Control of powdery mildew with saponins in extracts of quillay (Quillaja saponaria).

Apablaza, G.; Díaz, Martina; Martín, Ricardo San; Moya, Esteban A.

doi:10.7764/rcia.v29i2.417

Cited by 8 publications

(9 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Antifungal effects of commercial Q. saponaria extracts against G. graminis var. tritici have an in vitro effect at concentrations of 50 to 500 mg L -1 of saponins (Apablaza et al, 2002;. This concentration range is associated with hyphae deformation, mycelium thinning, and breakage of mycelia strands between fungus hyphae, as reported by Apablaza et al (2008).…”

Section: Discussionmentioning

confidence: 53%

“…This concentration range is associated with hyphae deformation, mycelium thinning, and breakage of mycelia strands between fungus hyphae, as reported by Apablaza et al (2008). This effect is attributed to the presence of triterpenic saponins, which show fungicidal activity associated with the membranolytic actions on the sterols present in the fungal cell membranes (Apablaza et al, 2002;Ribera et al, 2008;Augustin et al, 2011). Antifungal properties of saponin are attributed to aglycones present in these molecules and their ability to form a complex with sterols of the cell membranes of fungi, causing pore formation and loss of membrane integrity (Osbourn, 2003;Augustin et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…Saponins are secondary triterpenoid metabolites produced in many plant species, some with antifungal activity (Augustin et al, 2011;Ahmed et al, 2012). Various studies have shown a control effect by Q. saponaria on different species of Oidium (Apablaza et al, 2002;Moya et al, 2010), as well as on other phytopathogenic fungi such as Botrytis cinerea Pers.:Fr. (Ribera et al, 2008) and Gaeumannomyces graminis (Sacc.)…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of saponins of Quillaja saponaria extracts in combination with Pseudomonas protegens to control Gaeumannomyces graminis var. tritici in wheat

González-Castillo¹,

Quezada-D'Angelo²,

Silva-Aguayo³

et al. 2018

Chil. j. agric. res.

View full text Add to dashboard Cite

Extracts rich in saponins from Quillaja saponaria Mol. and populations of rhizobacteria from the genus Pseudomonas, which produce antimicrobial compounds, have been associated with reduction of the fungus Gaeumannomyces graminis var. tritici, the causal agent of take-all disease, which is responsible for severe loss of wheat (Triticum aestivum L.) crops worldwide. However, there is a limited background on the interaction between these bacteria and natural triterpenoids. The aim of this research was to assess the effect of saponin rich extract on Pseudomonas protegens strains 2,4-diacetylphloroglucinol-producers under in vitro and in plant conditions and determining the synergistic effect to be used together to control G. graminis var. tritici in wheat plants. We determined that 8% and 90% of saponins rich Q. saponaria extracts have a differential effect on P. protegens according to their purity (P ≤ 0.05). On wheat seedlings, quillaja extract with 90% of saponins did not affect the three antagonistic bacterial strain populations assessed, but affected biofilm formation at saponins concentration of 7360 mg L-1. Pseudomonas protegens strains had a variable antagonist activity in wheat plants, and controlled the fungus when were combined with different concentrations of pure Q. saponaria extract, with the concentration of 1840 ppm reducing the take-all disease in 32.5% with respect to the control inoculated with G. graminis var. tritici (P ≤ 0.05). However, no synergistic effects when the plant extract was combined with the bacterial strains were observed. These results showed the promising and complexity of combining bacterial and plant extract to develop a biopesticide, which could control this fungal disease.

show abstract

Section: Discussionmentioning

confidence: 53%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of saponins of Quillaja saponaria extracts in combination with Pseudomonas protegens to control Gaeumannomyces graminis var. tritici in wheat

González-Castillo¹,

Quezada-D'Angelo²,

Silva-Aguayo³

et al. 2018

Chil. j. agric. res.

View full text Add to dashboard Cite

show abstract

“…(Gandhi and Maheshwari, 2000;Ismail, et al, 2003 andThind, et al, 2004). Furthermore, Bacillus subtilis, Pseudomonas fluorescens, Trichoderma harzianum, is an efficient bio-agent that protects cucurbits plants against powdery mildew disease (Bettiol, et al, 19972;Urquhart et al, 1994;Koumaki, et al, 2001;Apablaza, et al, 2002;Levy, et al, 2004;Schmitt, 2006 andZhang, et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Management of Powdery Mildew Disease on Squash by Using Biological Agents and Some Chemical Inducers

Abd-El-Sayed

Eisa

2009

Journal of Plant Protection and Pathology

View full text Add to dashboard Cite

Three biocontrol agents i.e., Bacillus subtilis, Pseudomonas fluorescens, Trichoderma harzianum, were evaluated for their efficacy in controlling powdery mildew on squash plants. In greenhouse experiment, the highest inhibition of disease severity percentage was achieved by P. fluorescens, B. subtilis and T. harzianum, and application of Amistar 250 SC on squash plants resulted significant reduction of disease severity. The bacterial bioagents, i.e. P. fluorescens, and B. subtilis exhibited the highest reduction in disease severity. On the other hand, T. harzianum gave the lowest reduction in the disease severity. All resistance inducers (i.e., potassium nitrate (KNO3), potassium phosphate monobasic (KH2PO4), potassium phosphate dibasic (K2HPO4 and salicylic acid) treatments resulted in significant reduction in disease severity under greenhouse conditions. Under field conditions, similar reduction in disease severity of powdery mildew was observed in treated plants. All treatments significantly increased squash yield per plant. More than 75% increase in yield was observed in treated plants with (KNO3 at 100 mM).

show abstract

“…Plants possess extensive qualitative and quantitative bioactive molecule variations that are potentially therapeutic (Oksman-Caldentey and Inzé, 2004;Gershenzon and Dudareva, 2007). Research into the biological activity of crude extracts, essential oils, and metabolites obtained from plants has demonstrated a capacity to control various fungal phytopathogens (Isman, 2000;Lazzeri and Manici, 2000;Wedge et al, 2000;Apablaza et al, 2002;Park et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Crude extracts of Drimys winteri bark to inhibit growth of Gaeumannomyces graminis var. tritici

Zapata¹,

Vargas²,

Monsálvez³

et al. 2011

Chilean J. Agric. Res.

View full text Add to dashboard Cite

The aim of this study was to assess the effect of Drimys winteri J.R. Forst. & G. Forst. bark and its extracts, obtained sequentially with n-hexane, acetone, and methanol, against Gaeumannomyces graminis var. tritici (Ggt). Ground bark of D. winteri was mixed with potato dextrose agar growth media at concentrations of 250, 500, 1000, 2000, and 4000 mg L -1 in Petri plates. Each extract was mixed at concentrations of 100, 200, 400, and 800 mg L -1 . Petri plates were inoculated in the center with a 5-mm mycelium disk and were incubated at 24 ± 1 ºC. Daily measurements of mycelium radial growth were taken to determine growth rate and growth inhibition (%). A ground bark concentration of 978 mg L -1 was needed to inhibit Ggt growth by 50%, while extracts obtained with n-hexane and acetone only required 198 and 234 mg L -1 , respectively. The methanol extract only inhibited Ggt growth by 33% when tested at the highest concentration. In conclusion, ground bark and crude extracts, obtained sequentially with n-hexane, acetone, and methanol, from D. winteri bark inhibit Ggt growth when applied in vitro. The n-hexane extract showed the highest inhibitory growth activity.

show abstract

Control of powdery mildew with saponins in extracts of quillay (Quillaja saponaria).

Cited by 8 publications

References 3 publications

Effect of saponins of Quillaja saponaria extracts in combination with Pseudomonas protegens to control Gaeumannomyces graminis var. tritici in wheat

Effect of saponins of Quillaja saponaria extracts in combination with Pseudomonas protegens to control Gaeumannomyces graminis var. tritici in wheat

Management of Powdery Mildew Disease on Squash by Using Biological Agents and Some Chemical Inducers

Crude extracts of Drimys winteri bark to inhibit growth of Gaeumannomyces graminis var. tritici

Contact Info

Product

Resources

About