Control of the cell cycle in cultured plant cells

Gould, Alan R.; King, Patrick J.

doi:10.1080/07352688409382183

Cited by 82 publications

(42 citation statements)

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“…Suspension cultures provide a source of material whose environment can be readily manipulated to study the effects of hormonal and nutritional signals on gene expression and the cell cycle (King, 1977;Gould et al, 1981;Gould, 1984;Bayliss, 1985;Nagata et al, 1992). We used suspension cultures derived from callus tissue growing out from seed germinated in liquid media (see HU block-release of suspension-cultured cells was as described in Methods.…”

Section: Induction By Carbon Source and Phytohormonesmentioning

confidence: 99%

A family of cyclin D homologs from plants differentially controlled by growth regulators and containing the conserved retinoblastoma protein interaction motif.

et al. 1995

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Section: Induction By Carbon Source and Phytohormonesmentioning

confidence: 99%

A family of cyclin D homologs from plants differentially controlled by growth regulators and containing the conserved retinoblastoma protein interaction motif.

et al. 1995

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“…Alternative models such as transition probability (Smith & Martin, 1973;Shields, 1977) and sizer control (Fantes, 1977;Fantes & Nurse, 1977;Wheals, 1987) have been discussed and tested in relation to plant meristems (e.g. Gould, 1984;Armstrong & Francis, 1985;. Those arguments will not be re-iterated here because despite flirtations with various models the term cell cycle is here to stay.…”

Section: Introductionmentioning

confidence: 99%

The cell cycle in plant development

Francis

1992

New Phytologist

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summary The aim of this review is to discuss the molecular controls of the cell cycle in relation to higher plant development. An analysis is made of the current models of the cell cycle based on the biochemistry and genetics of the budding yeast, Saccharomyces cerevisiae and the fission yeast, Schizosaccharomyces pombe. What emerges are universal mechanisms observed in a wide range of taxonomic groups involving a group of protein kinases which regulate the transition from both post‐synthetic interphase (G2) to mitosis and from pre‐synthetic interphase (G1) to DNA synthetic‐(S) phase. The data are consistent in showing the activity of protein kinase complexes operating in conjunction with at least one dephosphorylating enzyme. The natural substrate(s) for the key cell division cycle gene product, p34cdc 2, has yet to be resolved although the nuclear lamins and microtubular apparatus are strong candidates. These models serve as a basis for assessing the cell cycle in higher plants. Mitosis and various stages of nuclear DNA replication are considered in relation to the presumed initiation and termination factors that regulate these events. In order to make a link between the cell cycle and plant development special consideration has been given to plant meristems. In particular, the activity of the cell cycle in cells that have the capacity to regenerate whole tissue systems (‘stem cells’) within the meristem is discussed. In the root meristem, the quiescent centre cells conform to a stem cell population; a non‐cycling stem cell may be immune to the morphogenetic signals that cause cycling cells to arrest and differentiate. The pericycle may act as a vestigial stem cell population. The shoot apex is also discussed in relation to both vegetative and floral growth. Although gradients of cell division exist in shoot meristems it is far less obvious where ‘stem’, or founder, cells reside in the apex. The way in which the cell cycle shortens on transition to floral growth is considered critical for identifying when the meristem becomes florally determined. Temperature and toxic metals are given special attention where it is emphasized that G1 phase becomes protracted when plants are stressed. Species that can tolerate stressful environments may have meristems in which a greater number of cells are competent for division. Finally, the cell cycle in vitro is discussed in relation to rapid changes in gene expression which are linked to the transition from G1 to S phase. The latter emerges as a key cell cycle transition for plant meristems both in vivo and in vitro.

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“…Immunohistochemical analysis revealed that PCNA is localized mainly in meristematic cells of soybean root tips, and this result suggests that the gene for a plant homologue of PCNA is also expressed preferentially in proliferating plant cells. To elucidate whether or not plant PCNA is involved in the replication of DNA, it is necessary to investigate the expression of plant PCNA during the cell cycle.In higher plants, a few synchronous culture systems were established (reviewed by Gould, 1984). Amino et al (1983) have established the synchronous cell division system in suspension cultures of Catharanthus roseus (periwinkle) cells by the double phosphate starvation method.…”

mentioning

confidence: 99%

“…In higher plants, a few synchronous culture systems were established (reviewed by Gould, 1984). Amino et al (1983) have established the synchronous cell division system in suspension cultures of Catharanthus roseus (periwinkle) cells by the double phosphate starvation method.…”

mentioning

confidence: 99%

Molecular cloning of the gene for plant proliferating‐cell nuclear antigen and expression of this gene during the cell cycle in synchronized cultures of Catharanthus roseus cells

Kodama

Ito

Ohnishi

et al. 1991

European Journal of Biochemistry

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A cDNA library was screened for plant proliferating-cell nuclear antigen (PCNA) from Catharanthus roseus (periwinkle). A 1 gtll cDNA library was constructed using poly(A)-rich RNA isolated from the cells in the S phase. A cDNA clone for PCNA was isolated by using a rice genomic clone, pCJ-1, which contains PCNA-related gene sequences. The cDNA contains an open reading frame of 804 nucleotides, encoding a protein of 268 amino acids with a molecular mass of 29765 Da. When conservative substitutions were included, a high degree of similarity (about 85%) was observed between the predicted amino acid sequence of periwinkle PCNA and that of human PCNA. Expression of mRNA for periwinkle PCNA was undetectable or very weak in quiescent cells, such as phosphate-starved cells, auxin-starved cells and cells in the stationary phase. In the synchronous progression of the cell cycle induced by the addition of phosphate or auxin, the active accumulation of periwinkle PCNA mRNA was observed preferentially in the S phase. When an inhibitor of DNA synthesis, aphidicolin, was added to the cells at the G I phase, an increase in the level of PCNA mRNA was observed. The partial inhibition of protein synthesis at the G1 phase by a protein inhibitor, anisomycin, caused the arrest of cells in the G1 phase. No increase of the level of periwinkle PCNA mRNA was observed in cells arrested at the GI phase by the inhibition of protein synthesis. These results indicate that the induction of mRNA for periwinkle PCNA occurred independently of the initiation of DNA replication, but that synthesis of certain proteins at the G1 phase was required for the induction of periwinkle PCNA mRNA at the S phase.Proliferating-cell nuclear antigen (PCNA) was initially recognized as a nuclear autoantigen which reacts with autoimmune sera from a certain group of patients with systemic lupus erythematosus (Miyachi et al., 1978). This antigen is an acidic, nuclear, non-histone protein and is present preferentially in the nuclei of proliferating mammalian cells and not in those of quiescent cells (Takasaki et al., 1984). Bravo et al. (1981) identified another nuclear protein, 'cyclin', with a molecular mass of about 36 kDa, whose synthesis is also correlated with the proliferative state of mammalian cells. Mathews et al. (1984) showed that PCNA and cyclin are identical. Immunofluorescence studies of the distribution of PCNA during the cell cycle have revealed that the expression of PCNA increases during the late G1 and early S phase, and also that the nuclear localization of PCNA changes dramatically in cells in the S phase Celis and Celis, 1985;Bravo, 1986;Kurki et al., 1986;Sadaie and Mathews, 1986;Moore et al., 1987). When the site Science, Tohoku University, Sendai, Japan 980 proliferating-cell nuclear antigen ; SV40, simian virus 40 of DNA synthesis was identified by labeling with bromodeoxyuridine or [3H]thymidine, a remarkable correlation was found between the distribution of PCNA and patterns of DNA synthesis . These observations suggest a role for PCNA ...

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Control of the cell cycle in cultured plant cells

Cited by 82 publications

References 87 publications

A family of cyclin D homologs from plants differentially controlled by growth regulators and containing the conserved retinoblastoma protein interaction motif.

A family of cyclin D homologs from plants differentially controlled by growth regulators and containing the conserved retinoblastoma protein interaction motif.

The cell cycle in plant development

Molecular cloning of the gene for plant proliferating‐cell nuclear antigen and expression of this gene during the cell cycle in synchronized cultures of Catharanthus roseus cells

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