored. 9

show abstract

Section: Responsive Nanocomposite Assembliesmentioning

confidence: 99%

Small-molecule-hosting nanocomposite films with multiple bacteria-triggered responses

et al. 2014

View full text Add to dashboard Cite

show abstract

“…The MMTNa suspension did show any inhibition activity, which corroborates results shown by other authors. 11,24,26,27 These authors not only tested sodium but also calcium montmorillonite. Thus, any synergic effect can be attributed to the absorptive properties of clay.…”

Section: Evaluation Of Antimicrobial Activitymentioning

confidence: 99%

“…Therefore, inclusion processes can occur with several different cations or small molecules, leading to substance encapsulation in the interlamellar space, increasing system tortuosity and water uptake. 12 The use of MMTNa for acetate chlorhexidine inclusion has been previously reported, 11 as has the use of βCD for CHX and NYS encapsulation. 4,10 However, the use of water-soluble methyl-β-cyclodextrin (MβCD) and hydroxypropyl-β-cyclodextrin (HPβCD) for CHX, and MMTNa for both drugs, are other possibilities to be studied in order to obtain stable formulations with tolerable taste.…”

mentioning

confidence: 97%

“…4,10 However, better results can be obtained using nanocarriers with sustained release properties, such as sodium montmorillonite (MMTNa), the main constituent of bentonite (60%). This nanocarrier may present some synergistic effect due to the capability of these materials to adsorb and fix bacteria and fungi, immobilizing their toxins, 11 and also presents parallel lamellae with internal anionic surfaces and interlamellar cations for equilibrium and charge stabilization. Therefore, inclusion processes can occur with several different cations or small molecules, leading to substance encapsulation in the interlamellar space, increasing system tortuosity and water uptake.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Preparation and evaluation of antimicrobial activity of nanosystems for the control of oral pathogens Streptococcus mutans and Candida albicans

Cg¹,

Villardi²,

Cr³

et al. 2011

IJN

View full text Add to dashboard Cite

Background Diseases that affect the buccal cavity are a public health concern nowadays. Chlorhexidine and nystatin are the most commonly used drugs for the control of buccal affections. In the search for more effective antimicrobials, nanotechnology can be successfully used to improve the physical chemical properties of drugs whilst avoiding the undesirable side effects associated with its use. Herein described are studies using nystatin and chlorhexidine with sodium montmorillonite (MMTNa), and chlorhexidine with β-cyclodextrin and two derivatives methyl-β-cyclodextrin and hydroxypropyl-β-cyclodextrin in the development of antimicrobial nanosystems. Methods The nanosystems were prepared by kneading and solubilization followed by freeze-drying technique. The nanosystems were characterized by X-ray powder diffraction (XRPD), differential scanning calorimetry (DSC), and Fourier transform infrared spectroscopy (FTIR). Nanosystem antimicrobial activity against Streptococcus mutans and Candida albicans strains was evaluated with inhibition halo analysis. Results The nanocarriers MMTNa and cyclodextrins showed good yields. XRPD, FTIR, and DSC analysis confirmed the proposed nanosystems formation and the suitability of the production methods. The nanosystems that showed best antimicrobial effect were chlorhexidine gluconate (CHX) and cyclodextrin inclusion complexes and CHX:MMTNa 60% cation exchange capacity – 24 hours. Conclusion The nanosystem formulations present higher stability for all chlorhexidine inclusion complexes compared with pure chlorhexidine. The nystatin nanosystems have the potential to mask the bitter taste, justifying subsequent in-vivo studies. For these reasons, further studies are being carried out to evaluate their application in professional formulations.

show abstract

“…Various studies have shown that montmorillonite is one of the inorganic materials that provides promising opportunities in biomedical applications [5][6][7][8]. Since the knowledge of their physical and chemical properties, the applications of montmorillonites were primarily focused on drug delivery systems [9]. The use of montmorillonite as a carrier of drugs has been reported by some researchers such as chlorhexidine acetate [10], platinum anticancer complex in vitro Controlled Drug Release of Antidiabetic Metformin HCl from Citric Acid Activated Natural Montmorillonite [11], gallic acid [12] and gentamycin [13].…”

Section: Introductionmentioning

confidence: 99%

in vitro Controlled Drug Release of Antidiabetic Metformin HCl from Citric Acid Activated Natural Montmorillonite

Putra¹,

Sitanggang²,

Suarya³

et al. 2017

Asian J. Chem.

View full text Add to dashboard Cite

In this work, metformin HCl was encapsulated on citric acid activated montmorillonite as drug delivery system. The as-synthetized drug delivery systems were characterized by using FTIR and X-ray diffraction. FTIR spectra showed the presence of bending vibration of N-H from primary amines group and streching vibration of C=N at 1635.64 and 1506.41 cm -1 , respectively. X-ray diffraction pattern also showed a widening of the diffraction peaks of montmorillonite after encapsulated by metformin HCl. In vitro drug release results showed that the release of metformin HCl from the drug delivery system in simulated intestinal fluid (pH 7.4) higher than that in simulatied gastric fluid (pH 1.2) with a value of drug release of 61.44 % and 39.41 %. Metformin HCl release kinetics at each pH follow the zero-order model (pH 1.2 with R = 0.9924; pH 7.4 with R = 0.9972) suggested diffusion-controlled release mechanism.

show abstract

Controlled release and antibacterial activity chlorhexidine acetate (CA) intercalated in montmorillonite

Cited by 96 publications

References 25 publications

Small-molecule-hosting nanocomposite films with multiple bacteria-triggered responses

Small-molecule-hosting nanocomposite films with multiple bacteria-triggered responses

Preparation and evaluation of antimicrobial activity of nanosystems for the control of oral pathogens Streptococcus mutans and Candida albicans

in vitro Controlled Drug Release of Antidiabetic Metformin HCl from Citric Acid Activated Natural Montmorillonite

Contact Info

Product

Resources

About