Control strategy of pH, dissolved oxygen concentration and stirring speed for enhancing β‐poly (malic acid) production by <i>Aureobasidium pullulans</i> ipe‐1

Cao, Weifeng; Qi, Benkun; Zhao, Juan; Qiao, Changsheng; Su, Yi; Wan, Yinhua

doi:10.1002/jctb.3905

Cited by 36 publications

(36 citation statements)

References 39 publications

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“…However, L‐malate was widely verified and accepted as the precursor for PMLA biosynthesis . Meanwhile, a high DO level was beneficial for PMLA production . Therefore, the key enzymes activities involved in the precursor production for PMLA biosynthesis was studied under a high DO level (70% saturation) and a low DO level (10% saturation).…”

Section: Discussionmentioning

confidence: 99%

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Toward understanding the key enzymes involved in β‐poly (L‐malic acid) biosynthesis by Aureobasidium pullulans ipe‐1

Liu

Luo

et al. 2018

Engineering in Life Sciences

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β‐poly (L‐malic acid) (PMLA) is a biopolyester which has attracted industrial interest for its potential application in medicine and other industries. A high dissolved oxygen concentration (DO) was beneficial for PMLA production, while the mechanisms of DO in PMLA biosynthesis by Aureobasidium pullulans are still poorly understood. In this work, the amount of PMLA was first compared when A. pullulans ipe‐1 were cultured under a high DO level (70% saturation) and a low DO level (10% saturation). Meanwhile, the key enzymes involved in different pathways of the precursor L‐malic acid biosynthesis were studied. The results revealed that the activities of glucose‐6‐phosphate dehydrogenase (G6PDH) and phosphoenolpyruvate carboxylase (PEPC) were positively correlated with cell growth and PMLA production, while the activities of phosphofructokinases (PFK), pyruvic carboxylase (PC) and citrate synthetase (CS) did no show such correlations. It indicated that the Pentose Phosphate Pathway (PPP) may play a vital role in cell growth and PMLA biosynthesis. Moreover, the precursor L‐malic acid for PMLA biosynthesis was mainly biosynthesized through phosphoenolpyruvic acid (PEP) via oxaloacetate catalyzed by PEPC. It was also found that low concentration of sodium fluoride (NaF) might impel carbon flux flow to the oxaloacetate through PEP, but inhibit the flux to the oxaloacetate via pyruvic acid.

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Section: Discussionmentioning

confidence: 99%

“…Meanwhile, Cao et al. found that a high dissolved oxygen (DO) level was beneficial for PMLA production. Moreover, other products biosynthesized by A. pullulans , such as pullulan and biosurfactant , were all affected by DO level.…”

Section: Introductionmentioning

confidence: 99%

Toward understanding the key enzymes involved in β‐poly (L‐malic acid) biosynthesis by Aureobasidium pullulans ipe‐1

Liu

Luo

et al. 2018

Engineering in Life Sciences

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“…In the 10-l benchtop fermentor, a high PMA concentration (57.2 g/l) produced by A. pullulans ZD-3 d was achieved within 160 h when the fermentation medium contained 120.0 g/l of glucose and 30.0 g/l of CaCO3 (Zhang et al, 2011). A. pullulans ipe-1 could produce up to 63.2 g/l of PMA during the repeatedbatch cultivation (Cao et al, 2013). A. pullulans strain ZX-10 produced PMA 50.0 g/l during batch fermentation in free-cell fermentation in a stirred-tank bioreactor (Zou et al, 2013).…”

Section: Pma and Its Biosynthesismentioning

confidence: 99%

Taxonomy ofAureobasidiumspp. and biosynthesis and regulation of their extracellular polymers

Chi

Wang

et al. 2013

Critical Reviews in Microbiology

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The genus Aureobasidium spp. have been divided into three species, A. pullulans. A. leucospermi and A. proteae, and A. pullulans has been known to have five varieties. However, after analysis of many strains of this yeast isolated from different environments, they do not belong to any of the three species or the five varieties. Although pullulan produced by A. pullulans has been widely used in different fields in industry and different strains of this yeast has been known to produce poly(β-L-malic acid) (PMA), heavy oils and β-1,3-glucan, it is still unknown how the black yeast synthesizes and secretes the extracellular polymers at molecular level. In this review article, new biosynthetic pathways of pullulan, PMA and heavy oils, the enzymes and their genes related to their biosynthesis and regulation are proposed. Furthermore, some enzymes and their genes related to pullulan biosynthesis in A. pullulans have been characterized. But it is completely unknown how pullulan is secreted and how PMA, heavy oils and β-1,3-glucan are synthesized and secreted. Therefore, there is much work to be done about taxonomy and biosynthesis, secretion and regulation of pullulan, PMA, heavy oils and β-1,3-glucan at molecular levels in Aureobasidium spp.

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“…That means the demand for oxygen in microorganisms varies among different fermentation processes. To ensure the appropriate oxygen supplement, DO is commonly controlled at a suitable level (Cao et al 2013). Take submerged fermentation of Saccharopolyspora spinosa , for example, DO is kept at 40–50% during 0–96 h for cell growth and at 25–35% during 96–240 h for spinosad formation (Bai et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Dissolved oxygen control strategy for improvement of TL1-1 production in submerged fermentation by Daldinia eschscholzii

Wei

Liu

Lü

2017

Bioresour. Bioprocess.

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Background2,3-Dihydro-5-hydroxy-2-methylchromen-4-one (TL1-1) is a phenolic compound with significant anti-fungal and anti-cancer activities produced by Daldinia eschscholzii (D. eschscholzii). However, studies have rarely been reported on the fermentation process of D. eschscholzii due to the urgent demand for its pharmaceutical researches and applications.ResultsIn this work, the optimal fermentation medium for improved TL1-1 yield was first obtained in a shake flask. As the fermentation process was scaling up, the marked effects of dissolved oxygen (DO) on cell growth and TL1-1 biosynthesis were observed and confirmed. Controlling a suitable DO level by the adjustment of agitation speed and aeration rate remarkably enhanced TL1-1 production in a lab-scale bioreactor. Moreover, the fermentation of D. eschscholzii was successfully applied in 500-L bioreactor, and TL1-1 production has achieved 873.63 mg/L, approximately 15.4-fold than its initial production (53.27 mg/L).ConclusionsDissolved oxygen control strategy for enhancing TL1-1 production was first proposed. Furthermore, control of the appropriate DO level has successfully performed for improving TL1-1 yield and scale-up of D. eschscholzii fermentation process.Electronic supplementary materialThe online version of this article (doi:10.1186/s40643-016-0134-4) contains supplementary material, which is available to authorized users.

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Control strategy of pH, dissolved oxygen concentration and stirring speed for enhancing β‐poly (malic acid) production by Aureobasidium pullulans ipe‐1

Cited by 36 publications

References 39 publications

Toward understanding the key enzymes involved in β‐poly (L‐malic acid) biosynthesis by Aureobasidium pullulans ipe‐1

Toward understanding the key enzymes involved in β‐poly (L‐malic acid) biosynthesis by Aureobasidium pullulans ipe‐1

Taxonomy ofAureobasidiumspp. and biosynthesis and regulation of their extracellular polymers

Dissolved oxygen control strategy for improvement of TL1-1 production in submerged fermentation by Daldinia eschscholzii

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