Controlled patterning of biomolecules on solid surfaces

Martelé, Yves; Callewaert, Kristof; Naessens, Kris; Daele, Peter Van; Baets, Roel; Schacht, Etienne

doi:10.1016/s0928-4931(02)00281-3

Cited by 14 publications

(4 citation statements)

References 7 publications

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“…Numerous biosensor interfacial systems are based on specific recognition. Several well-known examples include biotin−streptavidin or avidin recognition, − carbohydrate−protein recognition, − nitrolotriacetic acid−histidine-tagged protein recognition, − cholera toxin−ganglioside recognition, benzenesulfonamide−carbonic anhydrase, glucoseoxidase-based electrode sensors, − and antibody−antigen interaction in immunosensors. − These types of SAM-based biosensors, where the antigen (or ligand) or protein becomes immobilized on the transducer surface, can be detected using optical, electrochemical, and acoustic signal transduction mechanisms among others depending on the amount, environment, and response time. Several reviews have discussed some of the SAM applications as biosensing devices in detail. , …”

Section: Surface Interactions With Proteins and Cellsmentioning

confidence: 99%

Self-Assembled Monolayers and Polymer Brushes in Biotechnology: Current Applications and Future Perspectives

2005

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The chemistry and topography of a surface affect biological response and are of fundamental importance, especially when living systems encounter synthetic surfaces. Most biomolecules have immense recognition power (specific binding) and simultaneously have a tendency to physically adsorb onto a solid substrate without specific receptor recognition (nonspecific adsorption). Therefore, to create useful materials for many biotechnology applications, interfaces are required that have both enhanced specific binding and reduced nonspecific binding. Thus, in applications such as sensors, the tailoring of surface chemistry and the use of micro or nanofabrication techniques becomes an important avenue for the production of surfaces with specific binding properties and minimal background interference. Both self-assembled monolayers (SAMs) and polymer brushes have attracted considerable attention as surface-active materials. In this review, we discuss both of these materials with their potential applications in biotechnology. We also summarize lithographic methods for pattern formation using combined top-down and bottom-up approaches and briefly discuss the future of these materials by describing emerging new applications.

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Section: Surface Interactions With Proteins and Cellsmentioning

confidence: 99%

Self-Assembled Monolayers and Polymer Brushes in Biotechnology: Current Applications and Future Perspectives

2005

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“…Furthermore, thin metal coatings could also be used as starting layers for self-assembled monolayers, SAMs, e.g. to control cell adhesion (Chen et al, 1998;Martelé et al, 2003;Mrksich and Whitesides, 1996). The mask based sputtering process with silver onto the plane films lead to a pattern of 25 × 25 μm 2 sized quadratic spots with a thickness of about 100 nm.…”

Section: Durability and Distortion Of Surface Featuresmentioning

confidence: 99%

3D tissue culture substrates produced by microthermoforming of pre-processed polymer films

Giselbrecht

Gietzelt

Gottwald

et al. 2006

Biomed Microdevices

107

108

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We describe a new technology based on thermoforming as a microfabrication process. It significantly enhances the tailoring of polymers for three dimensional tissue engineering purposes since for the first time highly resolved surface and bulk modifications prior to a microstructuring process can be realised. In contrast to typical micro moulding techniques, the melting phase is avoided and thus allows the forming of pre-processed polymer films. The polymer is formed in a thermoelastic state without loss of material coherence. Therefore, previously generated modifications can be preserved. To prove the feasibility of our newly developed technique, so called SMART = Substrate Modification And Replication by Thermoforming, polymer films treated by various polymer modification methods, like UV-based patterned films, and films modified by the bombardment with energetic heavy ions, were post-processed by microthermoforming. The preservation of locally applied specific surface and bulk features was demonstrated e.g. by the selective adhesion of cells to patterned microcavity walls.

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“…Spatial localization of a redox enzyme on an electrode is of crucial importance in the prospective development of an enzymatic electrochemical platform that can be applied to green and sustainable electrical or biochemical energy harvesting systems. [ 1–5 ] In nature, redox enzymes are organized in the membrane matrix with controlled spacing and positions to accomplish enzymatic catalysis with high catalytic efficiency. [ 6,7 ] Specifically, such a spatial organization of enzymes enables the efficient delivery of metabolites among enzyme active sites while preventing the steric hindrance of a reaction site by adjacent proteins.…”

Section: Introductionmentioning

confidence: 99%

Versatile Biosynthetic Approach to Regioselective Enzyme Patterning for Direct Bioelectrocatalysis Application

Lee

Reginald

Chang

2023

Adv Materials Technologies

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accomplish enzymatic catalysis with high catalytic efficiency. [6,7] Specifically, such a spatial organization of enzymes enables the efficient delivery of metabolites among enzyme active sites while preventing the steric hindrance of a reaction site by adjacent proteins. [8] Therefore, it is essential to consider the effect of enzyme spatial organization on overall metabolic efficiency for translating our knowledge about natural biochemical systems into noncellular artificial environments, such as those involving electrodes. [9] Inspired by nature, a generation of proper multior single enzymatic positioning on electrodes can bring about enhanced performance of enzyme-based bioelectrocatalysis systems. [10] Regarding in vitro spatial organization, there have been considerable efforts to achieve technical advances in position-specific enzyme immobilization by performing "protein patterning". [11,12] Protein molecules have been patterned using direct or indirect assembly (i.e., self-assembled monolayers alkanethiol, silane, Ni-NTA, biotin, etc.) by integration with lithographic approaches including scanning probe microscopy, [13,14] dip-pen nanolithography (DPN), [15] atomic force microscopy (AFM), [16] electron beam (e-beam) lithography (EBL), [17] and nanoimprint lithography (NIL). [18] DNA templates have also been widely used to create periodic arrays of protein molecules. [19] Yan et al. (2003) reported that DNA tiles with sticky ends self-assembled into nanoribbons or nanogrids that provided a predetermined surface for target-molecule binding. [20] Since the enzyme-based electrocatalysis system operates cooperatively by single-or multienzyme-based catalysis and electron transfer (ET) between the enzymatic cofactor and electrode, electrical communication of the enzyme-electrode should also be considered and controlled together with enzymatic surface-positioning. [21,22] However, most of the previous studies involving the development of protein micro/nanopatterning techniques were not primarily concerned with the enzymeelectrode interface. The approaches for protein patterning have not proven to be amenable in controlling the orientations of enzymes on surfaces. Hence, extensive chemical/biochemical surface modification or random attachments of the protein may electrically block the cofactor-surface interfaces, limiting the interfacial efficiency of direct ET (DET). [23] Due to the outstanding attributes of oxidoreductases, they have been utilized as biomaterials for bioelectrocatalytic systems. Herein, a simple and versatile biosynthetic approach that can designate binding position of enzymes on electrode with their surface-orientation is suggested. In this regard, materialselective properties of gold-binding peptide (GBP) are exploited and genetically fused GBP to enzyme. To optimize the design of synthetic enzyme, a variable repeat number of GBP are fused to flavin adenine dinucleotidedependent glucose dehydrogenase gamma-alpha complex (GDHγα) and their catalytic and gold-binding activities are determined. T...

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Controlled patterning of biomolecules on solid surfaces

Cited by 14 publications

References 7 publications

Self-Assembled Monolayers and Polymer Brushes in Biotechnology: Current Applications and Future Perspectives

Self-Assembled Monolayers and Polymer Brushes in Biotechnology: Current Applications and Future Perspectives

3D tissue culture substrates produced by microthermoforming of pre-processed polymer films

Versatile Biosynthetic Approach to Regioselective Enzyme Patterning for Direct Bioelectrocatalysis Application

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