Control of Somatostatin (SS) Secretion by CCK-1 and CCK-2 Receptors' Occupation in RIN-14B Cells, a Rat Pancreatic Islet Cell Line

El-Kouhen, Karim; Morisset, Jean

doi:10.1097/mpa.0b013e3181bea475

Cited by 7 publications

(1 citation statement)

References 39 publications

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“…Imunofluorescence microscopy with specific antibodies (22)(23)(24) showed that CCK-A receptors (top images), but not CCK-B receptors (bottom images), are more abundant in human acini within the pancreas slice ( Fig. 5A).…”

Section: Cck-a Receptors But Not Cck-b Receptors Are Present In Acimentioning

confidence: 99%

Ex vivo human pancreatic slice preparations offer a valuable model for studying pancreatic exocrine biology

Liang

Dolai

Xie

et al. 2017

Journal of Biological Chemistry

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A genuine understanding of human exocrine pancreas biology and pathobiology has been hampered by a lack of suitable preparations and reliance on rodent models employing dispersed acini preparations. We have developed an organotypic slice preparation of the normal portions of human pancreas obtained from cancer resections. The preparation was assessed for physiologic and pathologic responses to the cholinergic agonist carbachol (Cch) and cholecystokinin (CCK-8), including 1) amylase secretion, 2) exocytosis, 3) intracellular Ca responses, 4) cytoplasmic autophagic vacuole formation, and 5) protease activation. Cch and CCK-8 both dose-dependently stimulated secretory responses from human pancreas slices similar to those previously observed in dispersed rodent acini. Confocal microscopy imaging showed that these responses were accounted for by efficient apical exocytosis at physiologic doses of both agonists and by apical blockade and redirection of exocytosis to the basolateral plasma membrane at supramaximal doses. The secretory responses and exocytotic events evoked by CCK-8 were mediated by CCK-A and not CCK-B receptors. Physiologic agonist doses evoked oscillatory Ca increases across the acini. Supraphysiologic doses induced formation of cytoplasmic autophagic vacuoles and activation of proteases (trypsin, chymotrypsin). Maximal atropine pretreatment that completely blocked all the Cch-evoked responses did not affect any of the CCK-8-evoked responses, indicating that rather than acting on the nerves within the pancreas slice, CCK cellular actions directly affected human acinar cells. Human pancreas slices represent excellent preparations to examine pancreatic cell biology and pathobiology and could help screen for potential treatments for human pancreatitis.

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Section: Cck-a Receptors But Not Cck-b Receptors Are Present In Acimentioning

confidence: 99%