Control of Vegetative to Reproductive Phase Transition Improves Biomass Yield and Simultaneously Reduces Lignin Content in Medicago truncatula

Tadege, Million; Chen, Fang; Murray, Jeremy D.; Wen, Jiangqi; Ratet, Pascal; Udvardi, Michael K.; Dixon, Richard A.; Mysore, Kirankumar S.

doi:10.1007/s12155-014-9565-y

Cited by 23 publications

(26 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In different plant species, the flowering time has often been linked to lignification when searching for a marker of the biomass yield. In Medicago truncatula , a delayed flowering mutant had higher biomass and lower lignin amounts compared to the wild type at the same age [ 51 ]. Nevertheless, the correlation between flowering time and lignification may dependent on the clade.…”

Section: Discussionmentioning

confidence: 99%

UDP-GLYCOSYLTRANSFERASE 72E3 Plays a Role in Lignification of Secondary Cell Walls in Arabidopsis

Baldacci‐Cresp

Roy

Huss

et al. 2020

IJMS

View full text Add to dashboard Cite

Lignin is present in plant secondary cell walls and is among the most abundant biological polymers on Earth. In this work we investigated the potential role of the UGT72E gene family in regulating lignification in Arabidopsis. Chemical determination of floral stem lignin contents in ugt72e1, ugt72e2, and ugt72e3 mutants revealed no significant differences compared to WT plants. In contrast, the use of a novel safranin O ratiometric imaging technique indicated a significant increase in the cell wall lignin content of both interfascicular fibers and xylem from young regions of ugt72e3 mutant floral stems. These results were globally confirmed in interfascicular fibers by Raman microspectroscopy. Subsequent investigation using a bioorthogonal triple labelling strategy suggested that the augmentation in lignification was associated with an increased capacity of mutant cell walls to incorporate H-, G-, and S-monolignol reporters. Expression analysis showed that this increase was associated with an up-regulation of LAC17 and PRX71, which play a key role in lignin polymerization. Altogether, these results suggest that UGT72E3 can influence the kinetics of lignin deposition by regulating monolignol flow to the cell wall as well as the potential of this compartment to incorporate monomers into the growing lignin polymer.

show abstract

Section: Discussionmentioning

confidence: 99%

UDP-GLYCOSYLTRANSFERASE 72E3 Plays a Role in Lignification of Secondary Cell Walls in Arabidopsis

Baldacci‐Cresp

Roy

Huss

et al. 2020

IJMS

View full text Add to dashboard Cite

show abstract

“…If Alamo has a mutation in one or more of the photoperiod sensitivity genes, re‐introducing those would make Alamo produce more biomass in the LD growth conditions appropriate for temperate regions including the United States. In any case, the underlying hypothesis is that when flowering is delayed either by knocking out major floral activators or by expressing major floral repressors, more vegetative shoot is accumulated above ground with potentially reduced lignin content, improving total biomass yield and saccharification efficiency (Tadege et al ). In support of this, delaying flowering in switchgrass by expressing Corngrass 1 microRNA, as well as PvmiR156 was reported to improve feedstock yield and digestibility (Chuck et al ; Fu et al ).…”

Section: Discussionmentioning

confidence: 99%

“…Flowering time is critical to accumulation of biomass because delaying flowering time is associated with increased vegetative biomass yield in several species including Miscanthus, sorghum and switchgrass (Casler 2014;Chuck et al 2011;Dong et al 2015;Fu et al 2012;Jensen et al 2011;Mullet et al 2014;Olson et al 2012;Rooney et al 2007;Sanderson et al 1999;Talbert et al 1983). In Medicago truncatula, delayed flowering improves both biomass yield and biomass digestibility (Tadege et al 2015). In switchgrass, lowland and upland cultivars, for example Alamo and Summer, respectively, vary greatly in their maturity and biomass yield; the lowland genotypes flower significantly later than the upland genotypes and produce more biomass (Alexopoulou et al 2008;Casler 2014;Casler et al 2007;Stroup et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Control of floral transition in the bioenergy crop switchgrass

Niu

Lin

et al. 2016

Plant Cell & Environment

Self Cite

View full text Add to dashboard Cite

Switchgrass (Panicum virgatum L.), a perennial warm season bunchgrass native to North America, has been a target in the U.S. as a renewable bioenergy crop because of its ability to produce moderate to high biomass yield on marginal soils. Delaying flowering can increase vegetative biomass production by allowing prolonged growth before switching to the reproductive phase. Despite the identification of flowering time as a biomass trait in switchgrass, the molecular regulatory factors involved in controlling floral transition are poorly understood. Here we identified PvFT1, PvAPL1-3 and PvSL1, 2 as key flowering regulators required from floral transition initiation to development of floral organs. PvFT1 expression in leaves is developmentally regulated peaking at the time of floral transition, and diurnally regulated with peak at approximately 2 h into the dark period. Ectopic expression of PvFT1 in Arabidopsis, Brachypodium and switchgrass led to extremely early flowering, and activation of FT downstream target genes, confirming that it is a strong activator of flowering in switchgrass. Ectopic expression of PvAPL1-3 and PvSL1, 2 in Arabidopsis also activated early flowering with distinct floral organ phenotypes. Our results suggest that switchgrass has conserved flowering pathway regulators similar to Arabidopsis and rice.

show abstract

“…The timing of flowering is a critical agronomic trait in plants because of its major effects on reproductive and vegetative productivity ( Julier et al , 2007 ; Jung and Müller, 2009 ; Yeoh et al , 2011 ; Tadege et al , 2015 ). Plants have evolved sophisticated flowering gene networks that integrate different internal and external signals before making the decision to flower ( Putterill et al , 2004 ; Srikanth and Schmid, 2011 ).…”

Section: Introductionmentioning

confidence: 99%

A SOC1-like gene MtSOC1a promotes flowering and primary stem elongation in Medicago

Jaudal

Zhang

Che

et al. 2018

Journal of Experimental Botany

Self Cite

View full text Add to dashboard Cite

show abstract

Control of Vegetative to Reproductive Phase Transition Improves Biomass Yield and Simultaneously Reduces Lignin Content in Medicago truncatula

Cited by 23 publications

References 72 publications

UDP-GLYCOSYLTRANSFERASE 72E3 Plays a Role in Lignification of Secondary Cell Walls in Arabidopsis

UDP-GLYCOSYLTRANSFERASE 72E3 Plays a Role in Lignification of Secondary Cell Walls in Arabidopsis

Control of floral transition in the bioenergy crop switchgrass

A SOC1-like gene MtSOC1a promotes flowering and primary stem elongation in Medicago

Contact Info

Product

Resources

About