Controlled enzymatic hydrolysis and synthesis of lignin cross-linked chitosan functional hydrogels

Beer, Bianca; Bartolome, Miguel Jimenez; Berndorfer, Lisa; Bochmann, Günther; Guebitz, Georg M.; Nyanhongo, Gibson S.

doi:10.1016/j.ijbiomac.2020.08.030

Cited by 19 publications

(7 citation statements)

References 43 publications

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“…101,102 Green alternatives, including enzymatic oxidation utilizing fungal lignin peroxidases or laccases have been reported. [103][104][105] Nevertheless, periodate oxidation promises excellent scalability and the electrochemical synthesis route for sodium periodate makes the oxidant an attractive green option. 106 The oxidative generation of quinones in raw, non-demethylated lignin commonly suffers from a low abundancy of catechols.…”

Section: Activation Of Ligninmentioning

confidence: 99%

Organic transformation of lignin into mussel-inspired glues: next-generation 2K adhesive for setting corals under saltwater

Choi,

Lossada,

Walter

et al. 2024

Green Chem.

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Section: Activation Of Ligninmentioning

confidence: 99%

Organic transformation of lignin into mussel-inspired glues: next-generation 2K adhesive for setting corals under saltwater

Choi,

Lossada,

Walter

et al. 2024

Green Chem.

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“…Similarly, various crosslinking molecules such as simple phenolic compounds, glutaraldehyde, epichlorohydrin, ethylene glycol, diglycidyl ether and sodium tripolyphosphate etc. are used [ 11 , 88 , 227 , 228 ]. Glutaraldehyde mediated cross-linking of chitosan by forming a Schiff base (imine) is the most studied technique which leads to condensation reaction between the aldehyde function and a primary amine group from the chitosan chain in the presence of labile hydrogen.…”

Section: Tailoring Chitosan For Specific Applicationsmentioning

confidence: 99%

Chitosan: Sources, Processing and Modification Techniques

Pellis

Guebitz

Nyanhongo

2022

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133

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Chitosan, a copolymer of glucosamine and N-acetyl glucosamine, is derived from chitin. Chitin is found in cell walls of crustaceans, fungi, insects and in some algae, microorganisms, and some invertebrate animals. Chitosan is emerging as a very important raw material for the synthesis of a wide range of products used for food, medical, pharmaceutical, health care, agriculture, industry, and environmental pollution protection. This review, in line with the focus of this special issue, provides the reader with (1) an overview on different sources of chitin, (2) advances in techniques used to extract chitin and converting it into chitosan, (3) the importance of the inherent characteristics of the chitosan from different sources that makes them suitable for specific applications and, finally, (4) briefly summarizes ways of tailoring chitosan for specific applications. The review also presents the influence of the degree of acetylation (DA) and degree of deacetylation (DDA), molecular weight (Mw) on the physicochemical and biological properties of chitosan, acid-base behavior, biodegradability, solubility, reactivity, among many other properties that determine processability and suitability for specific applications. This is intended to help guide researchers select the right chitosan raw material for their specific applications.

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“…We believe that the efficiency of CEC is due to the presence of enzymes that have hydrolytic action, especially for obtaining chitooligosaccharides [46,56,62,63].…”

Section: Effect Of Hydrolysis Time Using Cecmentioning

confidence: 99%

Crude Enzyme Concentrate of Filamentous Fungus Hydrolyzed Chitosan to Obtain Oligomers of Different Sizes

et al. 2023

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Chitosan is a non-cytotoxic polysaccharide that, upon hydrolysis, releases oligomers of different sizes that may have antioxidant, antimicrobial activity and the inhibition of cancer cell growth, among other applications. It is, therefore, a hydrolysis process with great biotechnological relevance. Thus, this study aims to use a crude enzyme concentrate (CEC) produced by a filamentous fungus to obtain oligomers with different molecular weights. The microorganism was cultivated in a liquid medium (modified Czapeck—with carboxymethylcellulose as enzyme inducer). The enzymes present in the CEC were identified by LC-MS/MS, with an emphasis on cellobiohydrolase (E.C 3.2.1.91). The fungus of the Aspergillus genus was identified by amplifying the ITS1-5.8S-ITS2 rDNA region and metaproteomic analysis, where the excreted enzymes were identified with sequence coverage greater than 84% to A. nidulans. Chitosan hydrolysis assays compared the CEC with the commercial enzyme (Celluclast 1.5 L®). The ability to reduce the initial molecular mass of chitosan by 47.80, 75.24, and 93.26% after 2.0, 5.0, and 24 h of reaction, respectively, was observed. FTIR analyses revealed lower absorbance of chitosan oligomers’ spectral signals, and their crystallinity was reduced after 3 h of hydrolysis. Based on these results, we can conclude that the crude enzyme concentrate showed a significant technological potential for obtaining chitosan oligomers of different sizes.

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Controlled enzymatic hydrolysis and synthesis of lignin cross-linked chitosan functional hydrogels

Cited by 19 publications

References 43 publications

Organic transformation of lignin into mussel-inspired glues: next-generation 2K adhesive for setting corals under saltwater

Organic transformation of lignin into mussel-inspired glues: next-generation 2K adhesive for setting corals under saltwater

Chitosan: Sources, Processing and Modification Techniques

Crude Enzyme Concentrate of Filamentous Fungus Hydrolyzed Chitosan to Obtain Oligomers of Different Sizes

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