Control of Zn uptake in Arabidopsis halleri: a balance between Zn and Fe

Shanmugam, V.; Lo, Jing-Chi; Yeh, Kuo‐Chen

doi:10.3389/fpls.2013.00281

Cited by 56 publications

(39 citation statements)

References 58 publications

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“…B). These results indicated that Fe and Zn concentrations in Arabidopsis shoots inversely change by the concentration of any one of these two nutrients, which is similar to previous reports . Moreover, the concentrations of Mn, Co, Mo, and Cu showed similar varying tendencies to Zn, namely their concentrations were increased by Fe deficiency (Supporting Information Table 1).…”

supporting

confidence: 90%

Quantitative proteomics of Arabidopsis shoot microsomal proteins reveals a cross-talk between excess zinc and iron deficiency

et al. 2015

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Iron (Fe) deficiency significantly effects plant growth and development. Plant symptoms under excess zinc (Zn) resemble symptoms of Fe-deficient plants. To understand cross-talk between excess Zn and Fe deficiency, we investigated physiological parameters of Arabidopsis plants and applied iTRAQ-OFFGEL quantitative proteomic approach to examine protein expression changes in microsomal fraction from Arabidopsis shoots under those physiological conditions. Arabidopsis plants manifested shoot inhibition and chlorosis symptoms when grown on Fe-deficient media compared to basal MGRL solid medium. iTRAQ-OFFGEL approach identified 909 differentially expressed proteins common to all three biological replicates; the majority were transporters or proteins involved in photosynthesis, and ribosomal proteins. Interestingly, protein expression changes between excess Zn and Fe deficiency showed similar pattern. Further, the changes due to excess Zn were dramatically restored by the addition of Fe. To obtain biological insight into Zn and Fe cross-talk, we focused on transporters, where STP4 and STP13 sugar transporters were predominantly expressed and responsive to Fe-deficient conditions. Plants grown on Fe-deficient conditions showed significantly increased level of sugars. These results suggest that Fe deficiency might lead to the disruption of sugar synthesis and utilization.

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supporting

confidence: 90%

Quantitative proteomics of Arabidopsis shoot microsomal proteins reveals a cross-talk between excess zinc and iron deficiency

et al. 2015

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“…In A. thaliana , it is well documented that Zn excess results in a secondary Fe deficiency response (Wang et al ., ; Shanmugam et al ., , ). Comparative studies of A. halleri ssp .…”

Section: Discussionmentioning

confidence: 99%

Adaptation to high zinc depends on distinct mechanisms in metallicolous populations of Arabidopsis halleri

Schvartzman

Corso²,

Fataftah

et al. 2018

New Phytologist

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Zinc (Zn) hyperaccumulation and hypertolerance are highly variable traits in Arabidopsis halleri. Metallicolous populations have evolved from nearby nonmetallicolous populations in multiple independent adaptation events. To determine whether these events resulted in similar or divergent adaptive strategies to high soil Zn concentrations, we compared two A. halleri metallicolous populations from distant genetic units in Europe (Poland (PL22) and Italy (I16)). The ionomic (Inductively Coupled Plasma Atomic Emission Spectroscopy (ICP-AES)) and transcriptomic (RNA sequencing (RNA-Seq)) responses to growth at 5 and 150 μM Zn were analyzed in root and shoot tissues to examine the contribution of the geographic origin and treatment to variation among populations. These analyses were enabled by the generation of a reference A. halleri transcriptome assembly. The genetic unit accounted for the largest variation in the gene expression profile, whereas the two populations had contrasting Zn accumulation phenotypes and shared little common response to the Zn treatment. The PL22 population displayed an iron deficiency response at high Zn in roots and shoots, which may account for higher Zn accumulation. By contrast, I16, originating from a highly Zn-contaminated soil, strongly responded to control conditions. Our data suggest that distinct mechanisms support adaptation to high Zn in soils among A. halleri metallicolous populations.

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“…The results presented for plants from populations B and W suggest an increase of the Fe accumulation in the shoots with an increasing accumulation of Zn and Cd in the shoots (Table II). A possible mechanism of the interaction between Zn and Fe, which prevents the toxic effect of Zn, was proposed by Shanmugam et al (2013). Recent reports also confirmed an increase in the accumulation of iron in the leaves of plants characterized by a hyperaccumulation of Zn and Cd (Stein et al, 2017).…”

Section: Parametersmentioning

confidence: 94%

Photosynthetic Efficiency as Bioindicator of Environmental Pressure in A. halleri

et al. 2017

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In earlier ecophysiological studies that were conducted on Arabidopsis halleri plants, scientists focused on the mechanisms of Cd and Zn hyperaccumulation but did not take into consideration the environmental factors that can significantly affect the physiological responses of plants in situ. In this study, we investigated A. halleri that was growing on two nonmetalliferous and three metalliferous sites, which were characterized by different environmental conditions. We compared these populations in order to find differences within the metallicolous and nonmetallicolous groups that have not yet been investigated. The concentrations of several elements in the plant and soil samples also were investigated. To our knowledge, the concentration and fluorescence of chlorophyll were measured for A. halleri in situ for the first time. Our study confirmed the hyperaccumulation of Cd and Zn for each metallicolous population. For the metallicolous populations, the inhibition of parameters that describe the efficiency of the photosynthetic apparatus with increasing accumulations of heavy metals in the shoots also was observed. It was found that the nonmetallicolous plant populations from the summit of Ciemniak Mountain had larger antenna dimensions and chlorophyll content but a lower percentage of active reaction centers. To our knowledge, in this study, the internal high physiological diversity within the populations that inhabit metalliferous and nonmetalliferous sites is presented for the first time.

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Control of Zn uptake in Arabidopsis halleri: a balance between Zn and Fe

Cited by 56 publications

References 58 publications

Quantitative proteomics of Arabidopsis shoot microsomal proteins reveals a cross-talk between excess zinc and iron deficiency

Quantitative proteomics of Arabidopsis shoot microsomal proteins reveals a cross-talk between excess zinc and iron deficiency

Adaptation to high zinc depends on distinct mechanisms in metallicolous populations of Arabidopsis halleri

Photosynthetic Efficiency as Bioindicator of Environmental Pressure in A. halleri

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