Controlled‐rate versus uncontrolled‐rate freezing as predictors for platelet cryopreservation efficacy

Balint, Bela; Paunovic, Dragica; Vucetic, Dusan; Vojvodić, Danilo; Petakov, Marijana; Trkuljic, Miroljub; N, Stojanović

doi:10.1111/j.1537-2995.2006.00706.x

Cited by 19 publications

(23 citation statements)

References 23 publications

(25 reference statements)

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“…P‐selectin is stored in granules of platelets and translocated to the platelet surface upon cellular activation. As shown in this and previous studies, P‐selectin expression was markedly up regulated following cryopreservation . Similar to the response to PS blockage, when P‐selectin on CPPs was blocked by anti–P‐selectin, the phagocytic activity and release of inflammatory cytokines in THP‐1 cells coincubated with CPPs were also partially inhibited, although the inhibitory effects were slighter compared with PS (Fig.…”

Section: Discussionsupporting

confidence: 85%

Cryopreserved platelets augment the inflammatory response: role of phosphatidylserine‐ and P‐selectin–mediated platelet phagocytosis in macrophages

Zhao

Gan

et al. 2019

Transfusion

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BACKGROUND Cryopreservation in dimethyl sulfoxide and storage at −80 °C extends the shelf life of platelets to at least 2 years, allowing greater availability in rural and military areas. While cryopreserved platelets (CPPs) have been extensively characterized for coagulation and thrombin generation, reports on the mechanism of adverse reactions to CPPs transfusion are scarce. Here, we tested the hypothesis that CPPs facilitate phagocytosis by Kupffer cells and subsequently promote the inflammatory response in Kupffer cells. STUDY DESIGN AND METHODS P‐selectin expression, glycoprotein Ibα clustering and phosphatidylserine (PS) surface exposure on platelets stored at 22 °C, 4 °C and − 80 °C for 3 days were examined by flow cytometry. The phagocytosis of mepacrine‐labeled platelets coincubated with THP‐1 cells was examined by flow cytometry and confocal microscopy, and the release of cytokines from THP‐1 cells was measured by enzyme‐linked immunosorbent assay. RESULTS CPPs showed a marked enhancement of exposed PS but dramatically reduced glycoprotein Ibα expression and clustering compared with platelets stored at 4 °C. Activation of THP‐1 cells was stronger by CPPs than by platelets stored at 22 °C and 4 °C. CPP interference tests using annexin V and anti–P‐selectin showed that CPPs induced increases in PS‐ and P‐selectin–mediated phagocytosis, as well as secretion of the proinflammatory cytokine tumor necrosis factor‐α, and interleukins IL‐1β and IL‐6, but a decrease in transforming growth factor‐β production in THP‐1 cells. Surface‐exposed PS was more effective than P‐selectin for the activation of THP‐1 cells. CONCLUSION CPPs triggered PS and P‐selectin–mediated phagocytosis by macrophages and stimulated the inflammatory response of macrophages.

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Section: Discussionsupporting

confidence: 85%

Cryopreserved platelets augment the inflammatory response: role of phosphatidylserine‐ and P‐selectin–mediated platelet phagocytosis in macrophages

Zhao

Gan

et al. 2019

Transfusion

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“…14 Studies of 6% DMSO cryopreserved human platelets reported in vitro recoveries ranging from 50 to 80%. [15][16][17] Similar in vitro recoveries of 70-80% have been reported in studies of canine platelets cryopreserved in 6% DMSO. 4,9 In this study, 6% DMSO platelets had a mean in vitro recovery of 72.8%, significantly lower than that of Thrombosol platelets (95.2%, P 5 .02).…”

Section: Discussionsupporting

confidence: 73%

“…7,20,21 In studies of cryopreserved human platelets, baseline (or unstimulated) P-selectin expression was increased (35%) compared with fresh platelets (9%), suggesting premature platelet activation during the freezethaw-wash process. 17 Cryopreserved human platelets showed reduced P-selectin expression after ADP stimulation, reflecting their decreased ability for normal activation.…”

Section: Discussionmentioning

confidence: 99%

Cryopreservation of Canine Platelets

Appleman

Sachais

Patel

et al. 2009

Veterinary Internal Medicne

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Background: Platelet cryopreservation allows long-term storage and immediate availability of transfusion products. Hypothesis: The addition of a preparation inhibiting platelet activation (Thrombosol, in 2% dimethyl sulfoxide [DMSO]) will enhance in vitro function and prolong in vivo survival of cryopreserved platelets compared with those preserved in 6% DMSO.Animals: Thirty-three research dogs. Methods: Prospective study. Eleven fresh canine apheresis platelet concentrates (PCs) were each split into 3 units: fresh and cryopreserved in 6% DMSO or Thrombosol. Platelet analysis, performed 1-10 weeks postfreezing, included in vitro functional testing and in vivo survival assessed by administration of biotinylated platelets.Results: Platelet aggregation was diminished in cryopreserved PC. Cryopreserved platelets could be activated, as based on mean thrombin-stimulated P-selectin expression (6% DMSO, 23.0%; Thrombosol, 18.4%), although to a lesser extent than fresh PC (49.1%) (P o .0001). The mean maximum in vivo platelet recovery for fresh PC was 80.3%, significantly greater than recovery for 6% DMSO (49.2%) and Thrombosol PC (43.7%) (P .001). The half-life (days) of fresh PC (3.8 AE 0.4) was significantly (P o .002) greater than that of 6% DMSO (1.9 AE 1.0) and Thrombosol (2.4 AE 1.1) PC, with no difference (P 5 .3) between cryopreserved PC.Conclusions and Clinical Importance: Cryopreservation of canine platelets using Thrombosol did not provide any advantage over preservation using 6% DMSO. Cryopreserved platelets can be activated in vitro and provide therapeutic benefit when fresh platelets are unavailable. Further studies are needed to assess their in vivo hemostatic function.

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“…Many laboratories have reported good results using a controlled‐rate freezing approach (Borderie et al ., ; Balint et al ., ); however, a ‘quick freeze’ (often, but not always correctly, equated with ‘vitrification’) has been demonstrated to be at least as effective (Cetinkaya and Arat, ; Tan et al ., ) or superior in terms of cell quality (Kuleshova et al ., ; Berz et al ., ). Cells frozen using P7 showed no vitrified glass state by visual inspection.…”

Section: Discussionmentioning

confidence: 98%

Comparison of different cooling rates for fibroblast and keratinocyte cryopreservation

Naaldijk

Friedrich-Stöckigt

Sethe

et al. 2013

J Tissue Eng Regen Med

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Easy, cost-effective and reliable cryopreservation protocols are crucial for the successful and effective application of tissue engineering. Several different protocols are in use, but no comprehensive comparisons across different machine-based and manual methods have been made. Here, we compare the effects of different cooling rates on the post-thaw survival and proliferative capacity of two basic cell lines for skin tissue engineering fibroblasts and keratinocytes, cultured and frozen in suspension or as a monolayer. We demonstrate that effectiveness of cryopreservation cannot be reliably determined immediately after thawing: the results at this stage were not indicative of cell growth in culture 3 days post-thaw. Cryopreservation of fibroblasts in an adherent state greatly diminishes their subsequent growth potential. This was not observed when freezing in suspension. In keratinocytes, however, adherent freezing is as effective as freezing in suspension, which could lead to significant cost and labour savings in a tissue-engineering environment. The 'optimal' cryopreservation protocol depends on cell type and intended use. Where time, ease and cost are dominant factors, the direct freezing into a nitrogen tank (straight freeze) approach remains a viable method. The most effective solution across the board, as measured by viability 3 days post-thaw, was the commonly used, freezing container method. Where machine-controlled cryopreservation is deemed important for tissue-engineering Good Manufacturing Practice, we present results using a portfolio of different cooling rates, identifying the 'optimal' protocol depending on cell type and culture method. Copyright © 2013 John Wiley & Sons, Ltd.

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Controlled‐rate versus uncontrolled‐rate freezing as predictors for platelet cryopreservation efficacy

Cited by 19 publications

References 23 publications

Cryopreserved platelets augment the inflammatory response: role of phosphatidylserine‐ and P‐selectin–mediated platelet phagocytosis in macrophages

Cryopreserved platelets augment the inflammatory response: role of phosphatidylserine‐ and P‐selectin–mediated platelet phagocytosis in macrophages

Cryopreservation of Canine Platelets

Comparison of different cooling rates for fibroblast and keratinocyte cryopreservation

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