Controlled protein delivery from photosensitive nanoparticles

Abstract

Light provides a powerful approach for delivery of cargos and study of important biological events. This article reports a series of photosensitive and biocompatible delivery of nanoparticles which released proteins upon light irradiation. The nanoparticles were synthesized by emulsion copolymerization of 2-(dimethylamino) ethyl methacrylate with functional monomers and a photoliable o-nitrobenzyl diacrylate crosslinker. Upon mild UV irradiation (λ = 365 nm, 10 mJ/cm(2)), the photosensitive crosslinker underwent light-induced degradation and the sizes of the nanoparticles increased dramatically. The nanoparticles were uptaken by the cells which is confirmed by flow cytometry analysis. Bovine serum albumin and green fluorescent protein were loaded as model proteins into the nanoparticles and accelerated photo-triggered release were achieved.

Intracellular delivery of more than one protein with spatio-temporal control

“…18,20,21 Released siRNAs may mediate gene silencing in model cells. 18 Although light-triggered release of proteins has been reported, 12,22 no formulation has achieved the photo-triggered release of more than one protein from the same nanocarrier using a single wavelength. In this work, we have developed a nanomaterial and oligonucleotide chemistry for the intracellular delivery of two proteins with spatio-temporal control using NIR light as a trigger.…”

Stimulus Responsive Hydrogels for Targeted Cancer Therapy

“…This unique responsiveness to light makes these hydrogels invaluable for a wide range of applications, including drug delivery, tissue engineering, biosensing, and actuators in soft robotics, offering unprecedented levels of precision and versatility in biomedical and technological fields [129]. Drug release mechanisms including photo-induced transformation [130], bond cleavage [131], photoisomerization [132], or photo crosslinking [133] can be activated by UV light. Consequently, certain linkers containing aromatic are susceptible to UV light-induced drug release via one or more photon excitation mechanisms operating at specific wavelengths.…”

Photodegradable self-assembling PAMAM dendrons for gene delivery involving dendriplex formation and phototriggered circular DNA release

“…Recently, the concept of phototriggers has provided a useful strategy in photocontrolled drug delivery systems (PDDSs) because it enables rapid and accurate control over specific sites and times with external light stimulation. [15][16][17][18][19][20] Biologically relevant materials containing a photolabile building block can undergo efficient photolysis through active phototriggers, thus causing structural degradation and the release of biological targets. Among the numerous photolabile groups that have been studied, o-nitrobenzyl (o-NB) alcohol derivatives have aroused much attention in the PDDS field.…”