Controlled Protein Delivery Based on Enzyme‐Responsive Nanocapsules

Wen, Jing; Anderson, Seth C.; Du, Juanjuan; Yan, Ming; Wang, Jun; Shen, Meiqing; Lu, Yunfeng; Segura, Tatiana

doi:10.1002/adma.201101771

Cited by 103 publications

(80 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Utilizing this nanoplatform, the researcher delivered both cytosolic and nuclear proteins, such as EGFP, CP3, BSA and the transcription factor Klf4, in active forms to different of cell lines. Wen et al reported an enzyme-responsive delivery platform with controlled-releasing capability and specificity based on protein nanocapsules [56]. When exposed to proteases, the peptide crosslinkers surrounding the protein surface were cleaved, releasing their protein cargo.…”

Section: Physiological Stimuli-triggered Deliverymentioning

confidence: 99%

Stimuli-responsive nanomaterials for therapeutic protein delivery

Lü

Sun

2014

Journal of Controlled Release

370

285

View full text Add to dashboard Cite

Protein therapeutics have emerged as a significant role in treatment of a broad spectrum of diseases, including cancer, metabolic disorders and autoimmune diseases. The efficacy of protein therapeutics, however, is limited by their instability, immunogenicity and short half-life. In order to overcome these barriers, tremendous efforts have recently been made in developing controlled protein delivery systems. Stimuli-triggered release is an appealing and promising approach for protein delivery and has made protein delivery with both spatiotemporal- and dosage-controlled manners possible. This review surveys recent advances in controlled protein delivery of proteins or peptides using stimuli-responsive nanomaterials. Strategies utilizing both physiological and external stimuli are introduced and discussed.

show abstract

Section: Physiological Stimuli-triggered Deliverymentioning

confidence: 99%

Stimuli-responsive nanomaterials for therapeutic protein delivery

Lü

Sun

2014

Journal of Controlled Release

370

285

View full text Add to dashboard Cite

show abstract

“…In our view, a particularly important advance in this area was reported recently for a novel encapsulation class (Wen et al, 2011). Test protein cargos were encapsulated in crosslinked aminopropyl-methacrylamide/acrylamide polymer-based nanocapsules (Wen et al, 2011)). Carriers incorporating plasmin-cleavable, or matrix metalloproteinase-cleavable, crosslinkers were tested at varying target peptide sequence contents.…”

Section: Food Pathogen-responsive Delivery Of Bioactivesmentioning

confidence: 93%

“…Carriers incorporating plasmin-cleavable, or matrix metalloproteinase-cleavable, crosslinkers were tested at varying target peptide sequence contents. Not only were encapsulated protein cargos released based upon the high specificity of the respective cross-linkers, but their rates of release were proportional to the relative crosslinker contents (Wen et al, 2011). This new class of nanocapsule for controlled, targeted delivery has not been proposed for application in food systems, to the best of our knowledge.…”

Section: Food Pathogen-responsive Delivery Of Bioactivesmentioning

confidence: 97%

“…If such disease-associated enzymes that are secreted can be co-opted for governing the controlled release of a bioactive cargo, based on specific cellular events or environmental changes, then such a scheme could be highly effective in terms of precise spatial and temporal control (Wen et al, 2011). In our view, a particularly important advance in this area was reported recently for a novel encapsulation class (Wen et al, 2011). Test protein cargos were encapsulated in crosslinked aminopropyl-methacrylamide/acrylamide polymer-based nanocapsules (Wen et al, 2011)).…”

Section: Food Pathogen-responsive Delivery Of Bioactivesmentioning

confidence: 99%

See 1 more Smart Citation

Nanotechnology: the word is new but the concept is old. An overview of the science and technology in food and food products at the nanoscale level

Bryksa

Yada

2012

Int. J. Food Stud.

View full text Add to dashboard Cite

Food scientists and technologists are actively engaged in examining and developing nanotechnologies for applications such as novel functional ingredients and nutrient delivery systems, safety testing, packaging, and authenticity/authentication at an ever-increasing pace. However, before these new products/technologies are commercialised, rigorous safety testing and risk/benefit analysis are required to ensure that public and environmental concerns are addressed. This review provides an overview of food nanoscience and technology including a brief history, education, definitions pertaining to policy and regulation, and applications. The most recent findings and advances are emphasised, focussing on bioactives' delivery. In addition, proposed directions in the area of nano-based targeting of pathogens for food safety as well as medical foods are discussed. As food nanoscience and technology has been extenisvely reviewed in recent years, specific case examples will be limited to those reported within the past year.

show abstract

“…The nano-layer protects the therapeutic protein against the degradation by proteases in the systemic circulation [34]. Furthermore, responsive polymer layers can releases its protein cargoes in the cell under specific local intracellular conditions or events, e.g., (1) at low pH values in endosomes (pH~5.5) [34] or (2) enzyme digestion [35]. The biocompatibility of haemoglobin nanocapsules covered by acrylamide-bisacrylamide random copolymer was investigated and good values were detected [36].…”

Section: Introductionmentioning

confidence: 99%

Biomedical Applications of Single Protein Nanoparticles

Hegedüs¹,

Kálmán²,

Faragó³

et al. 2014

JPP

View full text Add to dashboard Cite

Abstract:The drug carrier function of single protein nanoparticles, i.e., each individual protein molecule covered by a very thin, porous and few nanometer thick polymer layer, has been investigated. This layer around protein molecule is very thin, about 3-5 nm thick and highly porous, thus it does not reduce seriously the enzymatic function of protein molecule. The spatial structure of encapsulated protein molecule, which is essential in its function, can be stabilized by this polymer layer. Bovine serum albumin was used as protein drug molecule and it was encapsulated with acrylamide-bisarylamide random copolymer. The polymerization, starting from the modified sites of the surface of bovine serum albumin molecules was initiated by TEMED (tetramethylethylenediamine). These single albumin nanoparticles were painted with fluorescein isothiocyanate. This material was then injected into the inferior vena cava of rats. The treated rats were decapitated after 1 to 10 minutes and its brain was investigated by fluorescent microscopy. It was proved that bovine serum albumin molecules as drugs encapsulated in polymer nano-layer with a reduced size (about 10 nm) can pass through the blood brain barrier. The results suggest that this method is capable of transformation of biomacromolecules to access the brain tissue via the blood.

show abstract

Controlled Protein Delivery Based on Enzyme‐Responsive Nanocapsules

Cited by 103 publications

References 26 publications

Stimuli-responsive nanomaterials for therapeutic protein delivery

Stimuli-responsive nanomaterials for therapeutic protein delivery

Nanotechnology: the word is new but the concept is old. An overview of the science and technology in food and food products at the nanoscale level

Biomedical Applications of Single Protein Nanoparticles

Contact Info

Product

Resources

About