Controlled nucleation of hydroxyapatite on alginate scaffolds for stem cell‐based bone tissue engineering

Suárez-González, Darilis; Barnhart, Kara; Saito, Eiji; Vanderby, Ray; Hollister, Scott J.; Murphy, William L.

doi:10.1002/jbm.a.32833

Cited by 113 publications

(94 citation statements)

References 46 publications

(101 reference statements)

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“…The coating displayed a nanoporous structure in electron micrographs (Fig. 1a-c) similar to prior studies from our group [31][32][33][34] and its ratio of calcium and phosphorus was 1.67 -0.03 (inset of Fig. 1c).…”

Section: Cap Mineral Coating Characteristicssupporting

confidence: 85%

Effects of BMP-12-Releasing Sutures on Achilles Tendon Healing

Chamberlain

Lee

Leiferman

et al. 2015

Tissue Engineering Part A

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Tendon healing is a complex coordinated event orchestrated by numerous biologically active proteins. Unfortunately, tendons have limited regenerative potential and as a result, repair may be protracted months to years. Current treatment strategies do not offer localized delivery of biologically active proteins, which may result in reduced therapeutic efficacy. Surgical sutures coated with nanostructured minerals may provide a potentially universal tool to efficiently incorporate and deliver biologically active proteins directly to the wound. Additionally, previous reports indicated that treatment with bone morphogenetic protein-12 (BMP-12) improved tendon healing. Based on this information, we hypothesized that mineral-coated surgical sutures may be an effective platform for localized BMP-12 delivery to an injured tendon. The objective of this study was, therefore, to elucidate the healing effects of mineral-coated sutures releasing BMP-12 using a rat Achilles healing model. The effects of BMP-12-releasing sutures were also compared with standard BMP-12 delivery methods, including delivery of BMP-12 through collagen sponge or direct injection. Rat Achilles tendons were unilaterally transected and repaired using BMP-12-releasing suture (0, 0.15, 1.5, or 3.0 mg), collagen sponge (0 or 1.5 mg BMP-12), or direct injection (0 or 1.5 mg). By 14 days postinjury, repair with BMP-12-releasing sutures reduced the appearance of adhesions to the tendon and decreased total cell numbers. BMP-12 released from sutures and collagen sponge also tended to improve collagen organization when compared with BMP-12 delivered through injection. Based on these results, the release of a protein from sutures was able to elicit a biological response. Furthermore, BMP-12-releasing sutures modulated tendon healing, and the delivery method dictated the response of the healing tissue to BMP-12.

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Section: Cap Mineral Coating Characteristicssupporting

confidence: 85%

Effects of BMP-12-Releasing Sutures on Achilles Tendon Healing

Chamberlain

Lee

Leiferman

et al. 2015

Tissue Engineering Part A

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“…In composite materials, HA or tricalcium phosphate is combined with a variety of monomeric and polymeric materials such as silica, 8 alginate, 9 polycaprolactone, 10 poly(lactic-co-glycolic acid), 11 poly-L-lactide, 12 polymethylmethacrylate, 13 gelatin and silk fibroin, 14 poly(1,8-octanediol-co-citrate), 15 collagen, chitosan, or synthetic peptide with a β-sheet structure. 16 This combination of polymers and ceramics in the form of composite materials can overcome the drawbacks due to their inappropriate mechanical properties for load-bearing applications.…”

Section: Introductionmentioning

confidence: 99%

Support for the initial attachment, growth and differentiation of MG-63 cells: a comparison between nano-size hydroxyapatite and micro-size hydroxyapatite in composites

Filová¹,

Suchý²,

Sucharda³

et al. 2014

IJN

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Hydroxyapatite (HA) is considered to be a bioactive material that favorably influences the adhesion, growth, and osteogenic differentiation of osteoblasts. To optimize the cell response on the hydroxyapatite composite, it is desirable to assess the optimum concentration and also the optimum particle size. The aim of our study was to prepare composite materials made of polydimethylsiloxane, polyamide, and nano-sized (N) or micro-sized (M) HA, with an HA content of 0%, 2%, 5%, 10%, 15%, 20%, 25% (v/v) (referred to as N0–N25 or M0–M25), and to evaluate them in vitro in cultures with human osteoblast-like MG-63 cells. For clinical applications, fast osseointegration of the implant into the bone is essential. We observed the greatest initial cell adhesion on composites M10 and N5. Nano-sized HA supported cell growth, especially during the first 3 days of culture. On composites with micro-size HA (2%–15%), MG-63 cells reached the highest densities on day 7. Samples M20 and M25, however, were toxic for MG-63 cells, although these composites supported the production of osteocalcin in these cells. On N2, a higher concentration of osteopontin was found in MG-63 cells. For biomedical applications, the concentration range of 5%–15% (v/v) nano-size or micro-size HA seems to be optimum.

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“…Alginate is a naturally derived polymer, which has been wildly employed in dispensing-based biofabrication for various applications such as nerve tissue engineering [5,6] and bone tissue engineering [7,8], because of its good biocompatibility and ease gelation in the presence of calcium ions [5,9,10]. And calcium ions have been used to crosslink alginate in order to form hydrogel in dispensing-based biofabrication process.…”

Section: Introductionmentioning

confidence: 99%

Influence of Calcium Ions on Cell Survival and Proliferation in the Context of an Alginate Hydrogel

Cao

Chen

Schreyer

2012

ISRN Chemical Engineering

107

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One goal of biofabrication is to incorporate living cells into artificial scaffolds in order to repair damaged tissues or organs. Although there are many studies on various biofabrication techniques, the maintenance of cell viability during the biofabrication process and cell proliferation after the process is still a challenging issue. Construction of scaffolds using hydrogels composed of natural materials can avoid exposure of cells to harsh chemicals or temperature extremes but can still entail exposure to nonphysiological conditions, causing cell damage or even death. This paper presents an experimental investigation into the influence on Schwann cell survival and proliferation of calcium used for ionic crosslinking of alginate hydrogel during the biofabrication process. The experimental results obtained show the viability and proliferation capacity of cells, either suspended in cell culture medium or encapsulated in hydrogel, and vary with the calcium concentration and the time period of cells exposed to the calcium environment. The experimental results also show the alginate concentration and cell density, that have profound influence on cell survival and proliferation, and solution viscosity as well. This study suggests the incorporation of living cells in calcium-crosslinked hydrogel in the biofabrication process can be regulated for controlled cell survival and proliferation.

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Controlled nucleation of hydroxyapatite on alginate scaffolds for stem cell‐based bone tissue engineering

Cited by 113 publications

References 46 publications

Effects of BMP-12-Releasing Sutures on Achilles Tendon Healing

Effects of BMP-12-Releasing Sutures on Achilles Tendon Healing

Support for the initial attachment, growth and differentiation of MG-63 cells: a comparison between nano-size hydroxyapatite and micro-size hydroxyapatite in composites

Influence of Calcium Ions on Cell Survival and Proliferation in the Context of an Alginate Hydrogel

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