Control of spindle polarity and orientation in Saccharomyces cerevisiae

Segal, Marisa; Bloom, Kerry

doi:10.1016/s0962-8924(01)01954-7

Cited by 102 publications

(86 citation statements)

References 46 publications

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“…Finally, we address the mechanism of rescue of cdc28-4 clb5⌬ lethality by the truncated SPC110. The mode of action of this dosage suppressor validates a model in which CDK may provide temporal control of astral MT organisation by the SPB, in coordination with spindle assembly, to promote correct spindle polarity (Segal and Bloom, 2001).…”

Section: ⌬13supporting

confidence: 55%

Phosphorylation of Spc110p by Cdc28p-Clb5p kinase contributes to correct spindle morphogenesis in S. cerevisiae

Huisman

Smeets

Segal

2007

Journal of Cell Science

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Spindle morphogenesis is regulated by cyclin-dependent kinases and monitored by checkpoint pathways to accurately coordinate chromosomal segregation with other events in the cell cycle. We have previously dissected the contribution of individual B-type cyclins to spindle morphogenesis in Saccharomyces cerevisiae. We showed that the S-phase cyclin Clb5p is required for coupling spindle assembly and orientation. Loss of Clb5p-dependent kinase abolishes intrinsic asymmetry between the spindle poles resulting in lethal translocation of the spindle into the bud with high penetrance in diploid cells. This phenotype was exploited in a screen for high dosage suppressors that yielded spc110Δ13, encoding a truncation of the spindle pole body component Spc110p (the intranuclear receptor for the γ-tubulin complex). We found that Clb5p-GFP was localised to the spindle poles and intranuclear microtubules and that Clb5p-dependent kinase promoted cell cycle dependent phosphorylation of Spc110p contributing to spindle integrity. Two cyclin-dependent kinase consensus sites were required for this phosphorylation and were critical for the activity of spc110Δ13 as a suppressor. Together, our results point to the function of cyclin-dependent kinase phosphorylation of Spc110p and provide, in addition, support to a model for Clb5p control of spindle polarity at the level of astral microtubule organisation.

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Section: ⌬13supporting

confidence: 55%

Phosphorylation of Spc110p by Cdc28p-Clb5p kinase contributes to correct spindle morphogenesis in S. cerevisiae

Huisman

Smeets

Segal

2007

Journal of Cell Science

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“…APC is distantly related to Kar9p, an S. cerevisiae protein that acts as a cue for orientation of the mitotic spindle to the bud, and associates with both the spindle pole body (the yeast "centrosome") and the actin cortex within the tip of a forming bud. 17,18 Excitingly, recent studies by Lechler and Fuchs have provided evidence for a similar process occurring during the stratification and differentiation steps of epidermal development in mammals. 19 In this case, both integrins and cadherins provided essential signals regulating the polarity complex (Par3 /Pins/aPKC), and additionally influence NuMA and dynactin activity, thus controlling the orientation of the centrosome and spindle.…”

Section: Roles Of the Centrosomementioning

confidence: 99%

HEF1-Aurora A Interactions: Points of Dialog between the Cell Cycle and Cell Attachment Signaling Networks

Pugacheva¹,

Golemis²

2006

Cell Cycle

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Regulated timing of cell division cycles, and geometrical precision in the planar orientation of cell division, are critical during organismal development and remain important for the maintenance of polarized structures in adults. Mounting evidence suggests that these processes are coordinated at the centrosome through the action of proteins that mediate both cell cycle and cell attachment. Our recent work identifying HEF1 as an activator of the Aurora A kinase suggests a novel hub for such integrated signaling. We suggest that defects in components of the machinery specifying the temporal and spatial integration of cell division may induce cancer and other diseases through pleiotropic effects on cell migration, proliferation, apoptosis, and genomic stability.

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“…Finally, a growing set of evidence shows that a complex pattern of cytoplasmic microtubule attachments with both the mother and the bud cortex as well as the bud neck is required for the orientation of the mitotic spindle along the mother-bud axis and ultimately for correct nuclear division (for a review, see Segal and Bloom, 2001). Cdc28 has been recently shown to interact with the plus end of cytoplasmic microtubules (Maekawa et al, 2003;Liakopoulos et al, 2003).…”

Section: Differential Localization Of Mitotic Cyclinsmentioning

confidence: 99%

Differential cellular localization among mitotic cyclins fromSaccharomyces cerevisiae: a new role for the axial budding protein Bud3 in targeting Clb2 to the mother-bud neck

Bailly

Cabantous

Sondaz

et al. 2003

Journal of Cell Science

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The mitotic cyclin Clb2 plays a major role in promoting M-phase in budding yeast, despite its functional redundancy with three closely related cyclins Clb1, Clb3 and Clb4. Here, we further investigate the mechanisms controlling the cellular distribution of Clb2 in living cells. In agreement with observations recently made by Hood et al. [Hood, J. K., Hwang, W. W. and Silver, P. A. (2001) J. Cell Sci. 114, 589-597], we find that GFP-tagged Clb2 expressed from its natural promoter localizes to various cellular compartments, including the nucleus, the mitotic spindle, the spindle pole bodies as well as the mother-bud neck. The neck localization is specific to Clb2 as Clb1, Clb3 and Clb4 are never observed there, even when over-expressed. Mutational analysis identifies a central region of Clb2, comprising residues 213-255 and a phylogenetically conserved hydrophobic patch, as an essential cis-acting determinant. Clb2 co-localizes with the bud site selection protein Bud3. Consistent with a role of Bud3 in targeting Clb2 to the bud neck, we report a two-hybrid interaction between these proteins. Furthermore, Clb2 is shown to be specifically delocalized in Δbud3 cells and in a bud3 mutant deleted for its C-terminal Clb2-interacting domain (bud3Δ1221), but not in a Δbud10 mutant. Correlating with this phenotype, bud3Δ1221 cells exhibit a pronounced (15-30 minutes) delay in cytokinesis and/or cell separation, suggesting an unanticipated function of Clb2 in these late mitotic events. Taken together, our data uncover a new role for Bud3 in cytokinesis that correlates with its capacity to target Clb2 at the neck, independently of its well established cell-type-specific function in bud site selection.

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Control of spindle polarity and orientation in Saccharomyces cerevisiae

Cited by 102 publications

References 46 publications

Phosphorylation of Spc110p by Cdc28p-Clb5p kinase contributes to correct spindle morphogenesis in S. cerevisiae

Phosphorylation of Spc110p by Cdc28p-Clb5p kinase contributes to correct spindle morphogenesis in S. cerevisiae

HEF1-Aurora A Interactions: Points of Dialog between the Cell Cycle and Cell Attachment Signaling Networks

Differential cellular localization among mitotic cyclins fromSaccharomyces cerevisiae: a new role for the axial budding protein Bud3 in targeting Clb2 to the mother-bud neck

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