Controlled comparison of Interceed and amniotic membrane graft in the prevention of postoperative adhesions in the rabbit uterine horn model

Arora, Mala; Jaroudi, Kamal; Hamilton, C.J.C.M.; Dayel, Fouad Al

doi:10.1016/0028-2243(94)90035-3

Cited by 37 publications

(20 citation statements)

References 9 publications

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“…Evaluation of the percentage of graft surface involved by adhesions is simple and accurate; however, it is only a single property. Our results differ from those of other studies, which may be due to the use of fresh, not stored, amniotic membrane in our study, while other investigators used amnion which was stored for 4-10 weeks [21]. Our results are impressive and statistically significant.…”

Section: B Acontrasting

confidence: 57%

Evaluation of Seprafilm and Amniotic Membrane as Adhesion Prophylaxis in Mesh Repair of Abdominal Wall Hernia in Rats

et al. 2000

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Introduction: Adhesion formation following abdominal wall hernia repair with prosthetic mesh may lead to intestinal obstruction and enterocutaneous fistula. Physical barriers, namely, human amniotic membrane (HAM) or Seprafilm (Genzyme, Cambridge, Mass., USA), a bio-absorbable, translucent membrane composed of carboxymethylcellulose and hyaluronic acid, have been reported to prevent postsurgical intra-abdominal adhesions. Objective: Evaluating the effect of HAM and Seprafilm in preventing adhesion formation in the rat model of ventral hernia repair with polypropylene mesh (PPM). Material and Methods: Sixty female Sprague-Dawley rats were divided into three groups. A full-thickness abdominal wall defect was created in each animal. Control animals had the PPM sutured into the defect, whereas in the other two groups, either HAM or Seprafilm were laid over the abdominal viscera before the repair with PPM. Half of the animals in each group were sacrificed on the 21st postoperative day. The remaining rats of the same group were re-operated on the 42nd day for investigation and measurement of the adhesion area in relation to the graft area. Results: Direct mesh repair showed 52.8 and 56% area adhesion formation 3 and 6 weeks postoperatively, respectively. The HAM barrier covered with mesh repair demonstrated 0 and 0.96% area adhesion formation, and the Seprafilm-covered mesh repair showed 0 and 0% area adhesion formation 3 and 6 weeks postoperatively, respectively. Uncovered mesh showed a significantly larger adhesion area than both covered mesh (p = 0.001 and 0.001). Both HAM and Seprafilm were equally effective in preventing postoperative adhesions. Conclusions: HAM and Seprafilm proved to be an effective antiadhesive barrier in PPM repair of abdominal wall hernia.

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Section: B Acontrasting

confidence: 57%

Evaluation of Seprafilm and Amniotic Membrane as Adhesion Prophylaxis in Mesh Repair of Abdominal Wall Hernia in Rats

et al. 2000

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“…Nowadays, in addition to the use in ophthalmology, amnion is widely applied in surgery and wound treatment e.g. for burned skin, bedsore, ulcers (Faulk et al 1980;Subrahmanyam 1995;Gruss and Jirsch 1978;Ward et al 1989;Gajiwala and Gajiwala 2004), reconstruction of artificial vagina (Burger 1937;Dhall 1984;Nisolle and Donnez 1992), in head and neck surgery (Zohar et al 1987) as well as to prevent tissue adhesion in surgical procedures of the abdomen, head and pelvis (Rennekampff et al 1994;Arora et al 1994;Young et al 1991).…”

Section: Introductionmentioning

confidence: 97%

The influence of various storage conditions on cell viability in amniotic membrane

Hennerbichler

Reichl

Pleiner

et al. 2006

Cell Tissue Banking

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Up to now freeze-dried, gamma-sterilised or glycerol-preserved amniotic membranes (AMs) have widely been used in the field of ophthalmology and wound care (e.g. leg ulcers, burns). After some preservation processes in use, like freeze-drying or glycerol-preserving, the cells in the AM are no longer viable. Within this study we evaluated the influence of different short-term and long-term storage conditions on cell viability in AM. Therefore AMs from cesarean section placentae were washed and biopsied to evaluate the microbiological status and to determine the viability of the tissue. Additionally, viability under various storage conditions was examined by assessment of mitochondrial activity. Preservation included temperatures above and below 0 degrees C as well as various media compositions. As expected, cell viability in amnion decreases during storage, in fact the effect was more pronounced when stored frozen, but the higher viability of amnion obtained by storage above 0 degrees C with medium is associated with the limitation to a short period of storage of about 28 days. The evaluated preservation methods are the basis for future non-clinical in-vivo studies in which the possible benefit of amnion as a viable biomaterial in wound healing will be investigated.

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“…In 1910, Davis [1] was the first to use AM in skin transplantation. Subsequently, it has been widely used in management of burns [2,3]; as a surgical dressing [4,5]; surgical reconstruction of the oral cavity [6], bladder [7], and vagina [8,9]; occlusion of pericardium [10]; and in the prevention of surgical adhesions [11,12]. Although, as discussed below the inherent biological qualities in this membrane substrate have prompted its widespread and successful use, there are some limitations.…”

Section: Introductionmentioning

confidence: 99%

Preservation, sterilization and de-epithelialization of human amniotic membrane for use in ocular surface reconstruction

et al. 2010

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Controlled comparison of Interceed and amniotic membrane graft in the prevention of postoperative adhesions in the rabbit uterine horn model

Cited by 37 publications

References 9 publications

Evaluation of Seprafilm and Amniotic Membrane as Adhesion Prophylaxis in Mesh Repair of Abdominal Wall Hernia in Rats

Evaluation of Seprafilm and Amniotic Membrane as Adhesion Prophylaxis in Mesh Repair of Abdominal Wall Hernia in Rats

The influence of various storage conditions on cell viability in amniotic membrane

Preservation, sterilization and de-epithelialization of human amniotic membrane for use in ocular surface reconstruction

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