Control of Relative Air Humidity as a Potential Means to Improve Hygiene on Surfaces: A Preliminary Approach with Listeria monocytogenes

Zoz, Fiona; Iaconelli, Cyril; Läng, Émilie; Iddir, Hayet; Guyot, Stéphane; Grandvalet, Cosette; Gervais, Patrick; Beney, Laurent

doi:10.1371/journal.pone.0148418

Cited by 43 publications

(28 citation statements)

References 48 publications

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“…), while a decrease in RH has demonstrated a decreased survival of L. monocytogenes (Zoz et al . ). Conversely, reduction in RH has been shown to enhance transfer of L. monocytogenes from biofilm to meat products potentially due to increased capillary action within the food (Rodríguez et al .…”

Section: Introductionmentioning

confidence: 97%

“…Although surface characteristics such as roughness and wettability are important variables when considering survival of micro-organisms on steel, other environmental conditions are likely to play a key role. An increase in relative humidity (RH), a measure relating to amount of water vapour in the atmosphere, has been shown to prolong survival of L. monocytogenes, as well as encourage growth when inoculated on fresh produce (Likotrafiti et al 2013), while a decrease in RH has demonstrated a decreased survival of L. monocytogenes (Zoz et al 2016). Conversely, reduction in RH has been shown to enhance transfer of L. monocytogenes from biofilm to meat products potentially due to increased capillary action within the food (Rodr ıguez et al 2007).…”

Section: Introductionmentioning

confidence: 99%

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Effect of humidity and temperature on the survival ofListeria monocytogeneson surfaces

Redfern

2017

Lett Appl Microbiol

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Understanding survival of potential food-borne pathogens is essential for the safe production and preparation of food. While it has long been 'common knowledge' that relative humidity can affect the growth and survival of micro-organisms, this study systematically describes the survival of L. monocytogenes on stainless steel under varying humidity and temperatures for the first time. The outcomes from this paper will allow those involved with food manufacture and preparation to make informed judgement on environmental conditions relating to humidity control, which is lacking in the food standards guidelines.

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Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Effect of humidity and temperature on the survival ofListeria monocytogeneson surfaces

Redfern

2017

Lett Appl Microbiol

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“…The results indicate that rapid rehydration of WT strain cells was more detrimental than progressive rehydration. It has been reported that progressive rehydration could reduce the stress applied to bacteria as it promotes membrane integrity recovery (58, 59). We found only seven sensitive mutants whatever their rehydration kinetics (Table 4).…”

Section: Discussionmentioning

confidence: 99%

Identification and expression ofLactobacillus paracaseigenes for adaptation to desiccation and rehydration

Palud

Salem

Cavin

et al. 2018

Preprint

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12Lactobacillus paracasei is able to persist in a variety of natural and technological 13 environments despite physico-chemical perturbations, in particular alternations between 14 desiccation and rehydration. However, the way in which it adapts to hydric fluctuations and 15 in particular the genetic determinants involved are not clearly understood. To identify the 16 genes involved in adaptation to desiccation, an annotated library of L. paracasei random 17 transposon mutants was screened for viability after desiccation (25% relative humidity, 18 25°C). Subsequently, the expression of the identified genes was measured at five stages of the 19 dehydration-rehydration process to formulate the chronology of gene expression. The 24 20 identified genes were related to metabolism and transport, membrane function and structure, 21 regulation of stress response, DNA related enzymes and environmental sensing. They were classified into four different transcriptomic profiles, in particular genes upregulated during 23 both desiccation and rehydration phases and genes upregulated during the desiccation phase 24 only. Thus, genetic response to hydric fluctuations seems to occur during desiccation and can 25 continue or not during rehydration. The genes identified should contribute to improving the 26 stabilization of lactobacillus starters in dry state. 27 28 Importance 29Since water is the fundamental component of all living organisms, desiccation and 30 rehydration alternation is one of the most prevalent and severe stresses for most 31 microorganisms. Adaptation to this stress occurs via a combination of mechanisms which 32 depend on the genetic background of the microorganism. In L. paracasei, we developed a 33 strategy to identify genes involved in the adaptation to hydric fluctuations using random 34 transposon mutagenesis and targeted transcriptomics. Both dehydration and rehydration were 35 studied to decipher the chronology of genetic mechanisms. We found 24 as yet unidentified 36 genes involved in this response. Most of them are linked to either the transport of molecules 37 or to cell wall structure and function. Our screening also identified genes for environment 38 sensing and two alarmones necessary for L. paracasei survival. Furthermore, our results show 39 that desiccation is a critical phase for inducing stress response in L. paracasei. 40 41 expression 43 44 45 46 47Water is essential for all living organisms as it contributes to the structure of cells, stabilizes 48 proteins, lipids and nucleic acids, and maintains vital metabolic systems and chemical 49 reactions (1). Desiccation leads to water exit from cells, induces structural modifications, and 50 causes osmotic and oxidative stresses (1-3). In addition, the rehydration phase could lead to 51 membrane alterations (4). The capacity to return to life after hydric fluctuations is not only 52 crucial for bacteria as a function of diurnal and seasonal cycles in natural environments but 53 also during food industry processes such as conventional drying, f...

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“…Analysis of Variance (ANOVA) was performed to assess the statistical significance between input and output variables and estimate the regression coefficients of prediction models. Means separation for the data was made at p=0.05 level and adjusted by Turkey-Kramer's Honestly Significant Difference (HSD) [27]. The p-value was used to assess the significant level of prediction models and the validity of the regression coefficients.…”

Section: Statistical Analysis and Modelingmentioning

confidence: 99%

Development of droplet characteristics prediction models for air induction nozzles based on wind tunnel tests

Liao

Hewitt

Wang

et al. 2019

International Journal of Agricultural and Biological Engineering

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Nozzle flowrate and spray pressure are two of the most important factors influencing on droplet characteristics. With the aim to develop prediction models for air-induction nozzles (AINs), a series of Billericay Farm Services (BFS) AINs with different orifice diameters in combination with tap water were tested. 0.2 MPa, 0.3 MPa, 0.4 MPa, 0.5 MPa, 0.6 MPa and 0.7 MPa of spray pressures and 2 m/s, 3 m/s, 4 m/s and 5 m/s of air speeds were setup. Based on the wind tunnel tests data, prediction models with input variables of nozzle flowrate and spray pressure and output variables of D v0.1 , D v0.5 , D v0.9 , %<150 µm (proportion of spray volume contained in droplets with diameter below 150 µm), relative span (RS) and coefficient of variation (CV) of D v0.5 were developed. The developed models were validated based on wind tunnel experimental data. Results showed that: for D v0.1 , D v0.5 , D v0.9 and %<150 µm, R 2 were equal to 0.768, 0.823, 0.868 and 0.811, indicating that the predictive ability for these four parameters is strong. For RS and CV, R 2 were equal to 0.100 and 0.113, respectively, indicating that the predictive ability for these two parameters is poor. The models developed in the present study are helpful for facilitating the use of AIN in agricultural spray application. et al. Development of droplet characteristics prediction models for air induction nozzles based on wind tunnel tests. Int J Agric & Biol Eng, 2019; 12(6): 1-6.

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Control of Relative Air Humidity as a Potential Means to Improve Hygiene on Surfaces: A Preliminary Approach with Listeria monocytogenes

Cited by 43 publications

References 48 publications

Effect of humidity and temperature on the survival ofListeria monocytogeneson surfaces

Effect of humidity and temperature on the survival ofListeria monocytogeneson surfaces

Identification and expression ofLactobacillus paracaseigenes for adaptation to desiccation and rehydration

Development of droplet characteristics prediction models for air induction nozzles based on wind tunnel tests

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