Controllable microfluidic production of multicomponent multiple emulsions

Wang, Wei; Xie, Rui; Ju, Xin; Luo, Tao; Liu, Li; Weitz, David A.; Chu, Liang‐Yin

doi:10.1039/c1lc20065h

Cited by 208 publications

(161 citation statements)

References 32 publications

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“…Controllable complex multiple emulsions fabricated in microfluidics offer perfect templates to assemble monodisperse structured liposomes with distinct internal compartments. 72 To prove this concept, we upgraded the microfluidic device with two independent droplet generators at the first stage as shown in Figures 6a and S17. The two droplet streams, of which one contains Alexa Fluor 488 dye (W1) and the other contains Alexa Fluor 647 dye (W1'), were paired in a larger micro-capillary (Movies S5) and encapsulated into a larger droplet at the second stage to form double emulsions containing different droplets (Figures 6b, S17 and Movie S6).…”

mentioning

confidence: 99%

Monodisperse Uni- and Multicompartment Liposomes

Deng¹,

Yelleswarapu²,

Huck³

2016

J. Am. Chem. Soc.

225

228

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Liposomes are self-assembled phospholipid vesicles with great potential in fields ranging from targeted drug delivery to artificial cells. The formation of liposomes using microfluidic techniques has seen considerable progress, but the liposomes formation process itself has not been studied in great detail. As a result, high throughput, high-yielding routes to monodisperse liposomes with multiple compartments have not been demonstrated. Here, we report on a surfactant-assisted microfluidic route to uniform, single bilayer liposomes, ranging from 25 µm to 190 µm, and with or without multiple inner compartments. The key of our method is the precise control over the developing interfacial energies of complex W/O/W emulsion systems during liposome formation, which is achieved via an additional surfactant in the phase. The liposomes consist of single bilayers, as demonstrated by nanopore formation experiments and confocal fluoresce microscopy, and they can act as compartments for cell-free gene expression. The microfluidic technique can be expanded to create liposomes with a multitude of coupled compartments, opening routes to networks of multistep microreactors.There has been a significant interest in the use of liposomes, self-assembled phospholipid vesicles composed of bilayer membranes, in fields as diverse as targeted drug delivery, 1,2 membrane protein science, 3-5 bioreactors 6-8 and biosensors. 9,10 Cell-sized liposomes that encapsulate biomolecules and incorporate biological functions provide a versatile mimic of certain aspects of living cells, as exemplified by work showing RNA replication, [11][12][13] in vitro transcription and translation of gene networks, 6,14-16 and organization of cell division machinery in liposomes. [17][18][19][20][21][22][23][24]

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mentioning

confidence: 99%

Monodisperse Uni- and Multicompartment Liposomes

Deng¹,

Yelleswarapu²,

Huck³

2016

J. Am. Chem. Soc.

225

228

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“…Microfluidic emulsification devices such as planar and non-planar junctions, flow focusing devices and glass capillary devices can generate double, triple, quadruple, and quintuple multiple emulsion drops with a polydispersity in the dripping regime of less than (Higashi et al, 1999(Higashi et al, , 1995(Higashi et al, , 2000 SPG (repeated PME, n=5-6) 10.7 mm (b) Core-shell droplets (Vladisavljević et al, 2012a); (c) multiple concentric shells (Kim et al, 2011a); (d) Drops with controlled number of inner droplets (2, 3, 4, …) ; (e) High-order multiple emulsions with controlled number of droplets at each level ; (e) Core/shell droplets with numerous inner droplets ; (g) Distinct inner droplets (Wang et al, 2011); (h) Janus and ternary droplets (Yang et al, 2012;Nie et al, 2006); (i) Non-spherical Janus droplets (Shepherd et al, 2006). Figure 3.…”

Section: Discussionmentioning

confidence: 99%

“…15c, four separate injection tubes were used to produce barcode particles consisting of four different photonic crystal cores embedded in a single polyethylene glycol shell (Zhao et -24-al., 2012). Multiple emulsion droplets with multiple cores can be also generated using glass capillary devices with sequential drop generation in co-flowing streams (Wang et al, 2011). Figure 1h) Nisisako et al (2006) produced Janus droplets in a quartz glass microfluidic chip consisting of two junctions: upstream Y-junction was used to form a two-phase organic stream, which was disintegrated into Janus drops in a downstream cross junction (Fig.…”

Section: Multiple Emulsion Drops With Distinct Inner Drops (Figure 1g)mentioning

confidence: 99%

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Recent advances in the production of controllable multiple emulsions using microfabricated devices

Vladisavljević

2016

Particuology

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This review focuses on recent developments in fabrication of multiple emulsions in microscale systems, such as membrane, microchannel array and microfluidic emulsification devices.Membrane and microchannel emulsification offer great potential in manufacturing multiple emulsions with uniform drop sizes and high encapsulation efficiency of encapsulated actives.Microfluidic devices enable unprecedented level of control over the number, size and type of internal droplets at each hierarchical level but suffer from low production scales. Microfluidic methods can be exploited to generate high-order multiple emulsions (triple, quadruple and quintuple), non-spherical (discoidal and rod-like) drops and drops with asymmetric properties such as Janus and ternary drops, composed of two or three distinct regions on the surface.Multiple emulsion droplets generated in microfabricated devices can be used as templates for vesicles (polymersomes, liposomes, colloidosomes) with multiple inner compartments for simultaneous encapsulation and release of incompatible actives or reactants.-2-

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“…[20][21][22] By confining precursor solutions in differently shaped microwells of molds, nonspherical microparticles with flexible shapes can also be produced. [23,24] With excellent manipulation of microflows, [28][29][30] microfluidic technique provides a powerful platform for fabricating nonspherical microparticles with versatile structures and compositions. [26,31,32] Mask-assisted selective polymerization of photo-curable solution in microchannel can fabricate nonspherical microparticles with flexible shapes depending on their mask, [25,33] and the flow configuration.…”

Section: Doi: 101002/marc201700429mentioning

confidence: 99%

Controllable Microfluidic Fabrication of Microstructured Materials from Nonspherical Particles to Helices

Wang

Zhang

et al. 2017

Macromol. Rapid Commun.

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delivery, [1] drug release, [8] assembling, [9,10] packing, [11,12] and magnetic-driven motion. [13,14] For example, cylindrical microstructured materials self-assembled from copolymers can persist in the blood circulation of rodents ten times longer than their spherical counterparts. [2] Helical microstructured materials can convert rotational motion into translational motion in liquid media, such as water and blood, under remote control of a rotated magnetic field, showing great potential for many applications. [13,15] With such unique motion behaviors, helical microstructured materials can be utilized for uses such as picking and placing of microcargo, [14] mixing and pumping of fluid, [16] remote sensing of pH, [17] drilling of bovine tissues, [18] and cancer hyperthermia. [19] Thus, creation of uniform microstructured materials with versatile nonspherical and helical shapes is crucial for achieving advanced functions.Generally, microstructured materials with nonspherical and helical shapes can be produced by methods such as film-based extension of spherical microparticles, [20][21][22] mold-based template synthesis, [23,24] microfluidic-based template synthesis, [8,[25][26][27] and polymerization induced by 3D direct laser writing. [14] For example, incorporation of polystyrene-based microparticles in polymeric film, followed with 1D or 2D film extension, can produce various nonspherical microparticles. [20][21][22] By confining precursor solutions in differently shaped microwells of molds, nonspherical microparticles with flexible shapes can also be produced. [23,24] With excellent manipulation of microflows, [28][29][30] microfluidic technique provides a powerful platform for fabricating nonspherical microparticles with versatile structures and compositions. [26,31,32] Mask-assisted selective polymerization of photo-curable solution in microchannel can fabricate nonspherical microparticles with flexible shapes depending on their mask, [25,33] and the flow configuration. [34,35] Alternatively, monodisperse emulsion droplets from microfluidics, with versatile shapes, provide excellent templates for engineering uniform nonspherical microparticles. Deformed droplets confined by microchannel with different dimensions, [26,27] Janus droplets with one curable hemisphere, [31,36] and evolved acorn-shaped droplets from double emulsions, [32] can be generated from microfluidics for fabricating nonspherical microparticles with different shapes. However, for the above-mentioned methods, it is difficult to produce microstructured materials with complex Microstructured MaterialsThis work reports on a facile and flexible strategy based on the deformation of encapsulated droplets in fiber-like polymeric matrices for template synthesis of controllable microstructured materials from nonspherical microparticles to complex 3D helices. Monodisperse droplets generated from microfluidics are encapsulated into crosslinked polymeric networks via an interfacial crosslinking reaction in microchannel to in situ produce the droplet-...

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Controllable microfluidic production of multicomponent multiple emulsions

Cited by 208 publications

References 32 publications

Monodisperse Uni- and Multicompartment Liposomes

Monodisperse Uni- and Multicompartment Liposomes

Recent advances in the production of controllable multiple emulsions using microfabricated devices

Controllable Microfluidic Fabrication of Microstructured Materials from Nonspherical Particles to Helices

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