Controllable biogenic synthesis of intracellular silver/silver chloride nanoparticles by Meyerozyma guilliermondii KX008616

Alamri, Saad; Hashem, Mohamed; Nafady, Nivien A.; Sayed, M. A.; Alshehri, A.M.; Alshaboury, Gamal A.

doi:10.4014/jmb.1802.02010

Cited by 22 publications

(3 citation statements)

References 50 publications

(54 reference statements)

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“…AgCl-NPs were produced due to presence of NaCl in medium. Synthesis of AgCl-NPs from a silver nitrate precursor as mediated by supernatant of several microbes has been reported as the fungi such as Macrophomina phaseolina [ 28 ], yeasts such as Meyerozyma guilliermondii KX008616 [ 29 ], and bacteria such as Raoultella planticola and Pantoea agglomerans [ 30 ]. However, the silver chloride nanoparticles synthesis by supernatant of bacteria B. mojavensis was first reported.…”

Section: Discussionmentioning

confidence: 99%

Biosynthesis of Silver Chloride Nanoparticles by Rhizospheric Bacteria and Their Antibacterial Activity against Phytopathogenic Bacterium Ralstonia solanacearum

Alamer

Tomah

et al. 2021

Molecules

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Ralstonia solanacearum is the most destructive pathogen, causing bacterial wilt disease of eggplant. The present study aimed to develop green synthesis and characterization of silver chloride nanoparticles (AgCl-NPs) by using a native bacterial strain and subsequent evaluation of their antibacterial activity against R. solanacearum. Here, a total of 10 bacterial strains were selected for the biosynthesis of AgCl-NPs. Among them, the highest yield occurred in the synthesis of AgCl-NPs using a cell-free aqueous filtrate of strain IMA13. Ultrastructural observation revealed that the AgCl-NPs were spherical and oval with smooth surfaces and 5–35 nm sizes. XRD analysis studies revealed that these particles contained face-centered cubic crystallites of metallic Ag and AgCl. Moreover, FTIR analysis showed the presence of capping proteins, carbohydrates, lipids, and lipopeptide compounds and crystalline structure of AgCl-NPs. On the basis of phylogenetic analysis using a combination of six gene sequences (16S, gyrA, rpoB, purH, polC, and groEL), we identified strain IMA13 as Bacillus mojavensis. Three kinds of lipopeptide compounds, namely, bacillomycin D, iturin, and fengycin, forming cell-free supernatant produced by strain IAM13, were identified by MALDI-TOF mass spectrometry. Biogenic AgCl-NPs showed substantial antibacterial activity against R. solanacearum at a concentration of 20 µg/mL−1. Motility assays showed that the AgCl-NPs significantly inhibited the swarming and swimming motility (61.4 and 55.8%) against R. solanacearum. Moreover, SEM and TEM analysis showed that direct interaction of AgCl-NPs with bacterial cells caused rupture of cell wall and cytoplasmic membranes, as well as leakage of nucleic acid materials, which ultimately resulted in the death of R. solanacearum. Overall, these findings will help in developing a promising nanopesticide against phytopathogen plant disease management.

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Section: Discussionmentioning

confidence: 99%

Biosynthesis of Silver Chloride Nanoparticles by Rhizospheric Bacteria and Their Antibacterial Activity against Phytopathogenic Bacterium Ralstonia solanacearum

Alamer

Tomah

et al. 2021

Molecules

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“…Some microorganisms are able to accumulate and detoxify heavy metals due to various reductase enzymes, which reduce metal salts to metal nanoparticles with a narrow size distribution. For this reason, microorganisms have immense potential as ecofriendly and cost-effective tools for biological synthesis of nanoparticles, avoiding toxic, harsh chemicals and the high energy demand required for physiochemical synthesis [2] Biological synthesis by microorganisms includes the intracellular or extracellular production [3,4]. Extracellular synthesis of nanoparticles has received much attention because it eliminates the downstream processing steps required for the recovery of nanoparticles in intracellular methodologies, such as sonication to break microscopy (ESEM), and confocal Raman microscopy (CRM).…”

Section: Introductionmentioning

confidence: 99%

Biofilm Eradication Using Biogenic Silver Nanoparticles

et al. 2020

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Microorganisms offer an alternative green and scalable technology for the synthesis of value added products. Fungi secrete high quantities of bioactive substances, which play dual-functional roles as both reducing and stabilizing agents in the synthesis of colloidal metal nanoparticles such as silver nanoparticles, which display potent antimicrobial properties that can be harnessed for a number of industrial applications. The aim of this work was the production of silver nanoparticles using the extracellular cell free extracts of Phanerochaete chrysosporium, and to evaluate their activity as antimicrobial and antibiofilm agents. The 45–nm diameter silver nanoparticles synthesized using this methodology possessed a high negative surface charge close to −30 mV and showed colloidal stability from pH 3–9 and under conditions of high ionic strength ([NaCl] = 10–500 mM). A combination of environmental SEM, TEM, and confocal Raman microscopy was used to study the nanoparticle-E. coli interactions to gain a first insight into their antimicrobial mechanisms. Raman data demonstrate a significant decrease in the fatty acid content of E. coli cells, which suggests a loss of the cell membrane integrity after exposure to the PchNPs, which is also commensurate with ESEM and TEM images. Additionally, these biogenic PchNPs displayed biofilm disruption activity for the eradication of E. coli and C. albicans biofilms.

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“…Some microorganisms are able to accumulate and detoxify heavy metals due to various reductase enzymes, which reduce metal salts to metal nanoparticles with a narrow size distribution. For this reason, microorganisms have immense potential as ecofriendly and cost-effective tools for biological synthesis of nanoparticles, avoiding toxic, harsh chemicals and the high energy demand required for physiochemical synthesis [2] Biological synthesis by microorganisms includes the intracellular or extracellular production [3,4]. Extracellular synthesis of nanoparticles has received much attention because it eliminates the downstream processing steps required for the recovery of nanoparticles in intracellular methodologies, such as sonication to break down the cell wall and several centrifugation and washing steps required for nanoparticle purification.…”

Section: Introductionmentioning

confidence: 99%

Biogenic silver nanoparticles against Escherichia coli planktonic and biofilm cells

Estevez¹,

Raffaelli²,

Mitchell³

et al. 2020

Preprint

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Microorganisms offer an alternative green and scalable technology for the synthesis of value added products. Fungi secrete high quantities of bioactive substances, which play dual-functional roles as both reducing and stabilizing agents in the synthesis of colloidal metal nanoparticles such as silver nanoparticles, which display potent antimicrobial properties that can be harnessed for a number of industrial applications. The aim of this work was the production of silver nanoparticles using the extracellular cell free extracts of Phanerochaete chrysosporium, and to evaluate their activity as antimicrobial and antibiofilm agents. The 45-nm diameter silver nanoparticles synthesized using this methodology possessed a high negative surface charge close to −30 mV and showed colloidal stability from pH 3-9 and under conditions of high ionic strength ([NaCl] = 10-500 mM). A combination of environmental SEM, TEM, and confocal Raman microscopy was used to study the nanoparticle-E. coli interactions to gain a first insight into their antimicrobial mechanisms. Raman data demonstrate a significant decrease in the fatty acid content of E. coli cells, which suggests a loss of the cell membrane integrity after exposure to the PchNPs, which is also commensurate with ESEM and TEM images. Additionally, these biogenic PchNPs displayed biofilm disruption activity for the eradication of E. coli and C. albicans biofilms.

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Controllable biogenic synthesis of intracellular silver/silver chloride nanoparticles by Meyerozyma guilliermondii KX008616

Cited by 22 publications

References 50 publications

Biosynthesis of Silver Chloride Nanoparticles by Rhizospheric Bacteria and Their Antibacterial Activity against Phytopathogenic Bacterium Ralstonia solanacearum

Biosynthesis of Silver Chloride Nanoparticles by Rhizospheric Bacteria and Their Antibacterial Activity against Phytopathogenic Bacterium Ralstonia solanacearum

Biofilm Eradication Using Biogenic Silver Nanoparticles

Biogenic silver nanoparticles against Escherichia coli planktonic and biofilm cells

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