Control of tissue dimensions in the developing neural tube and somites

Minchington, Thomas G; Rus, Stefanie; Kicheva, Anna

doi:10.1016/j.coisb.2023.100459

Cited by 4 publications

References 72 publications

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Self-organised pattern formation in the developing neural tube by a temporal relay of BMP signalling

Lehr,

Brückner,

Greunz-Schindler

et al. 2023

Preprint

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Developing tissues interpret dynamic changes in morphogen activity to generate cell type diversity. To quantitatively study BMP signalling dynamics in the vertebrate neural tube, we developed a new ES cell differentiation system tailored for growing tissues. Differentiating cells form striking self-organised patterns of dorsal neural tube cell types driven by sequential phases of BMP signalling that are observed both in vitro and in vivo. Data-driven biophysical modelling showed that these dynamics result from coupling fast negative feedback with slow positive regulation of signalling by the specification of an endogenous BMP source. Thus, in contrast to relays that propagate morphogen signalling in space, we uncover a BMP signalling relay that operates in time. This mechanism allows rapid initial concentrationsensitive response that is robustly terminated, thereby regulating balanced sequential cell type generation. Altogether, our study provides an experimental and theoretical framework to understand how signalling dynamics are exploited in developing tissues.

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Self-organised pattern formation in the developing neural tube by a temporal relay of BMP signalling

Lehr,

Brückner,

Greunz-Schindler

et al. 2023

Preprint

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Differential proliferation regulates multi-tissue morphogenesis during embryonic axial extension: Integrating viscous modeling and experimental approaches

Romanos,

Salisbury,

Stephan

et al. 2024

Preprint

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The study of how mechanical interactions and different cellular behaviors affect tissues and embryo shaping has been and remains an important challenge in biology. Axial extension is a morphogenetic process that results in the acquisition of the elongated shape of the vertebrate embryonic body. Several adjacent tissues are involved in the process, including the tissues that form the spinal cord and musculoskeletal system: the neural tube and the paraxial mesoderm, respectively. Although we have a growing understanding of how each of these tissues elongates, we still need to fully understand the morphogenetic consequences of their growth and mechanical interactions. In this study, we develop a 2D multi-tissue continuum-based mathematical model to simulate and study how differential growth, tissue biophysical properties, and mechanical interactions affect the morphogenesis of the embryonic body during axial extension. Our model captures the long-term dynamics of embryonic posterior tissues previously observed in vivo by time-lapse imaging of bird embryos. It reveals the underestimated influence of differential tissue proliferation rates in inter-tissue interaction and shaping by capturing the relative impact of this process on tissue dynamics. We verified the predictions of our model in quail embryos by showing that decreasing the rate of cell proliferation in the paraxial mesoderm affects long-term tissue dynamics and shaping of both the paraxial mesoderm and the neighboring neural tube. Overall, our work provides a new theoretical platform to consider the long-term consequences of tissue differential growth and mechanical interactions on morphogenesis.

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Fabrication of elastomeric stencils for patterned stem cell differentiation

Lehr,

Merrin,

Kulig

et al. 2024

Preprint

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Stem cell differentiation with controlled geometry results in reproducible pattern formation. In contrast to constraining differentiating cells on micropatterned surfaces, we initialise colony formation using elastomeric stencils that adhere to culture dishes and create microwells with defined sizes and shapes. After colony formation, stencils are removed to allow colony growth and cell migration. Stencil fabrication involves mould production by photolithography followed by replica-moulding polydimethylsiloxane (PDMS). This approach produces reproducible two-dimensional organoids tailored for quantitative studies of growth control and pattern formation.

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Control of tissue dimensions in the developing neural tube and somites

Cited by 4 publications

References 72 publications

Self-organised pattern formation in the developing neural tube by a temporal relay of BMP signalling

Self-organised pattern formation in the developing neural tube by a temporal relay of BMP signalling

Differential proliferation regulates multi-tissue morphogenesis during embryonic axial extension: Integrating viscous modeling and experimental approaches

Fabrication of elastomeric stencils for patterned stem cell differentiation

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