Control of senescence by polyamines and guanidines in young and mature barley leaves

Peroxidase of Kiwifruit

“…Peroxidase activity (POD) was determined spectrophotometrically at 460 nm using odianisidine as chromogenic indicator (Srivastava et al, 1983). The total reaction volume was 3 mL containing: 2.7 mL 50 mM sodium acetate, pH 6 buffer; 0.1 mL 0.5% hydrogen peroxide; 0.1 mL enzyme extract and the addition of 0.1 mL 0.25% 0-dianisidine (w/v).…”

High-oxygen and high-carbon dioxide containing atmospheres inhibit growth of food associated moulds

“…Peroxidase activity was measured according to the (modified) method used by Srivastava et al (1983), adding 0AE03 ml 1% H 2 O 2 , 0AE03 ml 0AE25% o-dianisidine (Sigma-Aldrich) and 0AE03 ml cell-free extract to 0AE9 ml NaOAc buffer (50 mmol l )1 , pH 6). After 1 min at 20°C, 0AE1 ml H 2 SO 4 (5 mol l )1 ) was added to terminate the reaction.…”

Polyamines and carnation flower senescence: Endogenous levels and the effect of applied polyamines on senescence

“…Researchers have shown that STS, some cytokinins, ethanol as well as the ethylene inhibitors AOA and AVG can delay senescence of cut carnations [4,5,7,10,14,15,21,22,23,25] . Polyamines, ubiquitous in plants and known to be associated with cell division and growth, have been shown to act as anti-senescence agents in a number of tissues [2,9,11,12,13,16,17,18,19,20]. The most commonly known of these polycationic, bioregulatory compounds are putrescine, spermidine and spermine .…”