Controlled microwave derivatization reaction for reproducible trace analysis of budesonide in human plasma

Derivatization procedures and their analytical performances for HPLC determination in bioanalysis

“…Derivatization efficiency and kinetics can be improved by applying a physical treatment. For example, microwave‐assisted derivatization or ultrasound‐assisted derivatization are common approaches for shortening the reaction time (Ahmed & Atia, 2019). Microwave‐assisted derivatization was used for the HPLC–fluorescence analysis of nitrofuran metabolites in shrimp samples (Luo et al, 2019), using a novel derivatization reagent, 4‐(acridone‐10‐yl)benzaldehyde.…”

A simple and rapid HPLC-UV method for the determination of valproic acid in human plasma using microwave-assisted derivatization with phenylhydrazine hydrochloride

“…Several analytical methods, including liquid chromatography-mass spectrometry (LC-MS) [ 5 , 6 ], gas chromatography-mass spectrometry (GC-MS) [ 7 , 8 ], and immunoassays [ 9 ], have been developed for the quantification of VPA. Advanced analytical methods such as LC-MS and GC-MS require highly skilled operators and are expensive for sample analysis [ 10 ]. The GC-MS method for determining VPA involved pre-column esterification, utilizing methanol as the derivatizing agent and the sample was heated at 60 °C for 30 min.…”