Control of Surface Properties of Hyaluronan/Chitosan Multilayered Coatings for Tumor Cell Capture

Lima, Giulia Grassa; Neto, João Batista Maia Rocha; Carvalho, Hernandes F.; Beppu, Marisa Masumi

doi:10.3390/polysaccharides2020025

Cited by 4 publications

(3 citation statements)

References 71 publications

(84 reference statements)

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“…The INS is thus closely bound to the carrier and does not leak easily. (2) At a low pH, the carboxyl groups of HA were protonated [ 57 ]. An increasing number of carboxyl groups form intermolecular hydrogen bonds, which can lead to tight contraction of the polymer chain segments and highly protected INS in the NPs, thus reducing INS leakage.…”

Section: Discussionmentioning

confidence: 99%

pH-Responsive and Mucoadhesive Nanoparticles for Enhanced Oral Insulin Delivery: The Effect of Hyaluronic Acid with Different Molecular Weights

et al. 2023

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Drug degradation at low pH and rapid clearance from intestinal absorption sites are the main factors limiting the development of oral macromolecular delivery systems. Based on the pH responsiveness and mucosal adhesion of hyaluronic acid (HA) and poly[2-(dimethylamino)ethyl methacrylate] (PDM), we prepared three HA–PDM nano-delivery systems loaded with insulin (INS) using three different molecular weights (MW) of HA (L, M, H), respectively. The three types of nanoparticles (L/H/M-HA–PDM–INS) had uniform particle sizes and negatively charged surfaces. The optimal drug loadings of the L-HA–PDM–INS, M-HA–PDM–INS, H-HA–PDM–INS were 8.69 ± 0.94%, 9.11 ± 1.03%, and 10.61 ± 1.16% (w/w), respectively. The structural characteristics of HA–PDM–INS were determined using FT-IR, and the effect of the MW of HA on the properties of HA–PDM–INS was investigated. The release of INS from H-HA–PDM–INS was 22.01 ± 3.84% at pH 1.2 and 63.23 ± 4.10% at pH 7.4. The protective ability of HA–PDM–INS with different MW against INS was verified by circular dichroism spectroscopy and protease resistance experiments. H-HA–PDM–INS retained 45.67 ± 5.03% INS at pH 1.2 at 2 h. The biocompatibility of HA–PDM–INS, regardless of the MW of HA, was demonstrated using CCK-8 and live–dead cell staining. Compared with the INS solution, the transport efficiencies of L-HA–PDM–INS, M-HA–PDM–INS, and H-HA–PDM–INS increased 4.16, 3.81, and 3.10 times, respectively. In vivo pharmacodynamic and pharmacokinetic studies were performed in diabetic rats following oral administration. H-HA–PDM–INS exhibited an effective hypoglycemic effect over a long period, with relative bioavailability of 14.62%. In conclusion, these simple, environmentally friendly, pH-responsive, and mucoadhesive nanoparticles have the potential for industrial development. This study provides preliminary data support for oral INS delivery.

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Section: Discussionmentioning

confidence: 99%

pH-Responsive and Mucoadhesive Nanoparticles for Enhanced Oral Insulin Delivery: The Effect of Hyaluronic Acid with Different Molecular Weights

et al. 2023

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“…Lima et al [ 104 ] showed that surface chemistry and morphology are critical factors for the development of biomaterials. These materials can be applied to several cell adhesion applications, including rapid diagnostic, cell signaling, and biosensing mechanisms.…”

Section: Applicationsmentioning

confidence: 99%

Hyaluronic Acid. Extraction Methods, Sources and Applications

Graciela,

José Juan,

Gieraldin

et al. 2023

Polymers

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In this review, a compilation of articles in databases on the extraction methods and applications of hyaluronic acid (HA) was carried out. HA is a highly hydrated component of different tissues, including connective, epithelial, and neural. It is an anionic, linear glycosaminoglycan (GAG) primarily found in the native extracellular matrix (ECM) of soft connective tissues. Included in the review were studies on the extraction methods (chemical, enzymatical, combined) of HA, describing advantages and disadvantages as well as news methods of extraction. The applications of HA in food are addressed, including oral supplementation, biomaterials, medical research, and pharmaceutical and cosmetic industry applications. Subsequently, we included a section related to the structure and penetration routes of the skin, with emphasis on the benefits of systems for transdermal drug delivery nanocarriers as promoters of percutaneous absorption. Finally, the future trends on the applications of HA were included. This final section contains the effects before, during, and after the application of HA-based products.

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“…Layered spatially oriented chitosan-containing materials, which have been intensely studied recently, are promising for use in tissue engineering (bone tissue replacement), regenerative medicine (a temporary construct for restoring epithelial and connective tissue), and also for the development of controlled drug delivery systems [1][2][3][4][5]. Such materials are of particular importance to manufacture scaffolds [6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Structural and Morphological Features of Anisotropic Chitosan Hydrogels Obtained by Ion-Induced Neutralization in a Triethanolamine Medium

Shmakov,

Babicheva,

Kurochkina

et al. 2023

Gels

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For the first time, anisotropic hydrogel material with a highly oriented structure was obtained by the chemical reaction of polymer-analogous transformation of chitosan glycolate—chitosan base using triethanolamine (TEA) as a neutralizing reagent. Tangential bands or concentric rings, depending on the reaction conditions, represent the structural anisotropy of the hydrogel. The formation kinetics and the ratio of the positions of these periodic structures are described by the Liesegang regularities. Detailed information about the bands is given (formation time, coordinate, width, height, and formation rate). The supramolecular ordering anisotropy of the resulting material was evaluated both by the number of Liesegang bands (up to 16) and by the average values of the TEA diffusion coefficient ((15–153) × 10−10 and (4–33) × 10−10 m2/s), corresponding to the initial and final phase of the experiment, respectively. The minimum chitosan concentration required to form a spatial gel network and, accordingly, a layered anisotropic structure was estimated as 1.5 g/dL. Morphological features of the structural anisotropic ordering of chitosan Liesegang structures are visualized by scanning electron microscopy. The hemocompatibility of the material obtained was tested, and its high sorption–desorption properties were evaluated using the example of loading–release of cholecalciferol (loading degree ~35–45%, 100% desorption within 25–28 h), which was observed for a hydrophobic substance inside a chitosan-based material for the first time.

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Control of Surface Properties of Hyaluronan/Chitosan Multilayered Coatings for Tumor Cell Capture

Cited by 4 publications

References 71 publications

pH-Responsive and Mucoadhesive Nanoparticles for Enhanced Oral Insulin Delivery: The Effect of Hyaluronic Acid with Different Molecular Weights

pH-Responsive and Mucoadhesive Nanoparticles for Enhanced Oral Insulin Delivery: The Effect of Hyaluronic Acid with Different Molecular Weights

Hyaluronic Acid. Extraction Methods, Sources and Applications

Structural and Morphological Features of Anisotropic Chitosan Hydrogels Obtained by Ion-Induced Neutralization in a Triethanolamine Medium

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