Controlled nerve growth factor release from multi-ply alginate/chitosan-based nerve conduits

Pfister, Lukas A.; Alther, Eva; Papaloizos, Michael L.; Merkle, Hans P.; Gander, Bruno

doi:10.1016/j.ejpb.2008.01.014

Cited by 61 publications

(50 citation statements)

References 25 publications

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“…Alginate is a naturally derived polymer, which has been wildly employed in dispensing-based biofabrication for various applications such as nerve tissue engineering [5,6] and bone tissue engineering [7,8], because of its good biocompatibility and ease gelation in the presence of calcium ions [5,9,10]. And calcium ions have been used to crosslink alginate in order to form hydrogel in dispensing-based biofabrication process.…”

Section: Introductionmentioning

confidence: 99%

Influence of Calcium Ions on Cell Survival and Proliferation in the Context of an Alginate Hydrogel

Cao

Chen

Schreyer

2012

ISRN Chemical Engineering

111

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One goal of biofabrication is to incorporate living cells into artificial scaffolds in order to repair damaged tissues or organs. Although there are many studies on various biofabrication techniques, the maintenance of cell viability during the biofabrication process and cell proliferation after the process is still a challenging issue. Construction of scaffolds using hydrogels composed of natural materials can avoid exposure of cells to harsh chemicals or temperature extremes but can still entail exposure to nonphysiological conditions, causing cell damage or even death. This paper presents an experimental investigation into the influence on Schwann cell survival and proliferation of calcium used for ionic crosslinking of alginate hydrogel during the biofabrication process. The experimental results obtained show the viability and proliferation capacity of cells, either suspended in cell culture medium or encapsulated in hydrogel, and vary with the calcium concentration and the time period of cells exposed to the calcium environment. The experimental results also show the alginate concentration and cell density, that have profound influence on cell survival and proliferation, and solution viscosity as well. This study suggests the incorporation of living cells in calcium-crosslinked hydrogel in the biofabrication process can be regulated for controlled cell survival and proliferation.

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Section: Introductionmentioning

confidence: 99%

Influence of Calcium Ions on Cell Survival and Proliferation in the Context of an Alginate Hydrogel

Cao

Chen

Schreyer

2012

ISRN Chemical Engineering

111

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“…The release profile of growth factors from biomaterial devices has been monitored in the range of hours, 24 days, 26 and weeks; 10 however, the 13 days duration limit of testing was selected based on the instability of NGF upon extended incubation times 31 Although not investigated in this work, embedding of NGFloaded spheres within collagen hydrogel can further slowdown protein release. 32 Therefore, growth factor-loaded spheres may be directly injected into injury site or, as illustrated in Scheme 1, spheres can be embedded within hydrogel and then placed directly into a lesion site in injured spinal cord.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…27 NGF Release from Spheres. NGF in vitro release profile was investigated in a PC12 cell system as well as in an acellular system where 0.05% Tween 20−PBS, which improves NGF stability, 26 was used as a release buffer. Each of the three types of collagen hollow sphere showed an ability to slowly release NGF in both the cells and no-cells systems.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Assembly of Protein-Based Hollow Spheres Encapsulating a Therapeutic Factor

Kraskiewicz

Breen²,

Sargeant³

et al. 2013

ACS Chem. Neurosci.

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Neurotrophins, as important regulators of neural development, function, and survival, have a therapeutic potential to repair damaged neurons. However, a controlled delivery of therapeutic molecules to injured tissue remains one of the greatest challenges facing the translation of novel drug therapeutics field. This study presents the development of an innovative protein−protein delivery technology of nerve growth factor (NGF) by an electrostatically assembled protein-based (collagen) reservoir system that can be directly injected into the injury site and provide long-term release of the therapeutic. A protein-based biomimetic hollow reservoir system was fabricated using a template method. The capability of neurotrophins to localize in these reservoir systems was confirmed by confocal images of fluorescently labeled collagen and NGF. In addition, high loading efficiency of the reservoir system was proven using ELISA. By comparing release profile from microspheres with varying cross-linking, highly cross-linked collagen spheres were chosen as they have the slowest release rate. Finally, biological activity of released NGF was assessed using rat pheochromocytoma (PC12) cell line and primary rat dorsal root ganglion (DRG) cell bioassay where cell treatment with NGF-loaded reservoirs induced significant neuronal outgrowth, similar to that seen in NGF treated controls. Data presented here highlights the potential of a high capacity reservoir-growth factor technology as a promising therapeutic treatment for neuroregenerative applications and other neurodegenerative diseases.

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“…Along with the development of tissue engineering, scientists have shown that implanting nerve guide conduits containing Schwann cells can promote the regeneration of the nerve axons [5] . In the other researches, NGF is found necessary to support the survival of the cell bodies and the regeneration of the axons toward specific target organs [6][7][8][9] . Although NGF has been shown to improve nerve regeneration under certain conditions, diffi culties in manufacture of these large proteins and delivery to target sites are big barriers to therapeutic application [10] .…”

Section: Introductionmentioning

confidence: 99%

In vitro biocompatibility assessment of a novel PRGD/PDLLA/NGF composite material

Yin

Yan

et al. 2011

J. Wuhan Univ. Technol.-Mat. Sci. Edit.

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The objective of this study was to evaluate the degradability and biocompatibility of a novel composite materials which was grafted with RGD and immobilized with NGF(PRGD/PDLLA/NGF). The releasing of NGF, the biodegradability and cell-biocompatibility of PRGD/PDLLA/NGF membrane were evaluated in vitro. The experimental results showed that the NGF release process was prolonged over 30 days. Furthermore, the PRGD/PDLLA/NGF showed a better hydrophilicity, better biodegradation properties and cells affi nity than PDLLA, which means a good support to adhesion and proliferate of Schwann cells. Therefore, the novel composite material holds considerable promise as scaffolds in nerve tissue engineering.

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Controlled nerve growth factor release from multi-ply alginate/chitosan-based nerve conduits

Cited by 61 publications

References 25 publications

Influence of Calcium Ions on Cell Survival and Proliferation in the Context of an Alginate Hydrogel

Influence of Calcium Ions on Cell Survival and Proliferation in the Context of an Alginate Hydrogel

Assembly of Protein-Based Hollow Spheres Encapsulating a Therapeutic Factor

In vitro biocompatibility assessment of a novel PRGD/PDLLA/NGF composite material

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