Controlled Angiogenesis in Peptide Nanofiber Composite Hydrogels

Wickremasinghe, Navindee C.; Kumar, Vivek; Shi, Siyu; Hartgerink, Jeffrey D.

doi:10.1021/acsbiomaterials.5b00210

Cited by 35 publications

(29 citation statements)

References 66 publications

(253 reference statements)

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“…Interestingly, we have recently demonstrated that the propensity for hydrogels to promote a robust angiogenic response can also be modulated by the release of a variety of growth factors in a temporally controlled manner. In this related study, Wickremasinghe et al showed the development of mature vasculature in similar hydrogel preparations by the temporally controlled release of PlGF-1 in liposomes [20,29]. Similar to this study, and previously published work, the ability for MDP to rapidly infiltrate cells creates a niche for the rapid (within 7 days) development of mature SMC lined microvessels.…”

Section: Discussionsupporting

confidence: 85%

Treatment of hind limb ischemia using angiogenic peptide nanofibers

Liu²,

et al. 2016

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For a proangiogenic therapy to be successful, it must promote the development of mature vasculature for rapid reperfusion of ischemic tissue. Whole growth factor, stem cell, and gene therapies have yet to achieve the clinical success needed to become FDA-approved revascularization therapies. Herein, we characterize a biodegradable peptide-based scaffold engineered to mimic VEGF and self-assemble into a nanofibrous, thixotropic hydrogel, SLanc. We found that this injectable hydrogel was rapidly infiltrated by host cells and could be degraded while promoting the generation of neovessels. In mice with induced hind limb ischemia, this synthetic peptide scaffold promoted angiogenesis and ischemic tissue recovery, as shown by Doppler-quantified limb perfusion and a treadmill endurance test. Thirteen-month-old mice showed significant recovery within 7 days of treatment. Biodistribution studies in healthy mice showed that the hydrogel is safe when administered intramuscularly, subcutaneously, or intravenously. These preclinical studies help establish the efficacy of this treatment for peripheral artery disease due to diminished microvascular perfusion, a necessary step before clinical translation. This peptide-based approach eliminates the need for cell transplantation or viral gene transfection (therapies currently being assessed in clinical trials) and could be a more effective regenerative medicine approach to microvascular tissue engineering.

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Section: Discussionsupporting

confidence: 85%

Treatment of hind limb ischemia using angiogenic peptide nanofibers

Liu²,

et al. 2016

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“…A number of strategies have been investigated for the presentation of VEGF signaling in scaffolds; self-assembling peptide hydrogels presenting high density of epitopes mimicking VEGF and other growth factors have been developed [37,38,39,40,41,42,43,44,45,46,47,48,49,50,51,52,53,54,55,56,57,58,59,60,61]. Using mimics of VEGF, large macromolecules can be functionalized.…”

Section: Strategies For Therapeutic Vascularizationmentioning

confidence: 99%

Challenges in Translating from Bench to Bed-Side: Pro-Angiogenic Peptides for Ischemia Treatment

Petrak¹,

Vissapragada

Shi

et al. 2019

Molecules

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We describe progress and obstacles in the development of novel peptide-hydrogel therapeutics for unmet medical needs in ischemia treatment, focusing on the development and translation of therapies specifically in peripheral artery disease (PAD). Ischemia is a potentially life-threatening complication in PAD, which affects a significant percentage of the elderly population. While studies on inducing angiogenesis to treat PAD were started two decades ago, early results from animal models as well as clinical trials have not yet been translated into clinical practice. We examine some of the challenges encountered during such translation. We further note the need for sustained angiogenic effect involving whole growth factor, gene therapy and synthetic growth factor strategies. Finally, we discuss the need for tissue depots for de novo formation of microvasculature. These scaffolds can act as templates for neovasculature development to improve circulation and healing at the preferred anatomical location.

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“…This can be achieved through the entrapment of the therapeutic factors within the hydrogel, 62,63 or the covalent immobilization of the factors to the hydrogel matrix; 64 for the latter strategy, the therapeutic payload would be released upon cellular degradation of the factor-matrix linkages. Alternatively, hydrogels can serve as the structural scaffold for angiogenesis, in which the three-dimensional matrix and the angiogenic functional motifs decorating the peptide nanofibers synergize to provide superior conditions for angiogenesis.…”

Section: Angiogenesismentioning

confidence: 99%

Harnessing supramolecular peptide nanotechnology in biomedical applications

Chan

Lee

Zhuo

et al. 2017

IJN

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The harnessing of peptides in biomedical applications is a recent hot topic. This arises mainly from the general biocompatibility of peptides, as well as from the ease of tunability of peptide structure to engineer desired properties. The ease of progression from laboratory testing to clinical trials is evident from the plethora of examples available. In this review, we compare and contrast how three distinct self-assembled peptide nanostructures possess different functions. We have 1) nanofibrils in biomaterials that can interact with cells, 2) nanoparticles that can traverse the bloodstream to deliver its payload and also be bioimaged, and 3) nanotubes that can serve as cross-membrane conduits and as a template for nanowire formation. Through this review, we aim to illustrate how various peptides, in their various self-assembled nanostructures, possess great promise in a wide range of biomedical applications and what more can be expected.

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Controlled Angiogenesis in Peptide Nanofiber Composite Hydrogels

Cited by 35 publications

References 66 publications

Treatment of hind limb ischemia using angiogenic peptide nanofibers

Treatment of hind limb ischemia using angiogenic peptide nanofibers

Challenges in Translating from Bench to Bed-Side: Pro-Angiogenic Peptides for Ischemia Treatment

Harnessing supramolecular peptide nanotechnology in biomedical applications

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