Controllable Self‐Assembly of RNA Tetrahedrons with Precise Shape and Size for Cancer Targeting

Li, Hui; Zhang, Kaiming; Pi, Fengmei; Guo, Sijin; Shlyakhtenko, Luda S.; Chiu, Wah; Shu, Dan; Guo, Peixuan

doi:10.1002/adma.201601976

Cited by 81 publications

(85 citation statements)

References 45 publications

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“…The difference of −12 kcal/mol prevents (2 + 3) duplex formation in the biosensor setup ((1 + 2) + 3); however, the presence of target strand makes the formation of (2 + 3) more favorable, by −85 kcal/mol, due to (1 + 4) association. The melting temperatures shown in Figure 3B for all duplexes were measured 43 to be 79.5 (77.8) °C for (1 + 2) duplex, 78.5 (76.3) °C for (2 + 3) duplex, and 78 (78) °C for (1 + 4) duplex and are in agreement with the predicted values (shown in parentheses).…”

Section: Resultssupporting

confidence: 85%

Fluorescence Blinking as an Output Signal for Biosensing

et al. 2016

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We demonstrate the first biosensing strategy that relies on quantum dot (QD) fluorescence blinking to report the presence of a target molecule. Unlike other biosensors that utilize QDs, our method does not require the analyte to induce any fluorescence intensity or color changes, making it readily applicable to a wide range of target species. Instead, our approach relies on the understanding that blinking, a single particle phenomenon, is obscured when several QDs lie within the detection volume of a confocal microscope. If QDs are engineered to aggregate when they encounter a particular target molecule, the observation of quasi-continuous emission should indicate its presence. As proof of concept, we programmed DNAs to drive rapid isothermal assembly of QDs in the presence of a target strand (oncogene K-ras). The assemblies, confirmed by various gel techniques, contained multiple QDs and were readily distinguished from free QDs by the absence of blinking.

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Section: Resultssupporting

confidence: 85%

Fluorescence Blinking as an Output Signal for Biosensing

et al. 2016

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“…24 To date, a variety of distinct NANPs has been demonstrated differing from one another by their shape, size, internal connectivity, and physicochemical properties. 24–47 Ongoing development of NANPs is motivated by their potential applications in medicine and biotechnology, including their uses as carriers for RNAi inducers, 47–50 aptamers, 48, 51 fluorophores, and as customizable materials. 52–54 Striking features of these nanoparticles are their precisely defined molecular structures, chemical stability, and tunable physiochemical properties.…”

mentioning

confidence: 99%

Picomolar Fingerprinting of Nucleic Acid Nanoparticles Using Solid-State Nanopores

Alibakhshi¹,

Halman²,

Wilson

et al. 2017

ACS Nano

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Nucleic acid nanoparticles (NANPs) are an emerging class of programmable structures with tunable shape and function. Their promise as tools for fundamental biophysics studies, molecular sensing, and therapeutic applications necessitates methods for their detection and characterization at the single-particle level. In this work, we study electrophoretic transport of individual ringCorresponding Authors, aksiment@illinois.edu, kafonin@uncc.edu, wanunu@neu.edu. ORCID, Aleksei Aksimentiev: 0000-0002-6042-8442 Meni Wanunu: 0000-0002-9837-0004 ¶ M.A.A., J.RH., and J.W. contributed equally to this work.The authors declare no competing financial interest. ASSOCIATED CONTENT

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“…Nucleic acids, beyond the native function for RNA interference 43–45 , enzyme-like activity 46 , DNA repair 47 , and other genome editing 48 , can also be designed and constructed with defined shape and structure 49,50 . Nucleic acid nanoparticles have great potential to overcome the liver accumulation limitations for in vivo application 23,24,27,28,31,51 , with the nature of negative surface charge and well defined particle shape and size. The multivalent nature of the pRNA-3WJ allows us to conjugate multiple molecules with different functions.…”

Section: Discussionmentioning

confidence: 99%

RNA nanoparticles harboring annexin A2 aptamer can target ovarian cancer for tumor-specific doxorubicin delivery

Zhang

et al. 2017

Nanomedicine: Nanotechnology, Biology and Medicine

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A novel modified nucleic acid nanoparticle harboring an annexin A2 aptamer for ovarian cancer cell targeting and a GC rich sequence for doxorubicin loading is designed and constructed. The system utilizes a highly stable three-way junction (3WJ) motif from phi29 packaging RNA as a core structure. A phosphorothioate-modified DNA aptamer targeting annexin A2, Endo28, was conjugated to one arm of the 3WJ. The pRNA-3WJ motif retains correct folding of attached aptamer, keeping its functions intact. It is of significant utility for aptamer-mediated targeted delivery. The DNA/RNA hybrid nanoparticles remained intact after systemic injection in mice and strongly bound to tumors with little accumulation in healthy organs 6 hours post-injection. The Endo28-3WJ-Sph1/Dox intercalates selectively enhanced toxicity to annexin A2 positive ovarian cancer cells in vitro. The constructed RNA/DNA hybrid nanoparticles can potentially enhance the therapeutic efficiency of doxorubicin at low doses for ovarian cancer treatment through annexin A2 targeted drug delivery.

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Controllable Self‐Assembly of RNA Tetrahedrons with Precise Shape and Size for Cancer Targeting

Cited by 81 publications

References 45 publications

Fluorescence Blinking as an Output Signal for Biosensing

Fluorescence Blinking as an Output Signal for Biosensing

Picomolar Fingerprinting of Nucleic Acid Nanoparticles Using Solid-State Nanopores

RNA nanoparticles harboring annexin A2 aptamer can target ovarian cancer for tumor-specific doxorubicin delivery

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