Controllable cell manipulation in a microfluidic pipette-tip design using capacitive coupling of electric fields

Wimberger, Terje; Peham, Johannes R.; Ehmoser, Eva-Kathrin; Wassermann, Klemens J.

doi:10.1039/c9lc00927b

Cited by 7 publications

(4 citation statements)

References 33 publications

(39 reference statements)

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“…TiO 2 has also been proposed as a suitable dielectric material for titanium electrodes. ,, This layer limits the passage of off-target currents through the electrolyte solution. Wimberger et al found that passivated electrodes led to more consistent cell inactivation at different electric field strengths than exposed electrodes because stochastic electrolysis effects were minimized (Figure A) . 18 μm thin layers of PDMS have also been used as a passivating layer for interdigitated electrodes .…”

Section: Technological Improvementsmentioning

confidence: 99%

Section: Technological Improvementsmentioning

confidence: 99%

“…Wimberger et al found that passivated electrodes led to more consistent cell inactivation at different electric field strengths than exposed electrodes because stochastic electrolysis effects were minimized (Figure 13A). 262 18 μm thin layers of PDMS have also been used as a passivating layer for interdigitated electrodes. 263 The PDMS layer protected electrodes under conditions as extreme as an applied DC voltage of 800 V for several hours (Figure 13B).…”

Section: General Advancesmentioning

confidence: 99%

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Recent Advances in Microscale Electroporation

2022

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Electroporation (EP) is a commonly used strategy to increase cell permeability for intracellular cargo delivery or irreversible cell membrane disruption using electric fields. In recent years, EP performance has been improved by shrinking electrodes and device structures to the microscale. Integration with microfluidics has led to the design of devices performing static EP, where cells are fixed in a defined region, or continuous EP, where cells constantly pass through the device. Each device type performs superior to conventional, macroscale EP devices while providing additional advantages in precision manipulation (static EP) and increased throughput (continuous EP). Microscale EP is gentle on cells and has enabled more sensitive assaying of cells with novel applications. In this Review, we present the physical principles of microscale EP devices and examine design trends in recent years. In addition, we discuss the use of reversible and irreversible EP in the development of therapeutics and analysis of intracellular contents, among other noteworthy applications. This Review aims to inform and encourage scientists and engineers to expand the use of efficient and versatile microscale EP technologies.

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Section: Technological Improvementsmentioning

confidence: 99%

Section: Technological Improvementsmentioning

confidence: 99%

Section: General Advancesmentioning

confidence: 99%

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Recent Advances in Microscale Electroporation

2022

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“…Boukany et al utilized a micro-nano-micro-channel for the precise delivery of transfection agents to the trapped single cells [ 27 ]. In single-cell electroporation systems, complicated micro/nano-fluidic channels [ 26 , 27 , 28 , 29 ] or single-cell micromanipulation units [ 30 , 31 , 32 , 33 ] were usually required. In addition, most of these systems were combined with an optical detection tool for visualizing electroporation results [ 34 ].…”

Section: Introductionmentioning

confidence: 99%

Single-Cell Electroporation with Real-Time Impedance Assessment Using a Constriction Microchannel

Luan

Zhang

et al. 2020

Micromachines

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The electroporation system can serve as a tool for the intracellular delivery of foreign cargos. However, this technique is presently limited by the inaccurate electric field applied to the single cells and lack of a real-time electroporation metrics subsystem. Here, we reported a microfluidic system for precise and rapid single-cell electroporation and simultaneous impedance monitoring in a constriction microchannel. When single cells (A549) were continuously passing through the constriction microchannel, a localized high electric field was applied on the cell membrane, which resulted in highly efficient (up to 96.6%) electroporation. During a single cell entering the constriction channel, an abrupt impedance drop was noticed and demonstrated to be correlated with the occurrence of electroporation. Besides, while the cell was moving in the constriction channel, the stabilized impedance showed the capability to quantify the electroporation extent. The correspondence of the impedance variation and electroporation was validated by the intracellular delivery of the fluorescence indicator (propidium iodide). Based on the obtained results, this system is capable of precise control of electroporation and real-time, label-free impedance assessment, providing a potential tool for intracellular delivery and other biomedical applications.

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Soft‐Contact Acoustic Microgripper Based on a Controllable Gas–Liquid Interface for Biomicromanipulations

Zhou

Liu

Yan

et al. 2021

Small

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engineering, [2] drug delivery, [3] and biomedical research. [4] Until now, micromanipulation methods based on direct physical contact have been used widely.To achieve capture and transportation, different end-effectors have been introduced into systems, such as a microgripper for direct grasping [5] or driven by negative pressure [6] and a cantilever. [7] Through visual feedback, micromanipulation can be realized efficiently and effectively. However, direct contact between solid manipulation tools and bioentities may cause potential damage to living cells that would be difficult to observe by the naked eye. [8] Despite the potential damage to living cells during the manipulation process, the release of micro-objects also poses a significant challenge because of the adhesion force at the microscale due to direct physical contact.The ORCID identification number(s) for the author(s) of this article can be found under https://doi.org/10.1002/smll.202104579.

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Controllable cell manipulation in a microfluidic pipette-tip design using capacitive coupling of electric fields

Abstract: Capacitive coupling of electric fields diminishes energy dissipation and offers superior control over field parameters, resulting in predictable biological outcomes.

Cited by 7 publications

References 33 publications

Recent Advances in Microscale Electroporation

Recent Advances in Microscale Electroporation

Single-Cell Electroporation with Real-Time Impedance Assessment Using a Constriction Microchannel

Soft‐Contact Acoustic Microgripper Based on a Controllable Gas–Liquid Interface for Biomicromanipulations

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