Controllable gold nanoparticle deposition on carbon nanotubes and their application in immunosensing

Abstract

A CNT-AuNPs hybrid nanocomposite platform was prepared from nanodisperse AuNPs in N- [3-(trimethoxysilyl)propyl]ethylenediamine (EDAS) sol-gel matrices with purified MWCNT. EDAS, an amine group-containing sol-gel solution, was utilized for its ability to stabilize the nanoparticles in solution. The developed model system was based on immobilized rabbit anti-mouse IgG-HRP (horseradish peroxidase) for reagentless detection of mouse IgG. The immunosensing platform was prepared by using Nafion for the immobilization of rabbit anti-mouse IgG-HRP and CNT-AuNPs hybrid nanocomposite on a glassy carbon electrode used for the detection of mouse IgG which provides a biocompatible microenvironment. The resulting CNT-AuNPs nanocomposite brings new capabilities for electrochemical devices by using the synergistic action of its electrocatalytic activity. The CNT-AuNPs were characterized using SEM, TEM, EIS, and AFM, and the analytical performance was monitored by differential pulse voltammetry. The detection limit of mouse IgG is 0.5 ng mL À1 (S/N ratio ¼ 3). In addition, the immunosensor efficiently allowed a specific electrochemical analysis of mouse IgG and easy discrimination of goat IgG, chicken IgG, and rabbit IgG.

New approach of creation and modifying the characteristics of AuNPs/MWCNT/PSi hybrid structures

“…Hydrophobic, stacking, electrostatic [32][33][34], and other mechanisms are used in the non-covalent adsorption and Van der Waals interactions [35] between the AuNPs that are shielded by a monolayer and the native or treated CNT. This method is can preserves the stability of the CNT sidewalls' chemical composition [36][37][38][39]. In this work, a new path-way is suggested as a simple synthesis modality that can improve the characteristics of the AuNPs/MWCNT/PSi hybrid structure by controlling the pores dimensions, dangling bonds with ions reduction process to decorate the MWCNT and modify the (S.S.A) of the hybrid structure.…”

Immunosensor for prostate-specific antigen using Au/Pd@flower-like SnO2 as platform and Au@mesoporous carbon as signal amplification

“…8 Among these strategies, nanomaterial-based strategies have potential in realizing ultrasensitive biological detection due to the versatile properties of nanomaterials. 9 Based on this concept, many researchers choose some carbon materials such as carbon nanotubes, 4,8,10 carbon nanohorns, 11 graphene, [12][13][14] mesoporous carbons, 15,16 and fullerene 17 as efficient carriers. Among these carbon materials, mesoporous carbon is an excellent material for applications in catalysis, sensing and energy storage due to its unique features, such as good electrical conductivity, good biocompatibility, excellent adsorption properties, large specic surface area, high pore volume, and tunable porosity.…”