Control of silk microsphere formation using polyethylene glycol (PEG)

Wu, Jianbing; Zheng, Zhaozhu; Li, Gang; Kaplan, David L.; Wang, Xiaoqin

doi:10.1016/j.actbio.2016.05.019

Cited by 58 publications

(52 citation statements)

References 53 publications

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“…The loose porous structure facilitated water absorption and accelerated the dissolution of sponge. A large number of micro-and nano-sized particles were observed on the surface of SF-PEG gel sample (Figure 2(D)), likely due to PEG-induced SF nanoparticle and microparticle formation as reported in our previous study [25]. The presence of nano-and microparticles might have a beneficial effect on the mechanical properties of the SF-PEG gel, as these particles might help link the SF gel fibres due to large surface areas.…”

Section: Morphologies Of Sf and Sf-peg Spongessupporting

confidence: 72%

“…On the other hand, these particles might have a higher content of b-sheet structure compared to the SF gel fibres surrounding them, acting as mechanically and thermodynamically stable cores among the gel network. High content of b-sheet structure ($40%) in the PEG-induced SF nano-and microparticles have been demonstrated in our previous studies [25,26].…”

Section: Morphologies Of Sf and Sf-peg Spongessupporting

confidence: 53%

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Gellable silk fibroin-polyethylene sponge for hemostasis

Wei

Liu

Peng

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Traditional haemostatic materials generally have slow hemostasis rate and poor biocompatibility. This paper reports on the haemostatic properties and mechanism of silk fibroin (SF). SF-PEG sponge that could be solubilised and changed to gel form by blood was fabricated through mixing SF and polyethylene glycol (PEG, 1500 Da) followed by lyophilisation of the mixed solution. SF-PEG sponge, together with control samples of SF sponge (no PEG) and a commercially available haemostatic material, gelatine sponge, were subjected to the hemostasis tests using a liver trauma model of rabbit. The results showed that SF was superior to gelatine sponge in hemostasis time (136.17 ± 62.27 s and 249.83 ± 29.18 s) and blood loss (2.16 ± 1.27 g vs. 4.97 ± 1.44 g). Furthermore, in vitro experiments indicated SF-PEG sol-gel transition promoted platelet adhesion and aggregation, as well as platelet-fibrinogen interaction. Therefore, except for the physical blocking of bleeding port due to PEG-induced SF fast gelation, SF might also have an impact on blood coagulation process, a phenomenon that has not been reported before. In conclusion, SF is a new type of haemostatic material that might be able to meet the requirements of speed, efficiency and biosafety in a variety of clinical applications.

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Section: Morphologies Of Sf and Sf-peg Spongessupporting

confidence: 72%

Section: Morphologies Of Sf and Sf-peg Spongessupporting

confidence: 53%

Gellable silk fibroin-polyethylene sponge for hemostasis

Wei

Liu

Peng

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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“…The FTIR spectra of ethanol‐treated membranes are presented in Figure B. The results indicated that the SF in the membranes had a predominant β‐sheet structure, as bands of SF had an shift from 1646 to 1627 cm −1 and from 1535 to 1519 cm −1 after ethanol treatment, consistent with the literature . In curves c and d, characteristic peaks at 1100 cm −1 (C–O stretching vibrations) were observed in the samples, which can be associated with the carboxylate groups.…”

Section: Resultssupporting

confidence: 84%

Silk Fibroin‐Based Fibrous Anal Fistula Plug with Drug Delivery Function

Xie

Liu

Zheng

et al. 2018

Macromolecular Bioscience

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The aim of this work is to develop a drug-loaded silk fibroin fibrous membrane (DSFM) that can be attached to the surface of an anal fistula plug to improve the treatment of Crohn's disease (CD). Curcumin (CUR) and 5-aminosalicylic acid (5-ASA)-loaded silk fibroin (SF) membranes are coaxially electrospun onto the surface of a braided silk filament plug. The membranes show a predominant structure of random coil and silk I conformation. The concentration of CUR/5-ASA (weight ratio of 1/1) in the SF solution is optimized to 0.4, 0.9, and 1.9 wt%. The morphologies, secondary structures, and in vitro drug release properties of the membranes are examined. Sectional images of fibers in the membranes show core-shell structures. The coaxial electrospinning process does not alter the chemical characteristics of the drugs. The dual-drugs encapsulated in the membranes are released in a steady and sustainable manner, and the cumulative release rate is improved by the increased drug loading. The membranes exhibit no cytotoxicity, thereafter increase the viability of human fibroblasts on the DSFMs. These SF membranes with core-shell structure and functional encapsulation of CUR and 5-ASA should be useful for further studies toward the treatment of CD.

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“…The unreleased heparin might have been entrapped in the SF coating due to steric hindrance or binding to the SF matrix and is expected to be released by enzymatic degradation of SF during in vivo applications. This result demonstrates the vital role of β-sheet structure and crystallinity of SF for controllable drug release and is consistent with the results from previous studies [44]. decreased.…”

Section: Heparin Release and Hvsm Cells Viability Of Modified Bsgssupporting

confidence: 93%

A heparin-functionalized woven stent graft for endovascular exclusion

Zheng

Chen

et al. 2019

Colloids and Surfaces B: Biointerfaces

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Highlights  A novel seamless bifurcate stent graft (BSG) was developed to avoid the leakage of traditional stent graft.  The BSG with excellent physical properties, which can meet the requirements of transplantation in endovascular graft exclusion.  A very easy drug modification approach by coating mixtures of heparin and silk fibroin solution onto BSG surface was studies.  The cell viability in heparin release of modified BSGs was studied and analyzed. 3 Abstract：With the increase of vascular diseases in recent years, it is of importance to develop an anti-occlusion stent graft, which can meet the requirements of transplants for a long term. In this paper, we describe a silk fibroin (SF)/heparin-functionalized bifurcated stent graft (BSG) using textile forming technology. The BSGs were prototyped based on seamless weaving technology, and the surface was modified with SF-loaded heparin under steam/air treatment to improve their patency. The physical properties such as thickness, water permeability, contact angle, mechanical properties, and in vitro drug release and coagulation time of the BSGs were examined. The results showed that heparin modification can improve its coagulation time, and the water permeability resistance of the BSGs reached 1.154 ± 0.854 mL/(cm 2 ×min), while their thicknesses were just 0.085 ± 0.004 mm. The heparin release of the BSGs showed that the release time was prolonged upon steam treatment by means of the increase in the β-sheet structure and crystallinity of SF. The viability and attachment of human vascular smooth muscle (HVSM) cells cultured in the release of modified BSGs demonstrated that the modified BSGs could significantly inhibit the proliferation of HVSM cells. The heparin-functionalized BSG with satisfactory thickness, water permeability resistance and anti-occlusion function, which has potential applications in the treatment of vascular diseases.

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Control of silk microsphere formation using polyethylene glycol (PEG)

Cited by 58 publications

References 53 publications

Gellable silk fibroin-polyethylene sponge for hemostasis

Gellable silk fibroin-polyethylene sponge for hemostasis

Silk Fibroin‐Based Fibrous Anal Fistula Plug with Drug Delivery Function

A heparin-functionalized woven stent graft for endovascular exclusion

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