Controllable preparation of bioactive open porous microspheres for tissue engineering

Lin, Anqi; Liu, Shengyang; Xiao, Lan; Fu, Yingying; Liu, Changsheng; Li, Yulin

doi:10.1039/d2tb01198k

Cited by 7 publications

(5 citation statements)

References 51 publications

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“…Uniform and appropriate particle size may affect cell adhesion and proliferation. [3,46] The MSs made at 1500 rpm rotation speed are the most suitable size for cell culture, as their water content is the highest and is able to provide oxygen and nutrients to the cells.…”

Section: Discussionmentioning

confidence: 99%

Nanoclay‐Modified Hyaluronic Acid Microspheres for Bone Induction by Sustained rhBMP‐2 Delivery

Zhang

Sun

et al. 2023

Macromolecular Bioscience

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Microspheres (MSs) were ideal candidates as biological scaffolds loading with growth factors or cells for bone tissue engineering to repair irregular alveolar bone defects by minimally invasive injection. However, the high initial burst release of growth factor and low cell attachment limit the application of microspheres. The modification of microspheres often needs expensive experiments facility or complex chemical reactions, which is difficult to achieve and may bring other problems. In this study, a sol‐grade nanoclay, laponite XLS is used to modify the surface of MSs to enhance its affinity to either positively or negatively charged proteins and cells without changing the interior structure of the MSs. Recombinant human bone morphogenetic protein‐2 (rhBMP‐2) was used as a representation of growth factor to check the osteoinduction ability of laponite XLS‐modified MSs. By modification, the protein sustained release, cell loading, and osteoinduction ability of MSs have been improved. Modified by 1% laponite XLS, the MSs could not only promote osteogenic differentiation of MC3T3‐E1 cells by themselves but also enhance the effect of the rhBMP‐2 below the effective dose. Collectively, the study provides an easy and viable method to modify the biological behavior of microspheres for bone tissue regeneration.This article is protected by copyright. All rights reserved

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Section: Discussionmentioning

confidence: 99%

Nanoclay‐Modified Hyaluronic Acid Microspheres for Bone Induction by Sustained rhBMP‐2 Delivery

Zhang

Sun

et al. 2023

Macromolecular Bioscience

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“…1a. The porosity of MSW20 is denser than that of WP and MSW10, and a denser porosity facilitates the spread of molecules and the exchange of nutrients and waste products inside composites [37]. Hydrogels with three-dimensional network structures can swell in water and avoid autolysis.…”

Section: Morphology and Analysis Of The Compositionmentioning

confidence: 99%

The Effect of a Wheat Protein-based Magnesium Silicate Hydrogel Loaded with a Glucosamine Composite on the Regeneration of Cartilage

Zhang,

Yang,

et al. 2024

Preprint

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How to treat cartilage defects effectively in the clinic has become a great challenge. Due to the lack of blood vessels, nerves and the lymphatic system, self-repair of cartilage usually cannot be achieved when damaged. Therefore, identifying a potential therapeutic method for cartilage regeneration is necessary. In the present study, wheat protein (WP)-based hydrogels loaded with magnesium silicate (MS) and glucosamine (GA) were fabricated via the sol-gel method, and the restorative effect of the resulting hydrogel composite (MSWG) on cartilage defects was also evaluated. The mechanical strength, biodegradability and hydrophilicity of MSW have been largely improved. Moreover, the porous structure of MSWG promotes cell adhesion, proliferation and migration. MSWG promotes the expression of genes and proteins related to chondrogenic differentiation in vitro To evaluate the effect of MSWG on cartilage regeneration in vivo, rabbit knee cartilage defects were established in this study, and MSWG composites were implanted into the defects. More cartilage and subchondral bone appeared around the defect in the MSWG group, and the chondrocytes seemed to mature. In conclusion, the addition of GA to MSW improved its physicochemical properties and biocompatibility. The synergistic effect of hydrogel composites has been proven to promote the expression of chondrogenic biomarkers and accelerate the repair of cartilage defects. MSWG hydrogel composites, as potential candidates, are promising for application in cartilage regeneration.

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“…In recent years, various studies on the diameter and number of pores of microspheres have attracted increasing attentions [28,29]. The early study by Hulbert et al reported that the minimal pore size for bone regeneration was in the range of 75-100 µm [30].…”

Section: Characteristics Types and Compositions Of Microspheresmentioning

confidence: 99%

Progress in microsphere-based scaffolds in bone/cartilage tissue engineering

Pan,

Su,

Yao

2023

Biomed. Mater.

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Bone/cartilage repair and regeneration have been popular and difficult issues in medical research. Tissue engineering is rapidly evolving to provide new solutions to this problem, and the key point is to design the appropriate scaffold biomaterial. In recent years, microsphere-based scaffolds have been considered suitable scaffold materials for bone/cartilage injury repair because microporous structures can form more internal space for better cell proliferation and other cellular activities, and these composite scaffolds can provide physical/chemical signals for neotissue formation with higher efficiency. This paper reviews the research progress of microsphere-based scaffolds in bone/chondral tissue engineering, briefly introduces types of microspheres made from polymer, inorganic and composite materials, discusses the preparation methods of microspheres and the exploration of suitable microsphere pore size in bone and cartilage tissue engineering, and finally details the application of microsphere-based scaffolds in biomimetic scaffolds, cell proliferation and drug delivery systems.

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Controllable preparation of bioactive open porous microspheres for tissue engineering

Abstract: Biodegradable microspheres have been widely applied as cell carriers for tissue engineering and regenerative medicine. However, most cell carriers only have simple planar structure and show poor biological activity and...

Cited by 7 publications

References 51 publications

Nanoclay‐Modified Hyaluronic Acid Microspheres for Bone Induction by Sustained rhBMP‐2 Delivery

Nanoclay‐Modified Hyaluronic Acid Microspheres for Bone Induction by Sustained rhBMP‐2 Delivery

The Effect of a Wheat Protein-based Magnesium Silicate Hydrogel Loaded with a Glucosamine Composite on the Regeneration of Cartilage

Progress in microsphere-based scaffolds in bone/cartilage tissue engineering

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