Controlled drug release for tissue engineering

Rambhia, Kunal J.; Peter, X.

doi:10.1016/j.jconrel.2015.08.049

Cited by 183 publications

(118 citation statements)

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“…The release of proteins from electrospun 3D matrices has been studied for a number of polymers, and the released proteins are shown to demonstrate their intrinsic activity. [12,13] The loading of fiber surface with protein-specific receptors of cell adhesion using the surface activation by chemical modification, [14][15][16] argon plasma, [17] cold plasma, [18][19][20] and laser [15] or UV illumination [21,22] has been shown to increase cell adhesion. However, exposure of proteins on the surface of the fibers electrospun from the blends of proteins and polymers as well as their lifespan on the surface of fibers is poorly studied.…”

Section: Introductionmentioning

confidence: 99%

Human serum albumin in electrospun PCL fibers: structure, release, and exposure on fiber surface

Chernonosova

Kvon

Stepanova

et al. 2016

Polym. Adv. Technol.

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Human serum albumin (HSA) introduced to the fibers produced by electrospinning from HSA and polycaprolactone (PCL) solutions in hexafluoroisopropanol has been studied in terms of its structure, release from the fibers, stability of interaction with basic polymer, accessibility for protease attack, and cellular receptors, as well as dependence of the studied parameters on the protein concentration in fibers. A limited part of the protein leaves the fibers right after soaking with water, whereas the remaining protein stays tightly bound to fibers for a long time because protein nanoparticles are tightly integrated with PCL, as shown by small‐angle X‐ray scattering. As has been demonstrated, the proteins leave the fibers in complexes with PCL. X‐ray photoelectron spectroscopy demonstrates that the protein concentration on the fiber surface is higher than the concentration in electrospinning solution. The surface‐exposed protein is recognized by cell receptors and is partially hydrolyzed by proteinase K. The data on pulse protein release, presence of PCL in the protein released from matrixes, overrepresentation of the protein on the fiber surface, and tight interaction of protein with PCL may be useful for rational design of electrospun scaffolds intended for drug delivery and tissue engineering. Copyright © 2016 John Wiley & Sons, Ltd.

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Section: Introductionmentioning

confidence: 99%

Human serum albumin in electrospun PCL fibers: structure, release, and exposure on fiber surface

Chernonosova

Kvon

Stepanova

et al. 2016

Polym. Adv. Technol.

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“…[1] More recently, this technology has also been adapted to the generation of in vitro tissue models for applications in improved drug screening and personalized medicine. [2] In an engineered tissue construct, the cells that form the biological basis, the growth/differentiation factors that induce proper cellular functions, and the biomaterial scaffold that mimics the extracellular matrix (ECM), are the basic elements typically necessary to achieve optimal tissue biofabrication. [3, 4] In particular, the scaffold as a critical component in most scenarios, provide structural support for cell attachment, proliferation, and differentiation.…”

mentioning

confidence: 99%

Aqueous Two‐Phase Emulsion Bioink‐Enabled 3D Bioprinting of Porous Hydrogels

et al. 2018

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The three-dimensional (3D) bioprinting technology provides programmable and customizable platforms to engineer cell-laden constructs mimicing human tissues for a wide range of biomedical applications. However, the encapsulated cells are often restricted in spreading and proliferation by dense biomaterial networks from gelation of bioinks. Herein, we report a novel cell-benign approach to directly bioprint porous-structured hydrogel constructs by using an aqueous two-phase emulsion bioink. The bioink, which contains two immiscible aqueous phases of cell/gelatin methacryloyl (GelMA) mixture and poly(ethylene oxide) (PEO), is photocrosslinked to fabricate predesigned cell-laden hydrogel constructs by extrusion bioprinting or digital micromirror device-based stereolithographic bioprinting. Porous structure of the 3D-bioprinted hydrogel construct is formed by subsequently removing the PEO phase from the photocrosslinked GelMA hydrogel. Three different cells (human hepatocellular carcinoma cells, human umbilical endothelial cells, and NIH/3T3 mouse embryonic fibroblasts) within the 3D-bioprinted porous cell-laden hydrogel patterns showed enhanced cell viability, spreading, and proliferation compared to the standard (i.e. non-porous) hydrogel constructs. The new 3D bioprinting strategy is believed to provide a robust and versatile platform to engineer porous-structured tissue constructs and their models for a variety of applications in tissue engineering, regenerative medicine, and personalized therapeutics.

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“…The results obtain from this study shows that PCL nanofibers containing chitosan nanoparticle has a porous structure which help the sustained release of G‐CSF. Protein‐loaded nanofiber scaffolds could be fabricated by several techniques such as, physical absorption, covalent immobilization, emulsion electrospinning, coaxial electrospinning, and suspension electrospinning . Vakilian et al studied the control release of BSA and TGF‐β1 from PCL nanofibers containing protein‐loaded chitosan nanoparticles.…”

Section: Discussionmentioning

confidence: 99%

“…Proteinloaded nanofiber scaffolds could be fabricated by several techniques such as, physical absorption, covalent immobilization, emulsion electrospinning, coaxial electrospinning, and suspension electrospinning. [22][23][24][25][26] Vakilian et al studied the control release of BSA and TGF-b1 from PCL nanofibers containing protein-loaded chitosan nanoparticles. They have reported the sustained release of TGF-b1 and its effect on stem cell differentiation.…”

Section: Discussionmentioning

confidence: 99%

G‐CSF loaded nanofiber/nanoparticle composite coated with collagen promotes wound healing in vivo

Tanha

Rafiee-Tehrani

Abdollahi

et al. 2017

J Biomedical Materials Res

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Sustained release of functional growth factors can be considered as a beneficial methodology for wound healing. In this study, recombinant human granulocyte colony-stimulating factor (G-CSF)-loaded chitosan nanoparticles were incorporated in Poly(ε-caprolactone) (PCL) nanofibers, followed by surface coating with collagen type I. Physical and mechanical properties of the PCL nanofibers containing G-CSF loaded chitosan nanoparticles PCL/NP(G-CSF) and in vivo performance for wound healing were investigated. G-CSF structural stability was evaluated through SDS_PAGE, reversed phase (RP) HPLC and size-exclusion chromatography, as well as circular dichroism. Nanofiber/nanoparticle composite scaffold was demonstrated to have appropriate mechanical properties as a wound dresser and a sustained release of functional G-CSF. The PCL/NP(G-CSF) scaffold showed a suitable proliferation and well-adherent morphology of stem cells. In vivo study and histopathological evaluation outcome revealed that skin regeneration was dramatically accelerated under PCL/NP(G-CSF) as compared with control groups. Superior fibroblast maturation, enhanced collagen deposition and minimum inflammatory cells were also the beneficial properties of PCL/NP(G-CSF) over the commercial dressing. The synergistic effect of extracellular matrix-mimicking nanofibrous membrane and G-CSF could develop a suitable supportive substrate in order to extensive utilization for the healing of skin wounds. © 2017 Wiley Periodicals Inc. J Biomed Mater Res Part A: 105A: 2830-2842, 2017.

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Controlled drug release for tissue engineering

Cited by 183 publications

References 118 publications

Human serum albumin in electrospun PCL fibers: structure, release, and exposure on fiber surface

Human serum albumin in electrospun PCL fibers: structure, release, and exposure on fiber surface

Aqueous Two‐Phase Emulsion Bioink‐Enabled 3D Bioprinting of Porous Hydrogels

G‐CSF loaded nanofiber/nanoparticle composite coated with collagen promotes wound healing in vivo

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