Controlled ice nucleation in cryopreservation – A review

Morris, G.J.; Acton, Elizabeth

doi:10.1016/j.cryobiol.2012.11.007

Cited by 226 publications

(236 citation statements)

References 75 publications

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“…The IN-ESEM platform can also be applied to other research areas where well-controlled temperature or RH are required such as cryobiology. [1][2][3] The IN cell and associated control units are modular and can be implemented in other analytical instrumentation such as a Raman or Fourier transform infrared microscope to investigate the interaction of ice or water with other materials. 84 The sample holder can be readily modified to suit different samples/substrate sizes.…”

Section: Discussionmentioning

confidence: 99%

“…However, ice can also form on a substrate by heterogeneous nucleation at higher temperatures that lie between the homogeneous freezing limit and the ice melting point. In the applied sciences, the heterogeneous nucleation of ice plays an important role in technologies of cryopreservation, [1][2][3] freeze-drying in biomedical research and the food industry, 4 and the development of anti-icing coatings for aircraft. 5,6 In atmospheric chemistry and physics, ice nucleation initiated by airborne particles governs the formation and microphysical properties of ice and mixed phase clouds.…”

Section: Introductionmentioning

confidence: 99%

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Direct observation of ice nucleation events on individual atmospheric particles

Wang

Knopf

China

et al. 2016

Phys. Chem. Chem. Phys.

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The work presents microscopic observations of heterogeneous ice nucleation from experiments conducted inside an environmental scanning electron microscope. Observations of ice formation on kaolinite particles demonstrate that ice preferentially nucleates at the edges of the stacked platelets, rather than on the basal planes. This platform is applied for directly detecting and tracking ice nucleating particles in ambient aerosol samples and is complemented by micro-spectroscopic chemical imaging. This technique opens a path to new physical chemistry studies of ice formation in atmospheric science, cryobiology, and material science.www.rsc.org/pccp Heterogeneous ice nucleation is a physical chemistry process of critical relevance to a range of topics in the fundamental and applied sciences and technologies. Heterogeneous ice nucleation remains insufficiently understood, partially due to the lack of experimental methods capable of obtaining in situ microscopic details of ice formation over nucleating substrates or particles. We present microscopic observations of ice nucleation events on kaolinite particles at the nanoscale and demonstrate the capability of direct tracking and micro-spectroscopic characterization of individual ice nucleating particles (INPs) in an authentic atmospheric sample. This approach utilizes a custom-built ice nucleation cell, interfaced with an Environmental Scanning Electron Microscope (IN-ESEM platform) operated at temperatures and relative humidities relevant for heterogeneous ice nucleation. The IN-ESEM platform allows dynamic observations of individual ice formation events over particles in isobaric and isothermal experiments. Isothermal experiments on individual kaolinite particles demonstrate that ice crystals preferably nucleate at the edges of the stacked kaolinite platelets, rather than on their basal planes.These experimental observations of the location of ice nucleation provide direct information for further theoretical chemistry predictions of ice formation on kaolinite.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Direct observation of ice nucleation events on individual atmospheric particles

Wang

Knopf

China

et al. 2016

Phys. Chem. Chem. Phys.

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“…For stem cells cryopreserved as colony fragments, if slow cooling methods are to be applied, consideration should be given to methods to control ice nucleation such as the inclusion of ice nucleating agents or seeding samples at high sub-zero temperature [111].…”

Section: Appendix 8: Preservation Technologies and Methodsmentioning

confidence: 99%

Points to Consider in the Development of Seed Stocks of Pluripotent Stem Cells for Clinical Applications: International Stem Cell Banking Initiative (ISCBI)

et al. 2015

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“…The critical moment is when the temperature achieves values below 0°C, when there is formation of ice crystals, which is an under-recognized phenomenon because water nucleation is not well understood (Morris and Acton, 2013). When a large amount of water in the cell cytoplasm is subjected to a rapid temperature decrease to temperatures well below 0°C, immediate formation of ice crystals occurs in the entire cell volume containing water.…”

Section: Discussionmentioning

confidence: 99%

Protocol to cryopreserve and isolate nuclei from adipose tissue without dimethyl sulfoxide

et al. 2014

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ABSTRACT. Cryopreservation injuries involve nuclear DNA damage. A protocol for cryopreserving and isolating adipocyte nuclei is proposed. Adipose tissue samples were directly analyzed (NoCRYO0h), or stored at -196°C for 7 days without 10% dimethyl sulfoxide (DMSO) (CRYO-WO-DMSO) or with DMSO (CRYO-W-DMSO). To determine the effect of DMSO on cryopreservation treatment, adipose tissue samples were stored at 4°C for 24 h with 10% DMSO (NoCRYO-W-DMSO-24h) and without (NoCRYO-WO-DMSO-24h). Samples were processed in isolation buffer, and nuclear integrity was measured by flow cytometry. The coefficient of variation, forward scatter, side scatter, and number of nuclei analyzed were evaluated. Pea (Pisum sativum) was used to measure the amount of DNA. All groups contained similar amounts of DNA to previously reported values and a satisfactory number of nuclei were analyzed. CRYO-W-DMSO presented a higher coefficient of variation (3.19 ± 0.09) compared to NoCRYO-0h (1.85 ± 0.09) and CRYO-WO-DMSO (2.02 ± 0.02). The coefficient of variation was increased in NoCRYO-W-DMSO-24h (3.80 ± 0.01) compared to NoCRYO-WO-DMSO-24h (2.46 ± 0.03). These results relate DMSO presence to DNA damage independently of the cryopreservation process. CRYO-W-DMSO showed increased side scatter (93.46 ± 5.03) compared to ) and CRYO-WO-DMSO (48.01 ± 2.28), indicating that cryopreservation with DMSO caused chromatin condensation and/or nuclear fragmentation. CRYO-W-DMSO and CRYO-WO-DMSO presented lower forward scatter (186.33 ± 9.33 and 196.89 ± 26.86, respectively) compared to , indicating that cryopreservation reduced nuclei size. Thus, a simple method for cryopreservation and isolation of adipocyte nuclei causing less damage to DNA integrity was proposed.

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Controlled ice nucleation in cryopreservation – A review

Cited by 226 publications

References 75 publications

Direct observation of ice nucleation events on individual atmospheric particles

Direct observation of ice nucleation events on individual atmospheric particles

Points to Consider in the Development of Seed Stocks of Pluripotent Stem Cells for Clinical Applications: International Stem Cell Banking Initiative (ISCBI)

Protocol to cryopreserve and isolate nuclei from adipose tissue without dimethyl sulfoxide

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