Controlled electrophoretic deposition of multifunctional nanomodules for bioelectrochemical applications

Dondapati, Srujan Kumar; Lozano-Sánchez, Pablo; Katakis, Ioanis

doi:10.1016/j.bios.2008.03.022

Cited by 9 publications

(10 citation statements)

References 25 publications

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“…In most of the cases where there is a preconcentration step with immunoparticles, there is immobilization of the particle-bacteria conjugate on the surface. Regardless the modifications done to it, the core nature of the particle used to bind to the pathogen most frequently dictates the immobilization procedure; gold or other metal nanoparticles can be immobilized by the use of glutaraldehyde [11,22] or by electrophoretic depositions [23]; alternatively the use of paramagnetic particles tend to be attached by the means of magnetic forces [11].…”

Section: Introductionmentioning

confidence: 99%

Screen-printed integrated microsystem for the electrochemical detection of pathogens

Mata

Bejarano

Botero

et al. 2010

Electrochimica Acta

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Section: Introductionmentioning

confidence: 99%

Screen-printed integrated microsystem for the electrochemical detection of pathogens

Mata

Bejarano

Botero

et al. 2010

Electrochimica Acta

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“…Metal nanoparticles have received great attention in recent years in different fields due to their enormous potential. It is very promising for practical applications in many diverse fields, such as electronic nanodevices [20], molecular catalysts [21], multifunctional reagent and biosensors [22,23]. Chemically modified carbon paste electrodes (CM-CPEs) have been widely investigated because of the advantages of using a CPE include convenient preparation by mixing a modifier with the paste, the availability of a wide potential range for analysis, easily renewable surface, and simplicity of fabrication.…”

Section: Introductionmentioning

confidence: 99%

Voltammetric Determination of Trace Antiepileptic Gabapentin with a Silver‐Nanoparticle Modified Multiwalled Carbon Nanotube Paste Electrode

2011

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For the first time, a novel carbon nanotube bed electrode impregnated with silver-nanoparticles (AgNPs) for the determination of trace amounts of gabapentin (GBP) is described. We synthesized the AgNPs via a new procedure. The voltammetric behavior of the electrode was investigated by cyclic voltammetry. There were linear relationships in the ranges from 3.1 10 À9 to 2.9 10 À2 M and from 1.0 10 À8 to 1.0 10 À2 GBP with square wave and differential pulse voltammetric peak currents, respectively. The detection limits were 5.6 10 À10 and 9.7 10 À9 M, respectively. The electrode showed excellent response over a period of 2 months and was successfully applied in human plasma and pharmaceutical capsular products.

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“…A third approach consists of modifying colloidal particles with biological or chemical functionalities. , The electrophoretic deposition of these bio/nanoparticles has been used previously to produce enzyme biosensors, e.g. by depositing nanomodules comprised of Au nps modified with a redox polymer and glucose oxidase . DNA sensors were also produced using thiol-modified oligonucleotide probes conjugated to colloidal gold nanoparticles .…”

Section: Introductionmentioning

confidence: 99%

Electrodeposition of Polymer Nanodots with Controlled Density and Their Reversible Functionalization by Polyhistidine-Tag Proteins

et al. 2012

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We present a simple and rapid procedure for producing polymer-coated substrates that can be easily functionalized by ion-chelating proteins. The procedure consists of depositing 18 nm metal-chelating cyclam-modified polymer nanoparticles (cyclam-nps) onto a conductive substrate (an Indium Tin Oxide (ITO) electrode) from an aqueous dispersion of Cu(2+)-loaded cyclam-nps while being subjected to a direct current (DC) field. The density of deposited nps as measured by AFM is shown to be in direct correlation to the concentration of nps in the dispersion with deposition of the particles taking less than 5 s. Because of the functionalization of the nps with cyclam groups, they can be used as anchoring sites for 6-Histidine (6-His) tagged proteins through complexation with divalent metal ions. In this work 6-His Green Fluorescent Protein (6-His GFP) is used as a model protein. The characterization by fluorescence microscopy clearly shows that the protein affinity was ion dependent and that the 6-His GFP density can be controlled by np density, which is itself easily tunable. AFM observations confirmed the immobilization of 6-His GFP onto cyclam-nps and its subsequent removal by treatment with ethylenediaminetetraacetic acid (EDTA).

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Controlled electrophoretic deposition of multifunctional nanomodules for bioelectrochemical applications

Cited by 9 publications

References 25 publications

Screen-printed integrated microsystem for the electrochemical detection of pathogens

Screen-printed integrated microsystem for the electrochemical detection of pathogens

Voltammetric Determination of Trace Antiepileptic Gabapentin with a Silver‐Nanoparticle Modified Multiwalled Carbon Nanotube Paste Electrode

Electrodeposition of Polymer Nanodots with Controlled Density and Their Reversible Functionalization by Polyhistidine-Tag Proteins

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