Controlled Delivery of Fibroblast Growth Factor-9 from Biodegradable Poly(ester amide) Fibers for Building Functional Neovasculature

Said, Somiraa S.; Pickering, J. Geoffrey; Mequanint, Kibret

doi:10.1007/s11095-014-1423-2

Cited by 23 publications

(20 citation statements)

References 39 publications

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“…Other proteins, such as fibroblast growth factor 9 (FGF9) and vaccination antigens, have also been proven to retain their biological activity in PEA-based carriers (dos Santos et al, 2009;Said et al, 2014). As further proof of CXCL1 activity after release, we observed significant in vivo evidence of microsphere released CXCL1.…”

Section: Discussionsupporting

confidence: 55%

CXCL1 microspheres: a novel tool to stimulate arteriogenesis

Caolo

Vries

Zupancich³

et al. 2015

Drug Delivery

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Context: After arterial occlusion, diametrical growth of pre-existing natural bypasses around the obstruction, i.e. arteriogenesis, is the body's main coping mechanism. We have shown before that continuous infusion of chemokine (C-X-C motif) ligand 1 (CXCL1) promotes arteriogenesis in a rodent hind limb ischemia model. Objective: For clinical translation of these positive results, we developed a new administration strategy of local and sustained delivery. Here, we investigate the therapeutic potential of CXCL1 in a drug delivery system based on microspheres. Materials and methods: We generated poly(ester amide) (PEA) microspheres loaded with CXCL1 and evaluated them in vitro for cellular toxicity and chemokine release characteristics. In vivo, murine femoral arteries were ligated and CXCL1 was administered either intra-arterially via osmopump or intramuscularly encapsulated in biodegradable microspheres. Perfusion recovery was measured with Laser-Doppler. Results: The developed microspheres were not cytotoxic and displayed a sustained chemokine release up to 28 d in vitro. The amount of released CXCL1 was 100-fold higher than levels in native ligated hind limb. Also, the CXCL1-loaded microspheres significantly enhanced perfusion recovery at day 7 after ligation compared with both saline and non-loaded conditions (55.4 ± 5.0% CXCL1-loaded microspheres versus 43.1 ± 4.5% non-loaded microspheres; n ¼ 8-9; p50.05). On day 21 after ligation, the CXCL1-loaded microspheres performed even better than continuous CXCL1 administration (102.1 ± 4.4% CXCL1-loaded microspheres versus 85.7 ± 4.8% CXCL1 osmopump; n ¼ 9; p50.05). Conclusion: Our results demonstrate a proof of concept that sustained, local delivery of CXCL1 encapsulated in PEA microspheres provides a new tool to stimulate arteriogenesis in vivo.

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Section: Discussionsupporting

confidence: 55%

CXCL1 microspheres: a novel tool to stimulate arteriogenesis

Caolo

Vries

Zupancich³

et al. 2015

Drug Delivery

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“…Cells on FGF9-supplemented PEA mats showed upregulation of PDGFRβ dependent on the concentration and cell type. 31 With regard to morpholino-2,5-dione polymerization (see section 2), lipasecatalyzed ROP of isoleucine-based 3(S)-sec-butylmorpholine-2,5-dione (BMD) was described to afford poly(BMD), an optically active PEA with specific stereoregularity (dyads) ( Fig. 1B).…”

Section: General Aspects Of Poly(ester Amide) Synthesismentioning

confidence: 99%

Poly(ester amide)s: recent insights into synthesis, stability and biomedical applications

2016

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“…[11] Using blend and emulsion electrospinning approaches, a differential and sustained release of growth factors from PEA fibres has been achieved. [115,119] Data from ischemic mice limb studies had shown a considerable functional recovery when the growth factors were delivered from a fibrous mat. [118] Non-covalent incorporation of growth factors is another loading strategy that has been used for the regeneration of tissues.…”

Section: Growth Factor Loading Strategies and Release Kineticsmentioning

confidence: 99%

Tissue engineering and regenerative therapeutics: The nexus of chemical engineering and translational medicine

et al. 2021

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Since its emergence nearly 30 years ago with the goal of fabricating replacement human tissues or assisting the body in repairing itself, several advances have been made in the fields of tissue engineering and regenerative medicine. The assembly of living cells into tissues is akin to the scale-up operation of a chemical or biochemical process with several modules or units affecting the final product. The major modules or units in tissue engineering and regenerative medicine are cells, biodegradable scaffolds, bioreactors, and biomolecules. Each module is analogous to unit operations, where individual units must come together to successfully design engineered tissues for therapeutic and drug discovery applications. Basic principles of biological sciences, chemical engineering, materials chemistry, and polymer processing are frequently employed. More specifically, knowledge about viscous fluid flow, mass transfer, and reaction engineering is essential in tissue engineering. In this review article, we present the nexus between chemical engineering and tissue engineering for the rational design of engineered tissues or injectable cellladen hydrogel systems.

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Controlled Delivery of Fibroblast Growth Factor-9 from Biodegradable Poly(ester amide) Fibers for Building Functional Neovasculature

Abstract: This study supports the premise of controlled delivery of FGF9 from PEA electrospun fibers for potential therapeutic angiogenesis applications.

Cited by 23 publications

References 39 publications

CXCL1 microspheres: a novel tool to stimulate arteriogenesis

CXCL1 microspheres: a novel tool to stimulate arteriogenesis

Poly(ester amide)s: recent insights into synthesis, stability and biomedical applications

Tissue engineering and regenerative therapeutics: The nexus of chemical engineering and translational medicine

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