Controlled and Localized Nitric Oxide Precursor Delivery From Chitosan Gels to Staphylococcus aureus Biofilms

Hasan, Sayeed; Thomas, Nicky; Thierry, Benjamin; Prestidge, Clive A.

doi:10.1016/j.xphs.2017.08.006

Cited by 12 publications

(9 citation statements)

References 39 publications

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“…The application of topical compounds, e.g., in treating psoriasis (Kusuba et al 2017) or other inflammatory diseases, and its effect on susceptibility to infections can be studied in depth with skin models, especially skin explants since the skin surface closely resembles the in vivo situation. In addition, novel anti-infective approaches, such as nano-sponges that absorb secreted bacterial toxins (Hu et al 2013;Escajadillo et al 2017) or other innovative antimicrobial delivery systems (Hasan et al 2017), can be tested for their efficacy with the skin model. Similarly, our group recently showed the protective effect of antibodies against S. aureus toxins within the context of the human skin explant model (Olaniyi et al 2018).…”

Section: Use Of Human Skin Models In Anti-infective Researchmentioning

confidence: 99%

Human Organotypic Models for Anti-infective Research

Hendriks¹,

Cruz²,

Soldaini³

et al. 2018

Current Topics in Microbiology and Immunology

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The use of human organotypic models for biomedical research is experiencing a significant increase due to their biological relevance, the possibility to perform high-throughput analyses, and their cost efficiency. In the field of anti-infective research, comprising the search for novel antipathogenic treatments including vaccines, efforts have been made to reduce the use of animal models. That is due to two main reasons: unreliability of data obtained with animal models and the increasing willingness to reduce the use of animals in research for ethical reasons. Human three-dimensional (3-D) models may substitute and/or complement in vivo studies, to increase the translational value of preclinical data. Here, we provide an overview of recent studies utilizing human organotypic models, resembling features of the cervix, intestine, lungs, brain, and skin in the context of anti-infective research. Furthermore, we focus on the future applications of human skin models and present methodological protocols to culture human skin equivalents and human skin explants.

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Section: Use Of Human Skin Models In Anti-infective Researchmentioning

confidence: 99%

Human Organotypic Models for Anti-infective Research

Hendriks¹,

Cruz²,

Soldaini³

et al. 2018

Current Topics in Microbiology and Immunology

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“…In addition, the concentration range in which the NO-releasing hydrogel demonstrated an antibacterial effect was not found to be toxic to the Vero mammalian cell. Similarly, GSNO-containing hydrogel films also demonstrated sustained NO release and significantly enhanced antibacterial activity against MRSA compared to the hydrogels without NO donors. , The sustained release of NO from CS hydrogels containing NO donors promoted the antibacterial activity along with excellent antibiofilm performance . Compared with aqueous RS-NO solutions, hydrogels significantly reduce the rate of NO release from the RSNOs donors.…”

Section: Stimuli-induced Antibacterial Activitymentioning

confidence: 85%

“…189,190 The sustained release of NO from CS hydrogels containing NO donors promoted the antibacterial activity along with excellent antibiofilm performance. 191 Compared with aqueous RS-NO solutions, hydrogels significantly reduce the rate of NO release from the RSNOs donors. This effect is attributed to a cage effect imposed by the hydrogel matrix, which favors the RS-NO radical pair recombination after the homolytic S− N cleavage.…”

Section: Photodynamic Therapy (Pdt)mentioning

confidence: 99%

Natural Polymer-Based Antimicrobial Hydrogels without Synthetic Antibiotics as Wound Dressings

et al. 2020

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Wound healing is usually accompanied by bacterial infection. The excessive use of synthetic antibiotics leads to drug resistance, posing a significant threat to human health. Hydrogel-based wound dressings aimed at mitigating bacterial infections have emerged as an effective wound treatment. The review presented herein particularly focuses on the hydrogels originating from natural polymers. To further enhance the performance of wound dressings, various strategies and approaches have been developed to endow the hydrogels with excellent broad-spectrum antibacterial activity. Those that are summarized in the current review are the hydrogels with intrinsic or stimuli-triggered bactericidal properties and others that serve as vehicles for loading antibacterial agents without synthetic antibiotics. Specific attention is paid to antimicrobial mechanisms and the antibacterial performance of hydrogels. Practical antibacterial applications to accelerate the wound healing employing these antibiotic-free hydrogels are also introduced along with the discussion on the current challenges and perspectives leading to new technologies.

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“…Chitosan gels with encapsulated NO donors, such as isosorbide mononitrate (ISMN) or GNSO, with or without further encapsulation of antibiotics, also showed sustained NO release over 72 h and was highly effective in reducing the viability and preventing biofilm formation of P. aeruginosa and S. aureus in vitro and in vivo [13,116,118]. NO release from these chitosan gels is influenced by their hydration state and more NO is released in response to higher amounts of wound exudates at the site of infection [13].…”

Section: Chitosan Gelsmentioning

confidence: 99%

Recent Developments in Nitric Oxide Donors and Delivery for Antimicrobial and Anti-Biofilm Applications

Poh

Rice

2022

Molecules

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The use of nitric oxide (NO) is emerging as a promising, novel approach for the treatment of antibiotic resistant bacteria and biofilm infections. Depending on the concentration, NO can induce biofilm dispersal, increase bacteria susceptibility to antibiotic treatment, and induce cell damage or cell death via the formation of reactive oxygen or reactive nitrogen species. The use of NO is, however, limited by its reactivity, which can affect NO delivery to its target site and result in off-target effects. To overcome these issues, and enable spatial or temporal control over NO release, various strategies for the design of NO-releasing materials, including the incorporation of photo-activable, charge-switchable, or bacteria-targeting groups, have been developed. Other strategies have focused on increased NO storage and delivery by encapsulation or conjugation of NO donors within a single polymeric framework. This review compiles recent developments in NO drugs and NO-releasing materials designed for applications in antimicrobial or anti-biofilm treatment and discusses limitations and variability in biological responses in response to the use of NO for bacterial eradiation.

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Controlled and Localized Nitric Oxide Precursor Delivery From Chitosan Gels to Staphylococcus aureus Biofilms

Cited by 12 publications

References 39 publications

Human Organotypic Models for Anti-infective Research

Human Organotypic Models for Anti-infective Research

Natural Polymer-Based Antimicrobial Hydrogels without Synthetic Antibiotics as Wound Dressings

Recent Developments in Nitric Oxide Donors and Delivery for Antimicrobial and Anti-Biofilm Applications

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