Control of water content of non‐metabolizing kidney slices by sodium chloride and polyethylene glycol (Peg 6000)

Robinson, J. R.

doi:10.1113/jphysiol.1971.sp009378

Cited by 47 publications

(18 citation statements)

References 8 publications

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“…Different from the others, Polysol contains polyethylene glycol (PEG) that enables both the required oncotic pressure and the relatively low viscosity (1.8 cP). PEG stabilizes lipid membranes and contributes to less membrane permeability [25,26] , thus preventing osmotic cell swelling and vascular endothelial damage [27] . Moreover, PEG also act as a OFR scavenger, markedly reducing lipid peroxidation upon preservation/ reperfusion process [27,28] .…”

Section: Discussionmentioning

confidence: 99%

Experimental small bowel preservation using Polysol: A new alternative to University of Wisconsin solution, Celsior and histidine-tryptophan-ketoglutarate solution?

Wei

Hata²,

Doorschodt³

et al. 2007

WJG

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AIM:To evaluate the potential of Polysol, a newly developed preservation solution, in cold storage of small bowel grafts, compared with the current standards, University of Wisconsin solution (UW), Celsior and histidine-tryptophan-ketoglutarate solution (HTK). METHODS:Male Wistar rats were used as donors. Small bowels were retrieved, flushed and then stored in the respective 4 solutions for 18 h at 4℃. Functional integrity of the grafts was evaluated by isolated reperfusion with oxygenated Krebs-Henseleit buffer at 37℃ for 30 min in all 4 groups. RESULTS:Polysol preservation exhibited the highest tissue ATP concentration and the lowest release of LDH. Malondialdehyde, an index for tissue lipid peroxidation, w a s a l s o t h e l o w e s t i n Po l y s o l . T i s s u e o x y g e n consumption was significantly higher in Polysol than in the others. Of interest, UW-storage promoted 10-fold higher apoptosis than in the others. Moreover, electron microscopy revealed that the mucosal villi/micro-villi formation and the cell organelles, including mitochondria, were both significantly better preserved in Polysol, while deleterious alterations were apparent in the others, most notably in UW. Although Celsior and HTK exhibited the better trend of results than UW in some parameters, but could not reach the over-all superiority to UW. CONCLUSION:Cold storage using Polysol resulted in significantly better integrity and function of small bowel grafts than UW. Hence, Polysol may be a novel alternative for the small bowel preservation.

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Section: Discussionmentioning

confidence: 99%

Experimental small bowel preservation using Polysol: A new alternative to University of Wisconsin solution, Celsior and histidine-tryptophan-ketoglutarate solution?

Wei

Hata²,

Doorschodt³

et al. 2007

WJG

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“…wt. 6000 (PEG 6000) has been used to prevent tissue swelling in non-metabolizing tissue (Davey & Skegg, 1971;Little & Robinson, 1967;McIver & Raine, 1972;Robinson, 1971;Wiggins, 1964Wiggins, , 1965Wiggins, , 1967. Interpretation of these experiments has depended on the assumption that PEG 6000 equilibrates in the extracellular fluid but does not enter cells, its osmotic effect thereby balancing the colloid osmotic pressure of the intracellular macromolecules.…”

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confidence: 99%

Extracellular space in some isolated tissues

McIver¹,

Macknight²

1974

The Journal of Physiology

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1. The spaces occupied by isotopically labelled inulin, polyethylene glycol, mol. wt. 4000 (PEG 4000), polyethylene glycol, mol. wt. 1000 (PEG 1000) and sucrose in metabolizing mammalian kidney and liver slices and in toad bladder epithelial cell preparations incubated in vitro have been examined. 2. In slices of mammalian tissue, and in homogenized liver, it proved impossible to extract inulin completely from tissue which had been dried. However, inulin was recovered as completely from both dried and undried toad bladder epithelial cells scraped from hemibladders incubated in vitro. 3. PEG 4000 occupied a space in all preparations similar to that from which inulin was extracted in dried tissue. 4. PEG 1000 and sucrose entered cellular water in mammalian slices, but PEG 1000 occupied a similar space to inulin in toad bladder epithelial cell preparations. 6. It is concluded that inulin enters cellular water in mammalian slices from which after drying of the slices it cannot be extracted. It thus rather fortuitously provides a measure of extracellular water under these conditions. In preparations of toad bladder epithelial cells inulin seems to be a satisfactory extracellular marker. PEG 4000, which did not appear to enter cellular water also allows a reasonable estimate of extracellular water. PEG 1000 is a suitable extracellular marker for toad bladder epithelial cell preparations but not for mammalian slices. Sucrose entered cellular water in both slices and toad bladder epithelial cells and is not a satisfactory extracellular marker in these tissues.

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“…This effect of electrolytes on non-metabolizing cells is dependent on the ionic strength of the medium. Robinson (1971) has demonstrated an inverse relation between medium NaCl concentration and the amount of PEG necessary to prevent swelling in non-metabolizing slices. Thus, it is clear that in the absence of metabolically dependent volume regulation at least, the electrolyte concentration of the medium can influence cellular volume.…”

Section: Discussionmentioning

confidence: 99%

The influence of electrolytes on the volume of non‐metabolizing renal cortical cells

McIver¹,

Raine²

1972

The Journal of Physiology

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SUMBMARY1. The metabolism ofrat renal cortex slices was inhibited by iodoacetate and anoxia, and swelling was prevented by the presence in the medium of 7-2 g polyethylene glycol 6000/100 ml. (referred to as PEG medium).2. Slices were incubated for up to 12 hr in PEG medium, and in PEG media containing 440 m-osmole/kg H20 of an electrolyte (LiCl, NaCl, KCl or RbQl), or a non-electrolyte (glucose).3. It was concluded that the slices in all media were at equilibrium with the medium after incubation for 8 hr.4. Slices in the medium containing glucose reached the same equilibrium water content as those in the PEG medium, but slices in all the electrolyte media had significantly lower equilibrium water contents, although these did not differ significantly from each other.5. It is suggested that the results demonstrate a non-specific effect of electrolytes on the swelling of non-metabolizing cells. DITRODUCTIONDespite the fact that their colloids depress the activity of the intracellular fluids, metabolizing cells maintain stable volumes. Leaf (1956, 1959) accounted for this stability in terms of a 'double Donnan' system, in which Na, actively held in an obligatorily extracellular position, balanced the osmotic pressure of the cell colloids, and prevented swelling.Many workers have shown that swelling does occur when the metabolism of isolated tissues is interfered with, thereby inactivating the mechanisms normally responsible for Na exclusion. However, Opie (1949), Robinson (1950) and others found that this swelling was prevented by immersion of the tissue in highly concentrated solutions of NaCl. Deyrup. (1953) suggested that these observations could be explained on the basis of a greater permeability of the cell membrane to water than to solutes; if

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Control of water content of non‐metabolizing kidney slices by sodium chloride and polyethylene glycol (Peg 6000)

Cited by 47 publications

References 8 publications

Experimental small bowel preservation using Polysol: A new alternative to University of Wisconsin solution, Celsior and histidine-tryptophan-ketoglutarate solution?

Experimental small bowel preservation using Polysol: A new alternative to University of Wisconsin solution, Celsior and histidine-tryptophan-ketoglutarate solution?

Extracellular space in some isolated tissues

The influence of electrolytes on the volume of non‐metabolizing renal cortical cells

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