Controlled induction of denitrification in Pseudomonas aureofaciens: A simplified denitrifier method for dual isotope analysis in NO3−

Zhu, Jing; Yu, Longfei; Bakken, Lars R.; Mørkved, Pål Tore; Mulder, Jan; Dörsch, Peter

doi:10.1016/j.scitotenv.2018.03.236

Cited by 14 publications

(31 citation statements)

References 47 publications

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“…The same frames were used as a base for NO collectors, in which NO was oxidized to NO 2 by CrO 3 , which was trapped subsequently as NO 3 − in a H 2 O 2 /NaOH solution. After removing excess H 2 O 2 with manganese dioxide (MnO 2 ) and neutralizing the trapping solution with hydrogen chloride (HCl), NO 3 − in the trapping solution was converted into N 2 O using a modified denitrifier method; the 15 N abundance was measured using a PreCon‐gas chromatography/isotope ratio mass spectrometry (GC/IRMS) system (ThermoFinnigan, Bremen, Germany). A detailed description of the field trapping method is given in the supporting information (Chapter 2; Figure S2, supporting information) together with the methodology and computational procedures (Chapter 1).…”

Section: Methodsmentioning

confidence: 99%

“…Extracts were frozen and analyzed later for NH 4 + and NO 3 − concentration and 15 N abundance. The atom% 15 N of NO 3 − in the KCl extracts was determined using the modified denitrifier method . The atom% 15 N in NH 4 + was determined after its quantitative conversion into N 2 O using a chemical method in which NH 4 + is oxidized to NO 2 − by hypobromite (BrO − ), followed by reduction of NO 2 − to N 2 O using azoimide (HN 3 ).…”

Section: Methodsmentioning

confidence: 99%

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Nitrification is the primary source for NO in N‐saturated subtropical forest soils: Results from in situ¹⁵N labeling

Kang

Dörsch

et al. 2020

Rapid Comm Mass Spectrometry

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Rationale Acidic subtropical forest soils that receive high atmospheric nitrogen (N) deposition have been identified as important sources of nitric oxide (NO). The relative importance of major processes producing NO is unclear. Methods To partition NO sources, we conducted an in situ tracing experiment with 15NH4NO3 and NH415NO3 in well‐drained acid soils of an N‐saturated subtropical forest in Chongqing, southwest China. Results In the 15NH4NO3 treatment, the 15N signature of NO emitted from the foot of the hillslope (Lower site) was similar to that of the NH4+ pool, indicating predominant autotrophic nitrification for NO formation. In the NH415NO3 treatment, the 15N enrichment of NO was smaller than that of the NO3− pool, suggesting minor contribution of denitrification to NO production (~15%). Conclusions Nitrification is the main process responsible for NO emissions, even in monsoonal summers when soil water‐filled pore space values are relatively high.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Nitrification is the primary source for NO in N‐saturated subtropical forest soils: Results from in situ¹⁵N labeling

Kang

Dörsch

et al. 2020

Rapid Comm Mass Spectrometry

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“…Natural abundance of 15 N and 18 O in NO 3 − (

δ^{15} N_{{NO}_{3}}

and

δ^{18} O_{{NO}_{3}}

) were analyzed using a modified denitrifier method converting NO 3 − to N 2 O (Yu et al, ; Zhu et al, ). Briefly, tryptic soya broth (TSB) was pretreated with Paracoccus denitrificans (ATCC 17741) to remove background NO 3 − , amended with NH 4 Cl, autoclaved and filtered, before inoculation with an overnight culture of Pseudomonas aureofaciens (ATCC 13985), a denitrifier lacking N 2 O reductase.…”

Section: Methodsmentioning

confidence: 99%

“…Analysis of δ 15 N and δ 18 O of N 2 O was done directly from the headspace using purge‐and‐trap isotope ratio mass spectrometry (PreCon‐GC‐IRMS, Thermo Finnigan MAT, Bremen, Germany) at the Norwegian University of Life Sciences. International standards (IAEA N3, USGS 32 and 34) were included in each batch for calibration and correction (Zhu et al, ). Overall repeatability of our measurements was 0.1‰–0.3‰ for δ 15 N and 0.2‰–0.7‰ for δ 18 O.…”

Section: Methodsmentioning

confidence: 99%

Denitrification as a major regional nitrogen sink in subtropical forest catchments: Evidence from multi‐site dual nitrate isotopes

Mulder

Zhu

et al. 2019

Global Change Biology

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Increasing nitrogen (N) deposition in subtropical forests in south China causes N saturation, associated with significant nitrate (NO3−) leaching. Strong N attenuation may occur in groundwater discharge zones hydrologically connected to well‐drained hillslopes, as has been shown for the subtropical headwater catchment “TieShanPing”, where dual NO3− isotopes indicated that groundwater discharge zones act as an important N sink and hotspot for denitrification. Here, we present a regional study reporting inorganic N fluxes over two years together with dual NO3− isotope signatures obtained in two summer campaigns from seven forested catchments in China, representing a gradient in climate and atmospheric N input. In all catchments, fluxes of dissolved inorganic N indicated efficient conversion of NH4+ to NO3− on well‐drained hillslopes, and subsequent interflow of NO3− over the argic B‐horizons to groundwater discharge zones. Depletion of 15N‐ and 18O–NO3− on hillslopes suggested nitrification as the main source of NO3−. In all catchments, except one of the northern sites, which had low N deposition rates, NO3− attenuation by denitrification occurred in groundwater discharge zones, as indicated by simultaneous 15N and 18O enrichment in residual NO3−. By contrast to the southern sites, the northern catchments lack continuous and well‐developed groundwater discharge zones, explaining less efficient N removal. Using a model based on 15NO3− signatures, we estimated denitrification fluxes from 2.4 to 21.7 kg N ha−1 year−1 for the southern sites, accounting for more than half of the observed N removal. Across the southern catchments, estimated denitrification scaled proportionally with N deposition. Together, this indicates that N removal by denitrification is an important component of the N budget of southern Chinese forests and that natural NO3− attenuation may increase with increasing N input, thus partly counteracting further aggravation of N contamination of surface waters in the region.

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“…Therefore, the rapid and complete transformation of nitrate into N 2 O should be considered in the selection and culture of bacteria. Zhu et al [44] modified the classical denitrifier method with P. aureofaciens for robust and reliable conversion of nitrate to N 2 O without prior induction of denitrification with extraneous nitrate.…”

Section: Research Progress In Analytical Techniques Of Nitrogen and Omentioning

confidence: 99%

Application of Nitrogen and Oxygen Isotopes for Source and Fate Identification of Nitrate Pollution in Surface Water: A Review

et al. 2018

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Nitrate pollution in surface water has become an environmental problem of global concern. The effective way for controlling the nitrate pollution of surface water is to identify the pollution source and reduce the input of nitrate. In recent decades, nitrogen (δ 15 N) and oxygen (δ 18 O) isotopes of nitrate has been used as an effective approach for identifying the source and fate of nitrate pollution in surface water. However, owing to the complexity of nitrate pollution source and the influence of isotopic fractionation, the application of this method has some limitations. In this work, we systematically discussed the fundamental principle of using nitrogen and oxygen isotopes to trace the nitrate source, the fate identification of nitrate, and the major testing techniques. Subsequently, the applications of nitrogen and oxygen isotopes for source identification of surface water were illustrated. However, there are still significant gaps in the application of the source identification and transformation mechanisms to nitrate and many research questions on these topics need to be addressed.

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Controlled induction of denitrification in Pseudomonas aureofaciens: A simplified denitrifier method for dual isotope analysis in NO3−

Cited by 14 publications

References 47 publications

Nitrification is the primary source for NO in N‐saturated subtropical forest soils: Results from in situ¹⁵N labeling

Nitrification is the primary source for NO in N‐saturated subtropical forest soils: Results from in situ¹⁵N labeling

Denitrification as a major regional nitrogen sink in subtropical forest catchments: Evidence from multi‐site dual nitrate isotopes

Application of Nitrogen and Oxygen Isotopes for Source and Fate Identification of Nitrate Pollution in Surface Water: A Review

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Controlled induction of denitrification in Pseudomonas aureofaciens: A simplified denitrifier method for dual isotope analysis in NO3−

Cited by 14 publications

References 47 publications

Nitrification is the primary source for NO in N‐saturated subtropical forest soils: Results from in situ15N labeling

Nitrification is the primary source for NO in N‐saturated subtropical forest soils: Results from in situ15N labeling

Denitrification as a major regional nitrogen sink in subtropical forest catchments: Evidence from multi‐site dual nitrate isotopes

Application of Nitrogen and Oxygen Isotopes for Source and Fate Identification of Nitrate Pollution in Surface Water: A Review

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Nitrification is the primary source for NO in N‐saturated subtropical forest soils: Results from in situ¹⁵N labeling

Nitrification is the primary source for NO in N‐saturated subtropical forest soils: Results from in situ¹⁵N labeling