Control of weft yarn or density improves biocompatibility of PET small diameter artificial blood vessels

Abstract

Polyethylene glycol terephthalate (PET) fabrics with woven structures have proved to be quite effective for use on large diameter artificial blood vessels. However, their use within small-diameter artificial blood vessels has been associated with poor long-term patency, a problem resulting from slow endothelialization on PET and an over hyperplasia of smooth muscle cells. Previous research from our laboratory has revealed that ICAM-1 can be used as a marker to investigate cell adhesion, an effect which was closely associated with cell behavior on the surface of polycaprolactone (PCL) films. Moreover, we found that the coarseness or pore size of the surface exerts considerable influence on cell adhesion and proliferation on PCL films. In this study, we successfully fabricated six types of PET woven fabrics with varying gradients of tightness and porosities. Levels of ICAM-1 expression (membrane ICAM-1 & soluble ICAM-1) were then determined in these woven fabrics. Our results show that increased levels of mICAM-1 and decreased levels of sICAM-1 expression were obtained in HUVECs seeded on these six samples. These findings indicate that cell adhesion and proliferation on fabric surfaces were strongly influenced by their structural parameters, in particular the initial adhesion between the cell and fabric surface. In addition, we also found that extracellular matrix adhesion tends to prefer flat and tight surfaces, which promotes cell-cell and cell-matrix interactions, as well as the endothelialization on the surface of PET fabrics. These findings provide some novel insights with regard to the design and application of small-diameter artificial blood vessels. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 954-964, 2018.

An Efficient Surface Modification Strategy Improving Endothelialization with Polydopamine Nanoparticles and REDV Peptides for Stent-Grafts

“…With an increase in the culture time, gaps between fibers were filled with extracellular matrix (b in Figure ). It indicated that fibers provided an adhesion platform for ECs at the beginning . However, cells preferred to migrate to places with more nutrition like yarn gaps, which nutrition can permeate through.…”

“…It indicated that fibers provided an adhesion platform for ECs at the beginning. 29 However, cells preferred to migrate to places with more nutrition like yarn gaps, which nutrition can permeate through. For stent-grafts modified with PDA-NP, ECs tended to stick and grow on PDA-NP (c in Figure 8).…”

Use of an in vitro dynamic culture system to assess flow shear forces upon cell adhesion within different structures

“…The designed warp density was 850/10 cm and the weft density was 700/10 cm. Fabrics were washed three times (30 min each) with use of an ultrasonic cleaner and dried . Based upon previous results, fabrics were subjected to Ar 2 low temperature plasma treatment under varying conditions as summarized in Table .…”

“…Cells were treated with 4% paraformaldehyde for 2 h followed by 4',6‐diamidino‐2‐phenylindole (DAPI), and Tetramethylrhodamine (TRITC) was used to stain the cells . Confocal laser scanning microscopy was used to observe adhesion of cells to the material surface after being subjected to dynamic culture.…”

Synthesis of polyester by means of polycondensation of diol ester and dicarboxylic acid ester through ester–ester exchange reaction

“…Polyesters have been used for decades in a broad range of applications including films, fibers, and moldable engineering parts, and have recently received much attention as biodegradable and biocompatible polymers. [1][2][3][4][5][6][7][8][9] General synthetic methods for polyester include ring-opening polymerization of lactones and polycondensation of diol and dicarboxylic acid derivatives. In particular, the latter method can afford a wide variety of polyesters by changing the combination of the two monomers.…”