Control of root architecture and nodulation by the<i>LATD/NIP</i>transporter

Harris, Jeanne M.; Dickstein, Rebecca

doi:10.4161/psb.5.11.13165

Cited by 32 publications

(28 citation statements)

References 39 publications

(68 reference statements)

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“…In M. truncatula, it has been shown that MtNPF1.7 is essential in the formation and maintenance of nodule meristems and in rhizobial invasion during nodulation (Veereshlingam et al 2004;Harris and Dickstein 2010). Using different mutants of MtNPF1.7, Bagchi et al (2012) proposed that the ability of MtNPF1.7 to transport nitrate is correlated with the abilities of mutants to form and maintain nodules.…”

Section: Nitrate Transporters and Their Possible Roles In Rhizobial Smentioning

confidence: 99%

Nitrate transporters: an overview in legumes

Pellizzaro

Alibert

Planchet

et al. 2017

Planta

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Main conclusion The nitrate transporters, belonging to NPF and NRT2 families, play critical roles in nitrate signaling, root growth and nodule development in legumes.Nitrate plays an essential role during plant development as nutrient and also as signal molecule, in both cases working via the activity of nitrate transporters. To date, few studies on NRT2 or NPF nitrate transporters in legumes have been reported, and most of those concern Lotus japonicus and Medicago truncatula. A molecular characterization led to the identification of 4 putative LjNRT2 and 37 putative LjNPF gene sequences in L. japonicus. In M. truncatula, the NRT2 family is composed of 3 putative members. Using the new genome annotation of M. truncatula (Mt4.0), we identified, for this review, 97 putative MtNPF sequences, including 32 new sequences relative to previous studies. Functional characterization has been published for only two MtNPF genes, encoding nitrate transporters of M. truncatula. Both transporters have a role in root system development via abscisic acid signaling: MtNPF6.8 acts as a nitrate sensor during the cell elongation of the primary root, while MtNPF1.7 contributes to the cellular organization of the root tip and nodule formation. An in silico expression study of MtNPF genes confirmed that NPF genes are expressed in nodules, as previously shown for L. japonicus, suggesting a role for the corresponding proteins in nitrate transport, or signal perception in nodules. This review summarizes our knowledge of legume nitrate transporters and discusses new roles for these proteins based on recent discoveries.

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Section: Nitrate Transporters and Their Possible Roles In Rhizobial Smentioning

confidence: 99%

Nitrate transporters: an overview in legumes

Pellizzaro

Alibert

Planchet

et al. 2017

Planta

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“…Nitrate treatment induced the accumulation of the small RNA miR393, which was shown to specifically direct cleavage of AFB3 transcripts (Vidal et al, 2010a). In M. truncatula, nitrate-induced inhibition of primary root growth was altered in a mutant affected in the high-affinity nitrate transporter MtNPF1.7, also known as Lateral Root Organ Defective (LATD)/Numerous Infections and Polyphenolics (Harris and Dickstein, 2010;Yendrek et al, 2010;Bagchi et al, 2012). Because the root architecture phenotype of npf1.7 mutants was rescued by the application of exogenous abscisic acid (ABA; Liang et al, 2007), it is possible that the control of root architecture by nitrate in M. truncatula might involve an interaction with an ABA signaling pathway.…”

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The Nitrate Transporter MtNPF6.8 (MtNRT1.3) Transports Abscisic Acid and Mediates Nitrate Regulation of Primary Root Growth in Medicago truncatula

et al. 2014

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Elongation of the primary root during postgermination of Medicago truncatula seedlings is a multigenic trait that is responsive to exogenous nitrate. A quantitative genetic approach suggested the involvement of the nitrate transporter MtNPF6.8 (for Medicago truncatula NITRATE TRANSPORTER1/PEPTIDE TRANSPORTER Family6.8) in the inhibition of primary root elongation by high exogenous nitrate. In this study, the inhibitory effect of nitrate on primary root elongation, via inhibition of elongation of root cortical cells, was abolished in npf6.8 knockdown lines. Accordingly, we propose that MtNPF6.8 mediates nitrate inhibitory effects on primary root growth in M. truncatula. pMtNPF6.8:GUS promoter-reporter gene fusion in Agrobacterium rhizogenes-generated transgenic roots showed the expression of MtNPF6.8 in the pericycle region of primary roots and lateral roots, and in lateral root primordia and tips. MtNPF6.8 expression was insensitive to auxin and was stimulated by abscisic acid (ABA), which restored the inhibitory effect of nitrate in npf6.8 knockdown lines. It is then proposed that ABA acts downstream of MtNPF6.8 in this nitrate signaling pathway. Furthermore, MtNPF6.8 was shown to transport ABA in Xenopus spp. oocytes, suggesting an additional role of MtNPF6.8 in ABA root-to-shoot translocation. NO 3 2-influx experiments showed that only the inducible component of the low-affinity transport system was affected in npf6.8 knockdown lines. This indicates that MtNPF6.8 is a major contributor to the inducible component of the low-affinity transport system. The short-term induction by nitrate of the expression of Nitrate Reductase1 (NR1) and NR2 (genes that encode two nitrate reductase isoforms) was greatly reduced in the npf6.8 knockdown lines, supporting a role of MtNPF6.8 in the primary nitrate response in M. truncatula.

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“…S3). We and others have speculated that MtNIP/LATD may be a NO 3 2 transceptor or sensor (Harris and Dickstein, 2010;Yendrek et al, 2010;Gojon et al, 2011). If it is a NO 3 2 transceptor or sensor, we predict that it may be responsible for highaffinity NO 3 2 sensing.…”

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confidence: 99%

“…The M. truncatula MtNRT1.3 transporter was shown to be a dualaffinity NO 3 2 transporter; MtNRT1.3 is up-regulated by the absence of NO 3 2 (Morère-Le Paven et al, 2011). The M. truncatula NIP/LATD (for Numerous Infections and Polyphenolics/Lateral root-organ Defective) gene encodes a predicted NRT1(PTR) transporter (Harris and Dickstein, 2010;Yendrek et al, 2010), and the three known nip and latd mutants have pleiotropic defects in nodulation and root architecture. Mtnip-1, containing a missense (A497V) mutation in one of the NIP/LATD protein's transmembrane domains, is well characterized with respect to nodulation phenotypes (Veereshlingam et al, 2004).…”

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Functional Assessment of the Medicago truncatula NIP/LATD Protein Demonstrates That It Is a High-Affinity Nitrate Transporter

et al. 2012

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The Medicago truncatula NIP/LATD (for Numerous Infections and Polyphenolics/Lateral root-organ Defective) gene encodes a protein found in a clade of nitrate transporters within the large NRT1(PTR) family that also encodes transporters of dipeptides and tripeptides, dicarboxylates, auxin, and abscisic acid. Of the NRT1(PTR) members known to transport nitrate, most are lowaffinity transporters. Here, we show that M. truncatula nip/latd mutants are more defective in their lateral root responses to nitrate provided at low (250 mM) concentrations than at higher (5 mM) concentrations; however, nitrate uptake experiments showed no discernible differences in uptake in the mutants. Heterologous expression experiments showed that MtNIP/LATD encodes a nitrate transporter: expression in Xenopus laevis oocytes conferred upon the oocytes the ability to take up nitrate from the medium with high affinity, and expression of MtNIP/LATD in an Arabidopsis chl1(nrt1.1) mutant rescued the chlorate susceptibility phenotype. X. laevis oocytes expressing mutant Mtnip-1 and Mtlatd were unable to take up nitrate from the medium, but oocytes expressing the less severe Mtnip-3 allele were proficient in nitrate transport. M. truncatula nip/latd mutants have pleiotropic defects in nodulation and root architecture. Expression of the Arabidopsis NRT1

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Control of root architecture and nodulation by theLATD/NIPtransporter

Cited by 32 publications

References 39 publications

Nitrate transporters: an overview in legumes

Nitrate transporters: an overview in legumes

The Nitrate Transporter MtNPF6.8 (MtNRT1.3) Transports Abscisic Acid and Mediates Nitrate Regulation of Primary Root Growth in Medicago truncatula

Functional Assessment of the Medicago truncatula NIP/LATD Protein Demonstrates That It Is a High-Affinity Nitrate Transporter

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