Control over the morphology and segregation of Zebrafish germ cell granules during embryonic development

Strasser, Markus J.; Mackenzie, Natalia C.; Dumstrei, Karin; Nakkrasae, La-iad; Stebler, Jürg; Raz, Erez

doi:10.1186/1471-213x-8-58

Cited by 83 publications

(110 citation statements)

References 52 publications

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“…Diffuse cytoplasmic MCP-GFP was never observed in PGCs of uninjected embryos (n=0/101 PGCs, 15 embryos), although one or two small MCP-GFP accumulations were infrequently visible in perinuclear regions (n=19/101 PGCs, 15 embryos), which probably represent nuclear breakdown associated with mitosis, based on nuclear morphology. Similar punctate signals have been observed for nuclear membrane proteins in dividing PGCs in other studies (Strasser et al, 2008).…”

Section: Visualization Of Germ Granule-like Nanos3 Rna Accumulationssupporting

confidence: 71%

Dynamic visualization of transcription and RNA subcellular localization in zebrafish

et al. 2015

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Live imaging of transcription and RNA dynamics has been successful in cultured cells and tissues of vertebrates but is challenging to accomplish in vivo. The zebrafish offers important advantages to study these processes -optical transparency during embryogenesis, genetic tractability and rapid development. Therefore, to study transcription and RNA dynamics in an intact vertebrate organism, we have adapted the MS2 RNA-labeling system to zebrafish. By using this binary system to coexpress a fluorescent MS2 bacteriophage coat protein (MCP) and an RNA of interest tagged with multiple copies of the RNA hairpin MS2-binding site (MBS), live-cell imaging of RNA dynamics at single RNA molecule resolution has been achieved in other organisms. Here, using a Gatewaycompatible MS2 labeling system, we generated stable transgenic zebrafish lines expressing MCP, validated the MBS-MCP interaction and applied the system to investigate zygotic genome activation (ZGA) and RNA localization in primordial germ cells (PGCs) in zebrafish. Although cleavage stage cells are initially transcriptionally silent, we detect transcription of MS2-tagged transcripts driven by the βactin promoter at ∼3-3.5 h post-fertilization, consistent with the previously reported ZGA. Furthermore, we show that MS2-tagged nanos3 3′UTR transcripts localize to PGCs, where they are diffusely cytoplasmic and within larger cytoplasmic accumulations reminiscent of those displayed by endogenous nanos3. These tools provide a new avenue for live-cell imaging of RNA molecules in an intact vertebrate. Together with new techniques for targeted genome editing, this system will be a valuable tool to tag and study the dynamics of endogenous RNAs during zebrafish developmental processes.

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Section: Visualization Of Germ Granule-like Nanos3 Rna Accumulationssupporting

confidence: 71%

Dynamic visualization of transcription and RNA subcellular localization in zebrafish

et al. 2015

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“…In Drosophila embryos, translocation of VASA-containing granules from the cortex to nuclei occurs along astral microtubules in a process that requires dynein . In zebrafish, microtubules promote germ granule aggregation in early embryos (Theusch et al 2006), and have been implicated in germ granule partitioning during mitosis in primordial germ cells (Strasser et al 2008). Disruption of microtubules or dynein causes the germ granules to coalesce into larger granules, suggesting a role in regulating both granule dynamics and size (Strasser et al 2008).…”

Section: Dynamicsmentioning

confidence: 99%

RNA Granules in Germ Cells

Voronina¹,

Seydoux²,

Sassone–Corsi³

et al. 2011

Cold Spring Harbor Perspectives in Biology

331

354

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“…Similar to several PGC markers in zebrafish (e.g., nanos1, tdrd7, and vasa) (15)(16)(17), the maternally provided mRNA of rgs14a is localized to the cleavage-planes of four-cell embryos, the position where the germ plasm resides (Fig. 1B, Upper) (15,18).…”

Section: Resultsmentioning

confidence: 99%

Temporal control over the initiation of cell motility by a regulator of G-protein signaling

Hartwig

Tarbashevich

Seggewiß³

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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The control over the acquisition of cell motility is central for a variety of biological processes in development, homeostasis, and disease. An attractive in vivo model for investigating the regulation of migration initiation is that of primordial germ cells (PGCs) in zebrafish embryos. In this study, we show that, following PGC specification, the cells can polarize but do not migrate before the time chemokine-encoded directional cues are established. We found that the regulator of G-protein signaling 14a protein, whose RNA is a newly identified germ plasm component, regulates the temporal relations between the appearance of the guidance molecules and the acquisition of cellular motility by regulating E-cadherin levels.rgs protein | cell adhesion

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Control over the morphology and segregation of Zebrafish germ cell granules during embryonic development

Cited by 83 publications

References 52 publications

Dynamic visualization of transcription and RNA subcellular localization in zebrafish

Dynamic visualization of transcription and RNA subcellular localization in zebrafish

RNA Granules in Germ Cells

Temporal control over the initiation of cell motility by a regulator of G-protein signaling

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