Controllable dual‐release of dexamethasone and bovine serum albumin from PLGA/β‐tricalcium phosphate composite scaffolds

Abstract

Localized dual-drug delivery from biodegradable scaffolds is an important strategy in tissue engineering. In this study, porous poly(L-lactide-co-glycolide) (PLGA)/β-tricalcium phosphate scaffolds containing both dexamethasone (Dex) and bovine serum albumin (BSA) were prepared by incorporating Dex-loaded and BSA-loaded microspheres into the scaffolds. PLGA microspheres containing Dex or BSA were prepared by spray-drying and double emulsion/solvent evaporation, respectively. In vitro release studies indicated that microspheres prepared from PLGA in 3:1 molar ratio of L-lactide/glycolide and 89.5 kDa relative molecular mass showed prolonged release profiles compared with those prepared from PLGA in 1:1 L-lactide/glycolide molar ratio and 30.5 kDa relative molecular mass. Additionally, introduction of poly(ethylene glycol) in the PLGA chain could improve the encapsulation efficiency and reduce the release rate. Based on the above results, controllable dual-release of Dex and BSA with relatively higher or lower release rate was achieved by incorporating Dex-loaded and BSA-loaded microspheres with different release profiles into the PLGA/β-tricalcium phosphate scaffolds.

Controlled release of clarithromycin from PLGA microspheres enhances bone regeneration in rabbit calvaria defects

“…In previous studies, it was reported that the incorporation of bone substitutes with microspheres demonstrated an initial burst release in the first days, which was followed by slower release kinetics as the microspheres biodegraded . In the current study, the release profile suggested that the microspheres released around 50% of clarithromycin during the first week.…”

Poly(lactide- co -glycolide) porous scaffolds for tissue engineering and regenerative medicine

“…Drugs or proteins especially growth factors or genes to express growth factors were also loaded into porous scaffolds, which could significantly improve the regeneration of new tissues [12,55,75,83,84].…”

Protein encapsulated in electrospun nanofibrous scaffolds for tissue engineering applications

“…The electrospun scaffold possesses high specific surface area, high porosity and an interconnected pore network, which is similar architecturally to the natural ECM and can enhance the adhesion and growth of cells . The development of a porous scaffold, capable of controlling local delivery of bioactive agents, to promote cell behavior and facilitate tissue restoration is one of the major purposes of tissue engineering …”