Controlled Insulin Release from Chitosan Microparticles

Bugamelli, Francesca; Raggi, Maria Augusta; Orienti, Isabella; Zecchi, Vittorio

doi:10.1002/(sici)1521-4184(199804)331:4<133::aid-ardp133>3.0.co;2-h

Cited by 40 publications

(9 citation statements)

References 16 publications

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“…There are several published methods for manufacturing drug or protein-loaded chitosan particles [15-18]. Methods include the use of crosslinking agents [16, 22, 23], emulsion-precipitation [24], ionotropic gelation and precipitation-coacervation.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, there exists a need to develop a methodology for effective control of chitosan particle size, protein loading efficiency and protein release. Chitosan particles have been manufactured via a variety of chemistries including cross-linking, ionotropic gelation and precipitation-coacervation [15-18]. These last two methods are particularly attractive for the delivery of labile polypeptides as encapsulation is accomplished under mild aqueous conditions.…”

Section: Introductionmentioning

confidence: 99%

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Controlling chitosan-based encapsulation for protein and vaccine delivery

et al. 2014

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Chitosan-based nano/microencapsulation is under increasing investigation for the delivery of drugs, biologics and vaccines. Despite widespread interest, the literature lacks a defined methodology to control chitosan particle size and drug/protein release kinetics. In this study, the effects of precipitation-coacervation formulation parameters on chitosan particle size, protein encapsulation efficiency and protein release were investigated. Chitosan particle sizes, which ranged from 300 nm to 3 μm, were influenced by chitosan concentration, chitosan molecular weight and addition rate of precipitant salt. The composition of precipitant salt played a significant role in particle formation with upper Hofmeister series salts containing strongly hydrated anions yielding particles with a low polydispersity index (PDI) while weaker anions resulted in aggregated particles with high PDIs. Sonication power had minimal effect on mean particle size, however, it significantly reduced polydispersity. Protein loading efficiencies in chitosan nano/microparticles, which ranged from 14.3% to 99.2%, was inversely related to the hydration strength of precipitant salts, protein molecular weight and directly related to the concentration and molecular weight of chitosan. Protein release rates increased with particle size and were generally inversely related to protein molecular weight. This study demonstrates that chitosan nano/microparticles with high protein loading efficiencies can be engineered with well-defined sizes and controllable release kinetics through manipulation of specific formulation parameters.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Controlling chitosan-based encapsulation for protein and vaccine delivery

et al. 2014

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“…The potentiation of the antimetastatic effect of a peptide by hybrid with a polymer was proposed to be based on slower enzymatic degradation and more stable receptor binding of the hybrid as compared with the parent peptide. Bugamelli et al (1998) prepared and evaluated chitosan microspheres containing insulin. A constant release of the peptide for a period in excess of 80 h was observed in-vitro.…”

Section: Controlled Drug Deliverymentioning

confidence: 99%

Chitosan: some pharmaceutical and biological aspects - an update

Singla

Chawla

2001

Journal of Pharmacy and Pharmacology

699

363

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Chitosan, a natural polysaccharide, is being widely used as a pharmaceutical excipient. It is obtained by the partial deacetylation of chitin, the second most abundant natural polymer.Chitosan comprises a series of polymers varying in their degree of deacetylation, molecular weight, viscosity, pKa etc. The presence of a number of amino groups permit chitosan to chemically react with anionic systems, thereby resulting in alteration of physicochemical characteristics of such combinations.Chitosan has found wide applicability in conventional pharmaceutical devices as a potential formulation excipient, some of which include binding, disintegrating and tablet coating properties. The polymer has also been investigated as a potential adjuvant for swellable controlled drug delivery systems. Use of chitosan in novel drug delivery as mucoadhesive, gene and peptide drug administration via the oral route as well as its absorption enhancing effects have been explored by a number of researchers.Chitosan exhibits myriad biological actions, namely hypocholesterolemic, antimicrobial and wound healing properties. Low toxicity coupled with wide applicability makes it a promising candidate not only for the purpose of drug delivery for a host of drug moieties (antiinflammatories, peptides etc.) but also as a biologically active agent.It is the endeavour of the present review to provide an insight into the biological and pharmaceutical profile of chitosan. Various investigations carried out recently are reported, although references to research performed on chitosan prior to the recent reviews have also been included, where appropriate.

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“…Chitosan was prepared by alkaline deacetylation of chitin (14)(15)(16) as follow: In a round bottle, 5 g of chitin and 500 ml NaOH (40% w/v) was boiled for 5 hours at 120 o C. Reflex apparatus was connected to the neck of the bottle to keep the volume of NaOH constant and to prevent evaporation. After 5 hours the mixture was left to cool at room temperature, and then filtered.…”

Section: Preparation Of Chitosanmentioning

confidence: 99%

Locally Made Chitosan Covalently Linked to Indomethacin: A Novel Approach to Formulate Sustained Release Indomethacin Capsule in Basrah

Thajeel¹,

Ahmed²

2008

MJBU

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The sustained release capsules are pharmaceutical dosage form used for the treatment of chronic rather than acute diseases and are useful because of its convenience and less side effects. This work describes an attempt to formulate indomethacin sustained release capsule using indomethacin covalently linked to chitosan. Chitosan was prepared by alkaline-N-deacetylation of chitin, which was isolated from the shells of local shrimps. The yield of chitosan was found to be 80%. This polymer was found to be white crystal, odorless and the IR spectroscopy of it was similar to a standard reference. Chitosan has been covalently linked to indomethacin and the ratio of drug to polymer was found to be 5:1. The chemical interaction between the drug and the polymer was proved by IR studies. In vitro release at different pH were performed and the release of indomethacin in the prepared sustained release capsule were tested and compared with conventional indomethacin capsule INDOMIN-25mg, and a commercial sustained release capsule INDO-75-SR. The release of indomethacin in our product was completed within 12 hours, while the conventional INDOMIN capsule and commercial INDO-75-SR capsule released within 2.5 and 5 hours respectively. In conclusion a cheap, locally made chitosan can be used in formulation of sustained release capsules by covalently linked to drugs containing carboxyl group (like indomethacin) or can be modified to have a carboxyl group.

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Controlled Insulin Release from Chitosan Microparticles

Cited by 40 publications

References 16 publications

Controlling chitosan-based encapsulation for protein and vaccine delivery

Controlling chitosan-based encapsulation for protein and vaccine delivery

Chitosan: some pharmaceutical and biological aspects - an update

Locally Made Chitosan Covalently Linked to Indomethacin: A Novel Approach to Formulate Sustained Release Indomethacin Capsule in Basrah

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