Control of ticks resistant to immunization with Bm86 in cattle vaccinated with the recombinant antigen Bm95 isolated from the cattle tick, Boophilus microplus

García-García, Jose Carlos; Montero, Carlos; Redondo, María C.; Vargas, Marlene; Canales, Mario; Boué, Oscar; Rodrı́guez, Manuel; Joglar, Marisdania; Machado, Humberto; González, Ileana L.; Valdés, Mario; Méndez, Luis; Fuente, José de la

doi:10.1016/s0264-410x(99)00548-4

Cited by 173 publications

(118 citation statements)

References 24 publications

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“…(B.) microplus from Argentina (GARCÍA-GARCÍA et al, 2000). A link between vaccine efficacy and sequence variability has been questioned because there are factors other than primary amino acid sequence of the antigen that influence the efficacy of a vaccine (WILLADSEN, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Vaccines provide an alternative tool for tick control (WILLADSEN; JONGEJAN, 1999). Integrated tick control programs that include vaccines can reduce the use of chemical acaricides, lower the cost of animal production, mitigate environmental contamination impacts, and decrease the risk of chemical residues in animal-derived products (DE LA FUENTE et al, 1998;GARCÍA-GARCÍA et al, 2000;REDONDO et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

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Bovine immunoprotection against Rhipicephalus (Boophilus) microplus with recombinant Bm86-Campo Grande antigen

Cunha

León

Pinto

et al. 2012

Rev. Bras. Parasitol. Vet.

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The southern cattle fever tick, Rhipicephalus (Boophilus) microplus, is no doubt the most economically important ectoparasite of cattle globally. The inappropriate use of chemical acaricides has driven the evolution of resistance in populations of R. (B.) microplus. Anti-tick vaccines represent a technology that can be combined with acaricides in integrated control programs to mitigate the impact of R. (B.) microplus. The recombinant form of Bm86 antigen from the Campo Grande (rBm86-CG) strain of R. (B.) microplus was produced using the Pichia pastoris expression system to test its ability to immunoprotect cattle against tick infestation. Secretion of rBm86-CG by P. pastoris through the bioprocess reported here simplified purification of the antigen. A specific humoral immune response was detected by ELISA in vaccinated cattle. Immunoblot results revealed that polyclonal antibodies from vaccinated cattle recognized a protein in larval extracts with a molecular weight corresponding to Bm86. The rBm86-CG antigen showed 31% efficacy against the Campo Grande strain of R. (B.) microplus infesting vaccinated cattle. The rBm86-CG is an antigen that could be used in a polyvalent vaccine as part of an integrated program for the control of R. (B.) microplus in the region that includes Mato Grosso do Sul.Keywords: Tick, Rhipicephalus (Boophilus) microplus, anti-tick vaccine, recombinant Bm86, Pichia pastoris. ResumoO carrapato Rhipicephalus (Boophilus) microplus é, sem dúvidas, o ectoparasito economicamente mais importante para o gado a nível mundial. A utilização inadequada de acaricidas tem impulsionado a evolução da resistência em populações de R. (B.) microplus. Vacinas contra o carrapato representam uma tecnologia que pode ser combinada com acaricidas em programas de controle integrado para diminuir o impacto de R. (B.) microplus. A forma recombinante da Bm86 da cepa Campo Grande (rBm86-CG) de R. (B.) microplus foi produzido utilizando o sistema de expressão em Pichia pastoris para testar sua capacidade de imunoproteção ao gado contra a infestação de carrapatos. A secreção de rBm86-CG em P. pastoris pelo bioprocesso, simplificou a purificação do antígeno. A resposta imune humoral específica foi detectada por ELISA em soros de bovinos vacinados. Resultados de "imunoblot" revelaram que anticorpos policlonais de bovinos vacinados reconheceram uma proteína em extratos de larvas com um peso molecular correspondente à Bm86. O antígeno rBm86-CG mostrou eficácia de 31% contra a amostra CG de R. (B.) microplus utilizada para infestar os bovinos vacinados. Pelos resultados obtidos, concluímos que a rBm86-CG é um antígeno que pode ser usado em uma vacina polivalente, como parte de um programa integrado para o controle de R. (B.) microplus no estado do Mato Grosso do Sul, Brasil.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bovine immunoprotection against Rhipicephalus (Boophilus) microplus with recombinant Bm86-Campo Grande antigen

Cunha

León

Pinto

et al. 2012

Rev. Bras. Parasitol. Vet.

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“…microplus strain A, in a pen trial under production conditions, decreasing ticks numbers on vaccinated animals and, therefore, reducing the frequency of acaricide treatments needed (GARCIA-GARCIA et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

“…Limited success is achieved when acaricides are used as the main control method, and major drawbacks are associated with development of resistance by ticks and chemical residue toxicity in the environment and animal products (GRISI et al, 2002). The use of vaccines for tick control in association with chemicals and pasture rotation may open possibilities for integrated control, reducing the problems caused by development of resistance by the parasites and environmental and food product contamination (GARCIA-GARCIA et al, 2000).An effective vaccine was developed based on the Rhipicephalus (Boophilus) microplus gut Bm86 antigen (KEMP et al, 1989; DE LA FUENTE et al, 1995 , respectively. The PCR product was cloned into pMOSBlue and sequenced.…”

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confidence: 99%

Comparison of predicted binders in Rhipicephalus (Boophilus) microplus intestine protein variants BM86 Campo Grande strain, BM86 and BM95

Andreotti

Pedroso

Caetano

et al. 2008

Rev. Bras. Parasitol. Vet.

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Rev. Bras. Parasitol. Vet., 17, 1, 93-98 (2008) (Brazil. J. Vet. Parasitol.) RESUMOO objetivo deste estudo foi analisar a seqüência de Bm86 cepa Campo Grande comparando-a com os antígenos Bm86 e Bm95 das preparações TickGard PLUS e Gavac TM , respectivamente. O produto de PCR foi clonado em PMOSBlue e seqüenciado. Para calcular os conteúdos de alfa-hélice e fita beta do polipeptídio previsto, foi utilizada a ferramenta de prognóstico de estrutura secundária PSIPRED. O perfil de hidrofobicidade foi calculado usando os algoritmos de Hopp e Woods, além da identificação das possíveis regiões de ligação com MHC classe I nos antígenos. O alinhamento "pair-wise" revelou que a similaridade entre Bm86 cepa Campo Grande e Bm86 é 0,2% maior que aquela entre Bm86 cepa Campo Grande e Bm95. As identidades foram de 96,5% e 96,3%, respectivamente. Com relação à hidrofobicidade, os resultados sugerem que a maior diferença entre as seqüências está localizada em duas regiões específicas. PALAVRAS-CHAVE:Rhipicephalus (Boophilus) microplus, carrapato, Bm86, Bm95, antígeno. INTRODUCTIONTick control remains a serious problem for cattle farms in Brazil. Limited success is achieved when acaricides are used as the main control method, and major drawbacks are associated with development of resistance by ticks and chemical residue toxicity in the environment and animal products (GRISI et al., 2002). The use of vaccines for tick control in association with chemicals and pasture rotation may open possibilities for integrated control, reducing the problems caused by development of resistance by the parasites and environmental and food product contamination (GARCIA-GARCIA et al., 2000).An effective vaccine was developed based on the Rhipicephalus (Boophilus) microplus gut Bm86 antigen (KEMP et al., 1989; DE LA FUENTE et al., 1995 , respectively. The PCR product was cloned into pMOSBlue and sequenced. The secondary structure prediction tool PSIPRED was used to calculate alpha helices and beta strand contents of the predicted polypeptide. The hydrophobicity profile was calculated using the algorithms from the Hopp and Woods method, in addition to identification of potential MHC class-I binding regions in the antigens. Pair-wise alignment revealed that the similarity between Bm86 Campo Grande strain and Bm86 is 0.2% higher than that between Bm86 Campo Grande strain and Bm95 antigens. The identities were 96.5% and 96.3% respectively. Major suggestive differences in hydrophobicity were predicted among the sequences in two specific regions.

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“…It has been documented that habitat adaptation could have resulted in strains with variations that have in fact altered the susceptibility of different strains of ticks to Bm86 based vaccines (Cobon et al 1996;Garcia-Garcia et al 1999, 2000. Twenty-one amino acid substitutions were observed between the Yeerongpilly (Australian) Bm86 and Bm85 from an Argentinean isolate of B. microplus (strain A), expressed in Pichia pastoris, resulted in a new Bm86 homologue, Bm95 gene (Garcia-Garcia et al 2000).…”

Section: Discussionmentioning

confidence: 99%

Partial sequencing of Bm86 gene for studying the phylogeny of an Indian isolate of Rhipicephalus (Boophilus) microplus tick

Anbarasi¹,

Latha

Raj

et al. 2013

J Parasit Dis

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Tick gut glycoprotein, designated as Bm86, found on the luminal surface of the plasma membrane of gut epithelial cells of Boophilus microplus, which is a concealed antigen, has been used as vaccine candidate molecule for immunization against ticks. To better understand the molecular diversity of Bm86 gene in ticks, a portion of the cDNA was sequenced from an Indian isolate of B. microplus. Comparison of nucleotide sequence revealed that Indian isolate had 97 % homology (18 polymorphisms) with that of the Australian isolate and 96 % homology (20 polymorphisms) with that of the Cuban vaccine strain. Further, the Indian isolate differed from the Cuban vaccine isolate at 7 amino acid loci, including 5 substitutions (at residues 88, 94, 175, 176 and 177) and 2 deletions (at 183 and 184). However, protein prediction studies did not show any difference in the putative antigenic epitopes of the protein expressed.

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Control of ticks resistant to immunization with Bm86 in cattle vaccinated with the recombinant antigen Bm95 isolated from the cattle tick, Boophilus microplus

Cited by 173 publications

References 24 publications

Bovine immunoprotection against Rhipicephalus (Boophilus) microplus with recombinant Bm86-Campo Grande antigen

Bovine immunoprotection against Rhipicephalus (Boophilus) microplus with recombinant Bm86-Campo Grande antigen

Comparison of predicted binders in Rhipicephalus (Boophilus) microplus intestine protein variants BM86 Campo Grande strain, BM86 and BM95

Partial sequencing of Bm86 gene for studying the phylogeny of an Indian isolate of Rhipicephalus (Boophilus) microplus tick

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