Control of the developmental pathway of tobacco pollen in vitro

Abstract

We developed a new method for culture of isolated pollen. Using highly homogeneous populations of immature pollen grains of Nicotiana tabacum L. prepared by means of Percoll density gradient centrifugation, we could direct their developmental pathway by regulating certain culture conditions. When the pollen population was cultured in basal medium with glutamine, most pollen grains underwent normal maturation. On the other hand, when first cultured in basal medium without glutamine, most pollen grains did not mature but after transfer to medium with glutamine and sucrose began to divide. This method for inducing pollen cell division was possible only with midbinucleate pollen grains which are characterized by having no central vacuole and no or only a few starch grains. Evidently, some essential changes necessary for the embryogenic response can be induced by glutamine starvation only in pollen grains at a specific stage.

Refining the method for eggplant microspore culture: effect of abscisic acid, epibrassinolide, polyethylene glycol, naphthaleneacetic acid, 6-benzylaminopurine and arabinogalactan proteins

“…This is in accordance with previous results in tobacco or 1 barley. In tobacco, Kyo and Harada (1986) on embryogenesis induction. However, our experiments with βGlcY indicated that at 6 least in our system, the principal responsible of the increases in callus number, size and 7 weight was the ~10% of AGPs present in gum arabic, and not the major fraction of 8…”

Expression of the [beta]-Glucuronidase Gene in Pollen of Lily (Lilium longiflorum), Tobacco (Nicotiana tabacum), Nicotiana rustica, and Peony (Paeonia lactiflora) by Particle Bombardment

“…Pollen of tobacco and N. rustica was suspended in medium C (Kyo and Harada, 1986) at a density of 5 X 104 cells/mL. Pollen of P. lactiflora was suspended in M4 medium (Ono and Harashima, 1981) at a density of 105 cells/ mL.…”

Electron microscope studies on cold pretreated and non-pretreated anthers of maize (Zea mays L.) genotypes with different embryogenic capacities

“…Nevertheless, it is possible that microspores deprived of a nutrient supply from the mother plant may satisfy their sugar needs from the starch in the pollen wall tissues. Consequently, sugar starvation, which is considered to be a trigger of in vitro androgenesis (Wei et al, 1986;Kyo and Harada, 1986), probably does not occur in the microspores.…”